pCMV6-AC-FC-S Mammalian Expression Vector

CAT#: PS100054

pCMV6-AC-FC-S, mammalian vector with C-terminal FC and secretion signal, 10ug

Product Images

• 

Specifications

Product Data

• C-Tag: FC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Features:
  • ORFs cloned in this vector will be expressed in mammalian cells as a secreted tagged protein with a C-terminal murine Fc tag. Due to the presence of an N-terminal secretion signal sequence, the fusion protein will be secreted and this facilitates the downstream purification
  • The Fc-tag encodes the CH2 and CH3 domains of the mouse IgG heavy chain and the hinge region. A fusion protein is comprised of two relatively independent parts connected with a flexible spacer. Fc fusion provides several advantages in protein applications:
    1. Easy purification using protein A or protein G affinity chromatography.
    2. Increased solubility.
    3. Excellent antigen in mouse mAB production without cleavage, as the murine Fc is not antigenic in mice.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal Fc-tagging vector, PS100056
  • Validation Data
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • FC_R Primer can be used to sequence the target sequence after cloning.
• Download Vector Sequence:
• Schematic of the multiple cloning sites: