PCDH15 (NM_001142770) Human 3' UTR Clone

CAT#: SC209887

3' UTR clone of protocadherin 15 (PCDH15) transcript variant J for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCDH15
• Synonyms: CDHR15; DFNB23; USH1F
• ACCN: NM_001142770
• Insert Size: 1440 bp
• Sequence Data:
  Insert Sequence

  >SC209887 3’UTR clone of NM_001142770
  The sequence shown below is from the reference sequence of NM_001142770. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTAGAGAGCCTAGTGAGGAGGAAGAAGTAGTTGTAACTATCGAAAAACCACCAGCAGCTGAGCCTACAT
  ACACAACATGGAAGAGAGCCAGAATATTCCCCATGATTTTTAAGAAAGTTAGAGGATTAGCTGATAAAA
  GAGGAATCGTTGACCTTGAGGGTGAAGAGTGGCAGAGACGCCTTGAGGAAGAAGATAAAGATTATTTGA
  AACTCACTCTGGACCAAGAGGAAGCAACAGAAAGCACTGTAGAATCAGAGGAGGAATCCTCCAGCGACT
  ATACTGAATACAGTGAAGAAGAGTCTGAGTTCAGTGAGTCTGAGACTACAGAAGAGGAATCTGAGTCAG
  AGACACCCTCTGAGGAGGAGGAGAGTTCCACCCCTGAATCAGAAGAATCGGAATCCACAGAGTCAGAAG
  GAGAAAAAGCAAGGAAAAACATTGTGCTTGCAAGAAGAAGGCCCATGGTTGAGGAGGTCAAGGAAGTCA
  AGGGTAGGAAAGAGGAGCCACAAGAAGAACAAAAAGAACCTAAGATGGAAGAAGAAGAACACTCAGAAG
  AAGAAGAAAGTGGACCAGCCCCTGTGGAAGAAAGTACAGACCCTGAAGCTCAAGATATCCCTGAAGAGG
  GCAGTGCAGAATCAGCTTCGGTGGAAGGAGGTGTGGAAAGTGAGGAGGAATCAGAATCAGGTAGTAGTA
  GCAGTAGTAGCGAAAGTCAGTCTGGAGGTCCATGGGGCTATCAGGTACCAGCGTATGACAGAAGCAAGA
  ATGCAAACCAAAAGAAGTCGCCAGGAGCAAACTCTGAAGGTTACAACACAGCACTTTGAAAGAGATCAC
  AGTAGGTGGAAGTTTTGCTGTGATGAGTGCTCTCGTGTGCAGTGCCTTCTGTGTGTTCTCCAAAGTGAC
  ACTTGAAAGGGAGGAAATTGATCAAGATTTTGATATGACTTATGATCAGAAGCATGCCAGAAAAGTGAA
  TATATATGTTGTCCAAATCCATTTGTATATTAGACTGTATTAGAGTAAAACTGACTAATCCTTCCTATA
  TTAGTAAATCAGAACACATGCAATCAGCTAATCAATATTTAATATTTACTGGTAGGATTTTTGTTAATG
  CAATGTGGCATTATGTATTATCAGTGACACTCTGTCTATTGGAATGGATGGCATATATATATATATATA
  TATATACACACACACACTATATATGTTTTTATATCATCTTTCAAAATAAATACTAAGACAAGCTTCAAC
  AAATTATATCTTTAACAGTCGTCTACTTTTGCATATATGTACGTTTCAACTGGCAAAGGTATTATAAAA
  ACTATCATGAACATGTTCAAATAAGTAAGTTTGTATCTGCAACTTCAAGTATTAGGAATTCAATGAAAT
  AACATTTTTCAATTAATTAAAAGTTATTACAGCTTATTTTCTGTTATTATTAGCTACAGT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001142770.3
• Summary: This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]
• Locus ID: 65217
• MW: 57