Antibodies

Sheep/Goat IgG donkey polyclonal antibody, HRP

Applications ELISA: 1/12800-1/25600.
Western Blot: 1/20000.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections: 1/50-1/100.
Reactivities Goat, Sheep
Conjugation HRP

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. 
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Reactivities Mouse

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/200) and Flow Cytometry (1/100-1/200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE

Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Goat
Conjugation FITC

Sheep IgG (H+L chain), adsorbed F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry: the maximum amount of reagent required to stain 1x10e6 cells is approx. 1.0 µg of antibody conjugate.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Conjugation PE

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
Immunofluorescence: 1/100-1/200.
Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Rabbit
Conjugation PE

Rat IgG (H+L chain) F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:200) and Flow Cytometry (1:100-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation PE

Human IgG (adsorbed F(ab)2 Fragment) donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Conjugation PE

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:8,000 to 1:36,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation Texas Red

Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions for ELISA: 1:10,000-1:50,000.