AMPD3 (NM_001025389) Human 3' UTR Clone

CAT#: SC216052

3' UTR clone of adenosine monophosphate deaminase (isoform E) (AMPD3) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AMPD3
• ACCN: NM_001025389
• Insert Size: 1725 bp
• Sequence Data:
  Insert Sequence

  >SC216052 3’UTR clone of NM_001025389
  The sequence shown below is from the reference sequence of NM_001025389. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCAGAAGAGATCACCGCCTTGACCAACTAGGTCCAGCATTTGACATGCATTTTAACTTTTTGGTTCAAT
  TTCAAGTCTGCTGTGGCTAATAGTGGTCAAGATTCCGAACTAGGACTTTCCTCTGTGAAGAGGATGCCT
  CTGAAGAAATTTTAAACTGGTGATTTTGGTTGCACTGCTCACTTTAAGAGTTAACATGCTCACTTGTTA
  GTATTTCTGAGTAACAAGATGGTGACTTCTCCTTGGGGATCTGGGAGCTGAGCACTTGTCTATACTTGT
  TCCTAATTTTCCAAGTATTTCTCTTGAAACTGCCAGTGCCTGAACTGTTGGGGCCAGGATTTTCCCTGG
  TCAGATGCCAAGTAACATGTGGTTTTCTGCCATACTTTTCTCCATTGGCCCAGGTAGGCTAATTGGTAG
  TTGTTCATTTCAGCCTCTGGATGGCTGGCTGCCTTAAACACAATCAATTTCAAAGCTCCATTTCATAAA
  GGGGCTACTTTGAAGGAGTTAAGATGGAAGACTTCCTTCTTGACAAATTGTGTTTTTAGTGAATTTCTT
  AAACCGTTTTATTTAGCCCTCCTTCCCTCTTTCTAGTTGGAAGCCAAATGTACTCATGAAAACAGCCAC
  TCCTATTCTGAGTCTTGGTTTCTTCACCTAGAAAGTGAGGGTTTGGACTAGATGAGTGGCTTTCAGGGT
  GTTCTGTGAATCTCCTCATGAATACTTTAGGGTAGGGGAGGGAAGGGAGTGAGTGATGCTCAGGGGCTG
  TCAAAGTGACTGCGTTCATCAGTTTTACACTGGGGCTGCTACATAATATTTTCATTTGAACGAAGAACT
  TCAAAAAGCACAGGACTAGATGATCTCTGTTCCTTTTGGCTCTAATATGCTACAACTGTAGGCCAATTA
  TCACTTTACCAATTAAGAGTTAGGCCAGATAAGTGAAATTTAACTTAAGGGCACACAGCTAATAAGTAA
  TAGGCCTAAACTGGATTTCCTTATTCCAAATCCTGTCTTTTCCCCACTATTCCATTAGACCCCACAAAT
  GTTAGTTGTGTGTGTGTGTGTGTGTGTTTTTAATCACTGTAACCGGATGCATTTTTTTAAGGCAAAATT
  TCTCCCTTATCTACTATGATGACTTCAGAAGATACAATGGTCCCAGGGGCCAAGTAGAAAGCATTTTTA
  AAGATTAATCTGAATTAAGCTTTATCAGTGTACTCTTTATCTGTGTTACTAGTGCCTGGTATGTAGTAG
  GTGCTCAATAAATGCATATTGAATAACTAAGTGAATTTCTTTTGGCAACTTTTTAAGGACATGTGCTCT
  TAGTACTTAAGAGGCTGCTCAAGGACCTTCCTATCTATTTCTGTGGTCAAATTCAGACTACAGGAGGTC
  TTTTTGAATCATTATAGTTAGAAGAAGAATCCAGTTTCTGCCTGTGAACTTATTTGACTTTAGATTGTC
  TCATCTTGTGACTTTATAATGCCTGTCCCCTCCACTGGTCAATTCAGCATATGGAAGTATAAATGCAGT
  CTTTTTACTAGGCAGATATATATGCTATCTTACAGCTAATTATGAAATTGAATGAAAATCCATTGTTAT
  CTTAGGGATTAGTTTTGAAAAGCCCCCGTTTATATACATTTGCCCTAACATAGGAAGTATTGTGTAGTT
  TCTCATTGTCATTTTATCTGTATCAAGTATCTTTTTTTAAATTGTTAAATAAAGTCAGCTAGGACTGTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001025389.2
• Summary: This gene encodes a member of the AMP deaminase gene family. The encoded protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. This gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
• Locus ID: 272
• MW: 65.3