siRNA (27-mer): A Potent Mechanism for Gene Silencing

Trilencer-27 siRNA kits contain Dicer-Substrate duplexes that provide two critical improvements over the use of traditional 21mer siRNA designs. It takes advantage of the natural processing by Dicer to produce 10-fold higher potency and specificity than shorter 21-mer siRNA forms. The 27-mer duplexes also evade mammalian interferon response when expressed in mammalian cells and initiate strong and specific gene silencing. Learn more about Dicer-Substrate technology in this Nature Publication.

27mer siRNA

Key Features and Benefits

Genome wide coverage against human, mouse and rat genes: view citations
27mer Dicer-substrate duplex : higher potency & minimal interferon response
Guarantee statement:

OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA, when the following are true:

  • The dicer-substrate duplexes are used at 10 nM concentration by quantitative RT-PCR
  • The TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected
  • The HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.

For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Support at Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

Each of our siRNA products come as a kit. It contains 3 unique 27mer siRNA duplexes (2 nmol each), one negative control and one buffer. Each gene specific siRNA is labeled as the kit catalog number plus a/b/c. For example:

Kit Catalog Kit Component
  • 3 gene specific siRNAs : SRxxxxxxA, SRxxxxxxB, SRxxxxxxC
  • SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
  • SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml

Bulk Order Options

If you purchased our kits and already know which one works best for your gene, you can order this single siRNA in bulk by submitting a bulk request form. Each 10 nmol of single siRNA duplex is charged for $375. Delivery time is around 2 weeks.

Additional Products You May Need

SKU Description Availability Data Sheet
SR30002 Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol In Stock
SR30003 Trilencer-27 HPRT Positive control siRNA duplex - 1 nmol In Stock
SR30004 Trilencer-27 Universal scrambled negative control siRNA duplex - 2 nmol In Stock
SR30005 RNAse free siRNA duplex re-suspension buffer (2ml) In Stock
TT320001 SiTran2.0: Transfection reagent designed for RNAi duplex (0.5 ml) In Stock

Related Products and Services

Recent Citations using our siRNA Oligo Duplexes

1. The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension

Published in Communications Biology, 2023 Aug, using SR30003
PubMed: 37620393

2. Mitochondrial cytochrome P450 1B1 is involved in pregnenolone synthesis in human brain cells

Published in The Journal of Biological Chemistry, 2023 Jul, using SR301124
PubMed: 37442234

3. AQP3-Dependent PI3K/Akt Modulation in Breast Cancer Cells.

Published in International journal of molecular sciences, 2023 Jun, using SR300258
PubMed: 37175840

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