Interleukin-4 / IL4 (25-153, His-tag) Human Protein
Interleukin-4 / IL4 (25-153, His-tag) human recombinant protein, 50 µg
|Description||Interleukin-4 / IL4 (25-153, His-tag) human recombinant protein, 50 µg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS|
|Predicted MW||17.2 kDa|
|Purity||>95% pure by SDS - PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
|Bioactivity||Biological: The ED50 for this effect is less or equal to 0.5 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.|
|Endotoxin||< 1.0 EU per 1 ug of protein (determined by LAL method)|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.|
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||BCGF-1; BCGF1; BSF-1; BSF1; IL-4|
|Summary||The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.