GABA B Receptor 2 (GABBR2) Rabbit Polyclonal Antibody

CAT#: TA328675

Rabbit Polyclonal Anti-GABA (B) R2

Product Images

Western blot analysis of rat brain membrane: 1. Anti-GABA(B) R2 antibody, (1:200). 2. Anti-GABA(B) R2 antibody preincubated with the control peptide antigen.

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  Expression of GABA(B) R2 in mouse hippocampus. Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 antibody, (1:100). A. GABA(B) R2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABA(B) R2 in layers, but not in pyramidal neurons.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABA(B) R2. Intracellular, C-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
• Reconstitution Method: Add 50 ul double distilled water (DDW) to the lyophilized powder.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: gamma-aminobutyric acid type B receptor subunit 2
• Database Link:
  NP_005449
  Entrez Gene 83633 RatEntrez Gene 242425 MouseEntrez Gene 9568 Human
  UniProt ID O75899
• Background: GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA (A) R and GABA (C) R, and the metabotropic GABA (B) receptor, [GABA (B) R].The latter belongs to the G-protein-coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord.The functional GABA (B) receptor [GABA (B)R] is a heterodimer consisting of two subunits, the GABA (B) R1 and the GABA (B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.GABA (B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA (B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA (B) R2 is highly expressed in brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in testis.
• Synonyms: GABABR2; GPR51; GPRC3B; HG20; HRIHFB2099

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction