Interleukin-4 / IL4 (25-153, His-tag) Human Protein

CAT#: AR09136PU-N

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Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.


Product Datasheet for 'AR09136PU-N'


USD 590.00


Availability*
2 Weeks

Size
    • 50 ug


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Specifications

Product Data
Description Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Tag His-tag
Predicted MW 17.2 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 95 % pure by SDS - PAGE
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Bioactivity The ED50 for this effect is less or equal to 0.5 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Endotoxin < 1.0 EU per 1 ug of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000580
Locus ID 3565
Cytogenetics 5q31.1
Synonyms BCGF-1; BCGF1; BSF-1; BSF1; IL-4
Summary The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway

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