VPS4A (NM_013245) Human 3' UTR Clone

CAT#: SC209710

3' UTR clone of vacuolar protein sorting 4 homolog A (S. cerevisiae) (VPS4A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VPS4A
• Synonyms: CIMDAG; SKD1; SKD1A; SKD2; VPS4; VPS4-1
• ACCN: NM_013245
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC209710 3’UTR clone of NM_013245
  The sequence shown below is from the reference sequence of NM_013245. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCTCAGAGGACTTTGGGCAAGAGAGTTAAAAGCTGCTTCACTTGGGCAATGGTGAAGGTGGGAGGTTG
  ATTGGGGCAAATCCAGGCACTCCCCATGTCAACAGCCAGACAGGGCTCCAGGGCTTGTCCCAGTCAATA
  CAGAGTTCCCTCTGCTGTCTGGCCGTCTGCCAGGGAGCCAGAAGGAAGGGCCTTGCAGCCACAGAGACA
  CTCCACTGCCCTGGGGCACACAGTGGACACTGCTCTTCCTACTTCCTCCTCTCCTGGATGCTCATCAGC
  TCCTTCTGCCTCCCCCCCTTTTTTTTCCATCTTTTGTTCCCCTAAATTAATGCTGCTTGGATTTTCATC
  TTATTTATAAAGATAAAATCACCTGGAAGTGTCAAGGAGTGGGGCGGGGTGGCGGGGGAGAAGCAGCCG
  TGCTGCCAGGTCACCCAGACCTCCAGACAGCCGGCTAGCCCCACTGCCCGTTCCTTTTACGCCCAAGTT
  TTGCTCCTTGAGAGCAGATTGGCTGATGCCCCTGCAACCCCAGCCCAAGCTCTGCCTCAAAGACCGAGT
  GACATAAGCCATTCCCACCCTCCTAGGTTCACATCCAGGGCTGTGTCTTCCTTGGGGGAGGAGATGGTG
  TCGTTTAGATCAGGGTAAGGCAGTCAGGCGGGTGTTCACCACTGCCTTTTCTTCCTCTGAGCGTGAGAA
  CACTGAACCCAGCCACTGCCCCTGGGTCCCTGTCCTGGAAATGGTCTAATAAATCCTTTTCCCTTCTTG
  AGCTACCCAAGTGATCTCATCTTTCCTTGACTCTTAACTGAGACTCTGAAGGGCCACCCTTGCTTCCAA
  TTAAAAGACCCTCTGGTTTTCTGTCTCCTCTCTTTTCTAAAGCAGAGCAGGAAACAGAACACTGCAGTT
  CTTAGGCCTGTTCTCCAGTCAGGTGTGCAAGGCCTCCTCCCCTTAAGGCCTCAGAAGTTTGGCTGGGGA
  TGTTTGTGGGATCCAGACAGTTCTTGCCGTTGTTCGGCCTACAGATCAGAGACTGCAAAGTGGGAGCCC
  TGCGTACCAGACCCGTCCTGCAGCTGTGTTTGTTTGGTTTGGTCTGCCGCTAACATTTAAAAGTCGAGA
  GTTGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGCCGCTAACATTTAAAAGTCGAGAG
  TTGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGACGCGGGCGGATCACAAGG
  TCAGGAAATCGAGAACATCCTGGCTAACACAGTGAAACCCCGTCTCACTAAAAATAAAAAAATTAGCCG
  GGCGTGGTGGTGGGCGCCTGCAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGG
  GGGACGGAGCTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTCTG
  TCTCAAAAAAAAAAAAAAAGTCGAGAGTTGACATAAAAATTCAGAATTCTGACTTCTAGAAGATTAAAT
  TAGGTAATGTTGGGCCTGCGTCCTCATGGGGCAGCCTGTCAGCGGCCACTAAGTAGAGGCTGCTCTGTA
  CAGAAGGGGTCCCCCATCCCCACTCATCTGTGTTGCCTGCCCAGATAGCCCCATGGCCATTTCAGTTTG
  CGACCCCCAACAGACCAAGGACCCAGAGCTGGCAGCCCTGGACCCACGTCCGGCAGGCAGTGAAGCTGC
  AGAGGCTGTCCCACGTTTACCCCATGCATATAAACAGACCAAAGTCAAAGCACATTCACACAGAAAGAC
  CTGGCCTAGTAAATGAAGCTTATAGCCTGCAAAGGGCTTTGTTCCAACCTTCCCTCTCCAAAACCCAGT
  ACTGCTCCTCTACCTTTCAAACCTGGTTGGGCCAGAACTGATTACATGGCCAACTCTTGGCTCACACTG
  GGCTTGCACTGGGCCATTTCTTTGCTCTGGTTCAGAGCCTCTGCAGAAGTGAGCTAACTTTGGATCAG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_013245.3
• Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008]
• Locus ID: 27183
• MW: 72.4