Cytokeratin 8 Bovine Protein

CAT#: BA1007S

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Cytokeratin 8 bovine protein, 0.1 mg



USD 280.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description Cytokeratin 8 bovine protein, 0.1 mg
Species Bovine
Protein Source Liver
Predicted MW 52 kDa
Concentration lot specific
Purity >95% (determined by SDS gelelectrophoresis)
Buffer Presentation State: Purified
State: Lyophilized
Buffer System: 30mM Tris/HCI pH 8, 9M Urea, 2mM DTT, 2mM EDTA, 10mM Methylammonium Chloride.
Reconstitution Restore with distilled water.
BA1007S: 70 µl (final volume 100 µl).
BA1007 : 175 µl (final volume 250 µl).
Preparation Lyophilized
Applications Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For Immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
See References 2 and 3 for more details.
Protein Description Bovine keratin K8 (formerly also designated Cytokeratin 8).
Note Isoelectric Point: pI 6.4
Storage Store at 2-8°C (lyophilized) and at -20°C (reconstituted).
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001243211
Locus ID 3856
Cytogenetics 12q13.13
Synonyms CARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Summary This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Protein Families Druggable Genome

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