Protein A (FITC conjugated) Staphylococcus aureus Protein
Specifications
| Product Data | |
| Species | Staphylococcus aureus |
|---|---|
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | This product was prepared from chromatographically pure Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A. |
| Conjugation | FITC |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 5.0 mL |
| Preparation | Lyophilized purified Ig fraction. |
| Recommended Dilution | FLISA,Flow Cytometry,IF Microscopy |
| Protein Description | Fluorescein Conjugated Protein A. |
| Note | Protein A Fluorescein Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Reference Data | |
| UniProt ID | P38507 |
| Synonyms | ProA, Staphylococcus A protein, Protein A FITC |
| Summary | Protein A is a 56 kDa cell wall protein originated from Staphylococcus aureus. It contains five homologous Ig-binding domains. Each domain is able to bind the Fc fragment of a wide range of mammalian immunoglobulins. Protein A affinity to Fc may vary in dependence of IgG species origins or subclasses. Protein A is often coupled to solid matrixes such as Sepharose™, magnetic particles or latex for IgG purification from ascites, serum or hybridoma culture media. Protein A is often coupled with reporter molecules such as a fluorescent dye, DyLight™, enzymes, biotin, or other detection agents. |
| Protein Families | 000) as well as other antibody based fluorescent assays., 000) Flow Cytometry/FACS (1/1, 000-1/5, Immunofluorescence Microscopy (1/1 |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Neuraminidase-associated plasminogen recruitment enables systemic spread of natural avian Influenza viruses H3N1
Schön, Breithaupt, Höper et al
PLoS Pathog (2021) 17 (4), e1009490 DOI: 10.1371/journal.ppat.1009490
Schön, Breithaupt, Höper et al
PLoS Pathog (2021) 17 (4), e1009490 DOI: 10.1371/journal.ppat.1009490
Cited by 13
AI Summary: OriGene’s goat-anti-chicken-IgY-FITC secondary antibody was used to detect chicken antibodies in cell-based immunofluorescence assays, enabling visualization of viral antigen-antibody interactions in the study.
Snippet: r 1 hour of incubation we washed the cells three times with washing buffer (0,025% Tween-20/PBS), before adding the fluorescein isothiocyanate (FITC) labeled secondary Ab (goat-anti-chicken-IgY-FITC (OriGene Technologies GmbH, Maryland, USA)), diluted 1:400 in blocking buffer. Following 1-hour incubation, the cells were washed three times with washing buffer and finally 20% glycerol in PBS was add...
The Role of Human Platelet Preparation for Toll-Like Receptors 2 and 4 Related Platelet Responsiveness
Koessler, Niklaus, Weber et al
TH Open (2019) 3 (2), e94-e102 DOI: 10.1055/s-0039-1685495
Koessler, Niklaus, Weber et al
TH Open (2019) 3 (2), e94-e102 DOI: 10.1055/s-0039-1685495
Cited by 17
AI Summary: OriGene products, specifically the TLR2 antibody (Cat# TA302122) and TLR4 antibody (Cat# TA300665), were used in this study to evaluate the expression levels of TLR2 and TLR4 on platelets by flow cytometry, contributing to the assessment of how platelet preparation methods affect TLR-mediated platelet responsiveness.
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
