NPC1L1 (NM_013389) Human 3' UTR Clone

CAT#: SC211176

3' UTR clone of NPC1 (Niemann-Pick disease type C1 gene)-like 1 (NPC1L1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NPC1L1
• Synonyms: LDLCQ7; NPC11L1; SLC65A2
• ACCN: NM_013389
• Insert Size: 942 bp
• Sequence Data:
  Insert Sequence

  >SC211176 3’UTR clone of NM_013389
  The sequence shown below is from the reference sequence of NM_013389. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCTTGCCCAACAATGGGCGGCAGTTCTGATACAGCCAGAGGCCCTGTCTAGGCTCTATGGCCCTGAAC
  CAAAGGGTTATGGGGATCTTCCTTGTGACTGCCCCTTGACACACGCCCTCCTCAAATCCTAGGGGAGGC
  CATTCCCATGAGACTGCCTGTCACTGGAGGATGGCCTGCTCTTGAGGTATCCAGGCAGCACCACTGATG
  GCTCCTCTGCTCCCATAGTGGGTCCCCAGTTTCCAAGTCACCTAGGCCTTGGGCAGTGCCTCCTCCTGG
  GCCTGGGTCTGGAAGTTGGCAGGAACAGACACACTCCATGTTTGTCCCACACTCACTCACTTTCCTAGG
  AGCCCACTTCTCATCCAACTTTTCCCTTCTCAGTTCCTCTCTCGAAAGTCTTAATTCTGTGTCAGTAAG
  TCTTTAACACGTAGCAGTGTCCCTGAGAACACAGACAATGACCACTACCCTGGGTGTGATATCACAGGA
  GGCCAGAGAGAGGCAAAGGCTCAGGCCAAGAGCCAACGCTGTGGGAGGCCGGTCGGCAGCCACTCCCTC
  CAGGGCGCACCTGCAGGTCTGCCATCCACGGCCTTTTCTGGCAAGAGAAGGGCCCAGGAAGGATGCTCT
  CATAAGGCCCAGGAAGGATGCTCTCATAAGCACCTTGGTCATGGATTAGCCCCTCCTGGAAAATGGTGT
  TGGGTTTGGTCTCCAGCTCCAATACTTATTAAGGCTGTTGCTGCCAGTCAAGGCCACCCAGGAGTCTGA
  AGGCTGGGAGCTCTTGGGGCTGGGCTGGTCCTCCCATCTTCACCTCGGGCCTGGATCCCAGGCCTCAAA
  CCAGCCCAACCCGAGCTTTTGGACAGCTCTCCAGAAGCATGAACTGCAGTGGAGATGAAGATCCTGGCT
  CTGTGCTGTGCACATAGGTGTTTAATAAACATTTGTTGGCAGAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_013389.3
• Summary: The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Locus ID: 29881
• MW: 33.8