MKKS (NM_170784) Human Untagged Clone

CAT#: SC324180

MKKS (untagged)-Human McKusick-Kaufman syndrome (MKKS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MKKS
• Synonyms: BBS6; HMCS; KMS; MKS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_170784.1 TGGCGGCCTGGGGCGGCGCGACGGCTGGTGCGCAGGTACACTGATGCTGAAGTACTATGA
  GCCTTCGGAACTTGTGGAGAGACTACAAAGTTTTGGTTGTTATGGTCCCTTTAGTTGGGC
  TCATACATTTGGGGTGGTACAGAATCAAAAGCAGCCCTGTTTTCCAAATACCTAAAAACG
  ACGACATTCCTGAGCAAGATAGTCTGGGACTTTCAAATCTTCAGAAGAGCCAAATCCAGG
  GGAAGTAGCAGGCTTGCAATCTTCAGGTAAAGAAGCAGCTTTGAATCTGAGCTTCATATC
  GAAAGAAGAGATGAAAAATACCAGTTGGATTAGAAAGAACTGGCTTCTTGTAGCTGGGAT
  ATCTTTCATAGGTGTCCATCTTGGAACATACTTTTTGCAGAGGTCTGCAAAGCAGTCTGT
  AAAATTTCAGTCTCAAAGCAAACAAAAGAGTATTGAAGAGTGAAGTAAAATAAATATTTG
  GAATTACTAATTTGTCATTAAATCATTCTATGCTGATTAGCTTCATAAACATTGAACTTT
  TTGATTTTATAGCCACAATGCTGCATATTCATACTTTAATTCCTAAAGAATAATTTTTAA
  TGTTAAAACGTGATAATGCAATAAATAGAAAAATGTGGTTTACAAAATAAAAACGGTCTT
  CACTAGTTACCACCTGAAGTAAGATGTCTCGTTTGGAAGCTAAGAAGCCATCATTGTGTA
  AGAGTGAACCACTGACAACTGAGAGAGTCAGGACCACACTTTCTGTCTTGAAAAGAATTG
  TAACATCATGCTATGGCCCTTCAGGTAGGCTGAAGCAGCTGCACAATGGCTTTGGAGGTT
  ACGTGTGTACAACCTCACAGTCCTCAGCTCTGCTCAGTCACCTTTTGGTCACACATCCCA
  TTTTAAAGATCCTGACAGCCTCCATACAGAATCATGTGTCAAGCTTCAGTGATTGTGGCT
  TATTCACAGCTATTCTTTGCTGCAACCTGATTGAAAATGTTCAGAGATTAGGCTTGACAC
  CCACCACTGTCATTAGATTAAATAAACATCTTTTGAGTCTTTGCATCAGTTATCTCAAGT
  CTGAGACCTGTGGTTGTCGAATCCCAGTGGACTTTAGTAGTACTCAGATCCTCCTTTGTT
  TGGTGCGTAGTATATTAACAAGTAAACCTGCCTGTATGCTCACCAGAAAGGAAACAGAGC
  ATGTCAGTGCTTTGATTCTGAGAGCCTTTTTGCTTACAATTCCAGAAAATGCTGAAGGCC
  ACATCATTTTAGGAAAGAGTTTAATTGTACCTTTAAAAGGTCAAAGAGTTATAGATTCCA
  CTGTATTACCTGGGATACTCATTGAAATGTCAGAAGTTCAATTAATGAGGCTATTACCTA
  TCAAAAAATCAACTGCCCTCAAGGTGGCACTCTTTTGTACAACTTTATCCGGAGACACTT
  CTGACACTGGAGAAGGAACTGTGGTGGTCAGTTATGGGGTTTCTCTTGAAAATGCAGTCT
  TGGACCAGCTGCTTAACCTAGGAAGGCAGCTAATCAGTGACCACGTAGATCTTGTCCTGT
  GCCAAAAAGTTATACATCCATCTTTGAAGCAGTTTCTCAATATGCATCGTATTATTGCCA
  TAGACAGAATTGGAGTGACTCTGATGGAACCCCTGACTAAAATGACAGGAACACAGCCTA
  TTGGATCCCTAGGCTCAATATGTCCTAATAGTTATGGAAGTGTGAAAGATGTGTGCACTG
  CAAAATTTGGCTCCAAACATTTTTTTCATCTTATTCCTAATGAAGCAACAATCTGCAGCT
  TGCTTCTCTGCAACAGAAATGACACTGCCTGGGATGAGCTGAAGCTCACGTGTCAGACGG
  CACTGCATGTCCTGCAGTTAACACTCAAGGAACCATGGGCTTTGTTGGGAGGTGGCTGTA
  CTGAAACTCATTTGGCTGCATATATCAGACACAAGACTCACAACGACCCAGAAAGCATTC
  TCAAAGATGATGAATGTACTCAAACAGAACTTCAATTAATTGCTGAAGCATTTTGCAGTG
  CCCTAGAATCTGTTGTTGGCTCTTTAGAACATGATGGAGGTGAAATTCTCACTGACATGA
  AGTATGGACACCTTTGGTCAGTTCAGGCAGATTCTCCCTGTGTTGCTAACTGGCCAGATT
  TGCTTTCACAGTGTGGCTGTGGATTATACAATAGCCAGGAAGAACTCAACTGGTCTTTCT
  TAAGAAGCACATGTCGTCCATTTGTGCCACAAAGCTGCCTTCCACATGAAGCTGTGGTCT
  CAGCCAGCAACCTGACCTTGGACTGTTTGACTGCAAAGCTTAGTGGCCTACAGGTGGCTG
  TAGAGACAGCCAATTTGATTTTGGATCTTTCATATGTTATTGAAGATAAAAACTAAGAGA
  ATAGCATGTTCGTATTACAAGAGAAACAAATAAACTAGTCTGTTGGCAATTGAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_170784
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170784.1, NP_740754.1
• RefSeq Size: 2540 bp
• RefSeq ORF: 1713 bp
• Locus ID: 8195
• UniProt ID: Q9NPJ1
• Cytogenetics: 20p12.2
• Gene Summary: This gene encodes a protein which shares sequence similarity with other members of the type II chaperonin family. The encoded protein is a centrosome-shuttling protein and plays an important role in cytokinesis. This protein also interacts with other type II chaperonin members to form a complex known as the BBSome, which involves ciliary membrane biogenesis. This protein is encoded by a downstream open reading frame (dORF). Several upstream open reading frames (uORFs) have been identified, which repress the translation of the dORF, and two of which can encode small mitochondrial membrane proteins. Mutations in this gene have been observed in patients with Bardet-Biedl syndrome type 6, also known as McKusick-Kaufman syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.