RFX5 Rabbit Polyclonal Antibody

CAT#: TA319308

Rabbit polyclonal anti-RFX5 antibody

Product Images

Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2, 500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band (s): none.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:5,000 - 1:25,000, WB: 1:1,000, ChIP: User Optimized, Gel Shift: 0.5 uL to 1.0 uL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: regulatory factor X5
• Database Link:
  NP_000440
  Entrez Gene 5993 Human
  UniProt ID P48382
• Synonyms: 5; 5 (influences HLA class II expression); OTTHUMP00000082795; OTTHUMP00000196318; regulatory factor X
• Note: Anti-RFX5 is specific for the RFX5 protien. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA.

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation, Primary immunodeficiency