POFUT1 (NM_172236) Human 3' UTR Clone

CAT#: SC210843

3' UTR clone of protein O-fucosyltransferase 1 (POFUT1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POFUT1
• Synonyms: DDD2; FUT12; O-Fuc-T; O-FucT-1; O-FUT; OFUCT1
• ACCN: NM_172236
• Insert Size: 903 bp
• Sequence Data:
  Insert Sequence

  >SC210843 3’UTR clone of NM_172236
  The sequence shown below is from the reference sequence of NM_172236. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCCTGTGTTACCTTACTCTTCCCAAGGTGAAACTGGAAGCAGCTTCTAGAGAACAGCTTCAGGTGCAGA
  CCGAGAAATGACGTCATCCTGATAATTATTGGACGTGTTTATTAATTGCACCCCATTAGCCCCTGTTTT
  TAAAAAATATAGGTTAGCAGGACGTGGGCACAGGCCGTGAAACATTTATAGGGCGCCTGTCACGTGTGA
  GACATAGTAATCAGAGGCCAATGGTGATGTGTGAAAATGAGGATGACCAGCTCTGGTTTTCTAGGAGCT
  ATGGTCTCATAATGACAATAATCAGCATAGTGTACTTGTCCTTAATGTTATAGCATGGCAGGAAAGACC
  ATGGGCTTAGAAGGCAGACATTGGTTAGGAGGTGCAGGGAACATACTAATTCATGTGCGGTCAAAGCAC
  TTGGCATTTAATAGGGCAAAGGTAAACAGATGTAGAGAAATGAGTCTCATGCCTGTAATCCCAGCACTT
  TGGGAGGCCAAGGTGGGTGGATCACTTGAGCCTAAGAGATCAAGGCTGCAGTGAGCCATGATGGTACCC
  CTGCACTCCAGCCCAGGTTACAGAGCAAGGGCCTGTCTCAGCAAAACAAAACAAGCACAGGCCAAGGAG
  TGAGATAGCCAGCGCTGATGGTATCTGCCTCGTAGTGCATGAAGTGCATGTCATACACTAAGCAGCCAG
  CCAGTGTTGGCTGTCAGTGTTATTACTCACATGCTTTATTTTATTTAAGACTTTGAGATAGTTCATGAA
  GTGGGTATTATTATCAATTTCCATTTTATAGGTGAGGAAACTGAGCCCAGTGAAGTTAAATAACTTCCC
  AAGGTCATTGAGCTGTTGAGTGGACAGCTCAGGTCTGTTTGTAAATCTTTAATAAATAACAACAGCTAA
  GATTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_172236.2
• Summary: This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Locus ID: 23509
• MW: 33.8