LRPAP1 / A2MRAP (His-tagged, myc-tagged) Rat Protein
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Specifications
Product Data | |
Species | Rat |
Expression Host | E. coli |
Tag | His-tagged, myc-tagged |
Predicted MW | 40 kDa |
Concentration | lot specific |
Purity | >95% determined by SDS gelelectrophoresis |
Presentation | Purified |
Buffer | Presentation State: Purified State: Lyophilised purified protein Buffer System: TBS, pH 7.5, 0.1% BSA, 0.09% NaN3 |
Reconstitution | Restore with 100 µl (100µg), 250 µl (250µg) or 500 µl (500µg) distilled water |
Preparation | Lyophilised purified protein |
Applications | Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoblotting. Receptor-binding studies. |
Protein Description | Rat his:RAP:c-myc fusion protein |
Note | Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (cf. Bajari et al. 2005, see ref. below). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+). |
Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at -20°C for one month or (in aliquots) at -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data | |
RefSeq | NP_001162584 |
Locus ID | 116565 |
UniProt ID | Q99068 |
Cytogenetics | 14q21 |
Summary | Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.[UniProtKB/Swiss-Prot Function] |
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