ROCK2 (NM_004850) Human 3' UTR Clone

CAT#: SC216436

3' UTR clone of Rho-associated coiled-coil containing protein kinase 2 (ROCK2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ROCK2
• Synonyms: ROCK-II
• ACCN: NM_004850
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC216436 3’UTR clone of NM_004850
  The sequence shown below is from the reference sequence of NM_004850. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGACAGCTTGCCCCAAACAAACCTAGCTAACTGCCTTCTATGAAAGCAGTCATTATTCAAGGTGATCGT
  ATTCTTCCAGTGAAAACAAGACTGAAATATGATGGCCCAAAATTTATTAAAAAGCTATATTTTCCTGAG
  AGACTGATACATACACTCATACATATATGTGTTCCCCTTTTCCCTGTAATATAAATTACAAATCTGGGC
  TCCTTTGAAGCAACAGGTTGAACCAACAATGATTGGTTGATAGACTAAGGATATATGCAACTCTTCCAG
  ACTTTTCCATAAAGCTCTCTCGGCAGTCGCTCACACTACAATGCACACAAGGATTGAGAAGAGTTAAAG
  GCTAAAGAAAACATCTTTTCTAGCTTCAACAGAGAGGTTTCACCAGCACATTTACCAGAAGAATCTGGG
  AATGGATTCCACTACAGTGATATTGACTGCATCTTTAAGAAGTGACCATTATACTGTGTATATATATAT
  AAACACACACACATATATATATATATATATAGTACTCTAATACTGCAAGAAGGTTTTTTAAACTTCCCA
  CTTTATTTTTTATACACATTAATCAGATATCATTACTTGCTGCAGTTGCAACTATGCACTTGTATAAAG
  CCATAATGTTGGAGTTTATATCACTCATTCCTGTGTACCTGATGGAAGTTGCATGTTCATGTTTAAGCA
  GTTACTGTAACAAGAAGTTTAAAGTTAATTATATCAGTTTCCTAATGCTTCATGATAGGCAACTTTACC
  CATTTTGAATGCCTTAATTTAATTTTTTTCAAAGTCTCAGCCCTGTCTGTATTAAAAAACAAAAAAAGC
  GTTTACCAGCTCTTAGGATGTAAACTAGCTTTGTGGAAGATAAATCGTGCACTATTTTTACACATAAAT
  AGTTATATCAATGTCAGCCTATTTTGATTAACAAATGTTTTTAAAGTATTATTGGTTATAGAAACAATA
  ATGGATGGTGTTGGAACTAATATATCCTTGATGTCTGTCTATTATTCATTCAACTCTTTTTACAGACCT
  CAGTATTAGTCTGTGACTACAAAATATTTTATTTGCTTTAAATTTGCTGGCTACCCTAGATGTGTTTTT
  ATTCCTGGTAAAGACATTTGTGATTACATTTTCACACTTAAGATTCAAAATTTTTCCCAAATATAAAGA
  AAACTAAGACAGACTGTAGATGCATTTTAAATATTTAAATATGATCCTCAGACATGCAGCTGTGTGTGG
  CAGTATTTTAGTACCGGGTTAAGAAAACTGGCAACTGGGAAGAAGTGGCCTCAAAGGCACTTAATTTGA
  TTTTTATTTTTTAAATGCTGTCAAAGTTACAGTTTACGCAGGACATTCTTGCCGTATTCTCATGATCCC
  AGATAAGTGTGTGTTTTATACTGCAACAATATGCAGCAATGGTAAGCGTAAAGTTTTTTTTTTGTTTTT
  GTTTTTTTTTATATTATGAAGTCTTTTAACAGTCTCTCTTTATATAAATACACAGAGTTTGGTATGATA
  TTTAAATACATCATCTGGCCAGGCATGGTGGCTTACGCCTGTAATCCTAGCACTTTGGGAGGCCAAGAC
  GGGCGGATCACCTGAGGTGAGGAGTTCAAGACCAGCCTGCCCAACATAGTGAAACTCCGTCTCTACCAA
  TATACAAAAATTAGCCGGGCATGATGGTGGTGGCCTGTAATCCCAGCTACTTGGGAGGCTGAGACAGGA
  GAATCGCTTGAACCCAGGAGACGGTGGTTGCAGTGAGCGAAGATCGAGCCACTGCACTCCAGCCTGGGC
  AGCTGAACAAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAGTCATCTAATATTTCGATGAAGGAAATA
  GACATTTTTCATTGATTCCCTCTGATGTCAGTTAGAGATATTTAAAAAGTTAGATCCCCATAATAGATA
  CTATTGTGGAAACTGAAGAGTAGCTACTGGCCTACTGATAGACTGAAACTGGTTTTGTTATTCTTAAC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004850.5
• Summary: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]
• Locus ID: 9475
• MW: 78.4