CAMK2B (NM_001220) Human 3' UTR Clone

CAT#: SC218593

3' UTR clone of calcium/calmodulin-dependent protein kinase II beta (CAMK2B) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CAMK2B
• Synonyms: CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54
• ACCN: NM_001220
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC218593 3’UTR clone of NM_001220
  The sequence shown below is from the reference sequence of NM_001220. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCGGGCGCGCCTGTGGCCCCGCTGCAGTGAAGAGCTGCGCCCTGGTTTCGCCGGACAGAGTTGGTGTTT
  GGAGCCCGACTGCCCTCGGGCACACGGCCTGCCTGTCGCATGTTTGTGTCTGCCTCGTTCCCTCCCCTG
  GTGCCTGTGTCTGCAGAAAAACAAGACCAGATGTGATTTGTTAAAAAAAAACAAAAAAAAAAAAAAAAA
  AAACAAGATGACGACGACAACCACAAAAAAAATTGACATCAGATGAAATGAAAAAAAAAAAAAACAAAA
  AAAACTAAAGGAAGGAAAAAGCTGTAAAAATCACTGGCATTCGTGGGGCCACTCCCCACCCAAGCTCCA
  CGTGTGTCCGTCTGTGCTCCTGGCCTCTGGGGGACCAGCTGGGACATGAACTTGTCTGCCAGGCCCCCG
  TCGCGTGCTGAACGGTGTTAGTTTGTAGGTAACGCACACACCCCACACCTAAGGTGTCTGCATCCTCCT
  GCCAACGCATGGGCTCCACGTGGTGTGCTCGCTGGCTGTCGTGACTGTCAGCTGTCTCTTGGGAGGGGC
  TGTGGGGGCCCGCTGGGCTGCCTCCTTTCCCGCTAGTTGTGCCTGAGAGTTGCTGTTGTTCCTGCTTTC
  CCTTCCCTTCCTTTCATCCCCTGAAGGGCTAGGTGTGGGTTTTCCGTGCCCGGTATCCCCACACACCCA
  GCACGGACAACCCTTCGGCAGAGCCCAGGCCGGCCCCTCACCCCCTGGAGTATTGAAACTGGAGTCCCG
  TCCCCAAGGCCTTCAGAGATGCCCCTACACACCCAGGGCTCCAGCTCTGGTCCTTCTGGGGGAGTAAAG
  TGCAAAGAGGGGCACAGCTTAGTTTTGGGCCTCTCGCCGAGCAAGAGACAGCACTGCTGGCTACAGCTC
  CAACACAGCCAGCTGTGGCAAGAGGACTCTGCCTGGGCTGGCCCCCCTCCTGTGTGAGGTGTCTGTCCC
  TTCTCTGCTGGCCAGCAGCAGATGCACTGGCAGCTCCCAACCCTGTTTCCGCCCCTCGGCCCTCCCCCA
  GCCTGTTCGGCTTCTCTGCAGCCCGCAAGGGGGAGCAGACTTTTGACAAAGGACTGCGGGCCTCGCTCA
  AGTCCCTGAGCCCCCAGCTGAAGCTGGGAGGGGAGGCCAGGCTTTGTGTCTGGGCATATTCGTCTGCTG
  ATGGGGTTTGGGGAAGCCTGGGGCTTGGGGTTTGGTCGGGTGGTGCAGCTAGTGGCAGAGCGGGATCAG
  AGGTGGTGGCTGCCCAGCTTCTGGGCTGAGACAAGGGTCTGTGCAGGGGTTTACTGAAGTGGGAGTGCC
  TTTGGAATCTGGGCCGGGAGCAGAAGGGAGCAAAAGCTACAGTGGGAGCCAGCCTAGGGCACATGGGAG
  GCGTGAGGGCAGTGCTGCCCGTGCAGTGTCAGGTGTGCCAGTGCCTTGGCGGGCTGCAGTGCGTGTGAG
  GGCACCTTCTAGGTGGGCCAGGGATGCAGCTATGGAGATAAGGCGGGCTGGGGACAGAAACAGGTGGGC
  ACAGGGCCCAGGACACCAGCGGATGGAGGGCAGGGTCTAGCCCTGTGCTCCTGAGCGTCGGCTGCCTGG
  GTTCGAGGCGGTGGGTCCCCGGCCCCTTGTGATGGTGTGTACCATGGGGGAGCTCGGGGACAGGGCAAG
  CCCGAGCATGGTGGGGCTGCAGGGTGGGTCTGAAGCCAGGTTGGGTGGGGGTGGTCACAAGCCCTGACT
  GCAGAGGGTCAGGGGCTCCTGCCCCAGTGCCTGCCCACTTTCAATTCACATTGTTTTCAACAAGGATTT
  TCTTTATCTTCCCCTACAAATCAAGCCAAGGGAGGGGCACAGAATGGGGAACAGGACACAGGATCCTAA
  ACTCCAAGGGGACTGTCCACCGATGAACACTCAGAGTGGACACCATCTTCCGTCCACGCTGTGCCCAGG
  ACAGCTGTCCCCATCCATGAACACAGGGTAAACATCTGCCGGGCTCCGCACCAGTGGCTCCCTGGGCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001220.5
• Summary: The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
• Locus ID: 816
• MW: 71.4