Primary Antibodies

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Acpp (Pain System Marker) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Mouse
Conjugation Unconjugated
Immunogen Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system.
Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.

PRNP chicken polyclonal antibody, Purified

Applications IF, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% Sodium Azide.

Amyloid beta A4 protein / APP (501-522) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Chickens were immunized with synthetic peptide APP#4 conjugated to keyhole limpet hemocyanin (KLH).  The sequence of APP peptide #4 is EQK DRQ HTL KHF EHV RMV DPK K, which corresponds to residues #501-522 of the Human gene product (NP_000475.1)].  
Identical sequences are also found in the Mouse and Rat homologs of APP.

Amyloid beta A4 protein / APP (556-575) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Chickens were immunized with synthetic peptide APP#5 conjugated to keyhole limpet hemocyanin (KLH).  The sequence of APP peptide #5 is EEI QDE VDE LLQ KEQ NYS DD, which corresponds to residues #556-575 of the Human gene product (NP_000475.1)]. 
Identical sequences are also found in the Mouse and Rat homologs of APP.

Casein Kinase 1 alpha (CSNK1A1) chicken polyclonal antibody

Applications IF, WB
Reactivities Human
Conjugation Unconjugated