Glucokinase (GCK) (NM_000162) Human Over-expression Lysate

CAT#: LC400059

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GCK HEK293T cell transient overexpression lysate (as WB positive control)


 Product Datasheet for 'LC400059'

  View other "GCK" proteins (15)

USD 95.00


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Size
    • 20 ug


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Specifications

Product Data
Description GCK HEK293T cell transient overexpression lysate (as WB positive control)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200472
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
Accession Number NM_000162, NP_000153
Other Names FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
Predicted MW 52.2 kDa
Components 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
RefSeq NP_000153
Locus ID 2645
Cytogenetics 7p13
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones