MKKS (NM_170784) Human Untagged Clone

CAT#: SC124427

MKKS (untagged)-Human McKusick-Kaufman syndrome (MKKS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MKKS
• Synonyms: BBS6; HMCS; KMS; MKS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124427 sequence for NM_170784 edited (data generated by NextGen Sequencing)
  ATGTCTCGTTTGGAAGCTAAGAAGCCATCATTGTGTAAGAGTGAACCACTGACAACTGAG
  AGAGTCAGGACCACACTTTCTGTCTTGAAAAGAATTGTAACATCATGCTATGGCCCCTCA
  GGTAGGCTGAAGCAGCTGCACAATGGCTTTGGAGGTTACGTGTGTACAACCTCACAGTCC
  TCAGCTCTGCTCAGTCACCTTTTGGTCACACATCCCATTTTAAAGATCCTGACAGCCTCC
  ATACAGAATCATGTGTCAAGCTTCAGTGATTGTGGCTTATTCACAGCTATTCTTTGCTGC
  AACCTGATTGAAAATGTTCAGAGATTAGGCTTGACACCCACCACTGTCATTAGATTAAAT
  AAACATCTTTTGAGTCTTTGCATCAGTTATCTCAAGTCTGAGACCTGTGGTTGTCGAATC
  CCAGTGGACTTTAGTAGTACTCAGATCCTCCTTTGTTTGGTGCGTAGTATATTAACAAGT
  AAACCTGCCTGTATGCTCACCAGAAAGGAAACAGAGCATGTCAGTGCTTTGATCCTGAGA
  GCCTTTTTGCTTACAATTCCAGAAAATGCTGAAGGCCACATCATTTTAGGAAAGAGTTTA
  ATTGTACCTTTAAAAGGTCAAAGAGTTATAGATTCCACTGTATTACCTGGGATACTCATT
  GAAATGTCAGAAGTTCAATTAATGAGGCTATTACCTATCAAAAAATCAACTGCCCTCAAG
  GTGGCACTCTTTTGTACAACTTTATCCGGAGACACTTCTGACACTGGAGAAGGAACTGTG
  GTGGTCAGTTATGGGGTTTCTCTTGAAAATGCAGTCTTGGACCAGCTGCTTAACCTAGGA
  AGGCAGCTAATCAGTGACCACGTAGATCTTGTCCTGTGCCAAAAAGTTATACATCCATCT
  TTGAAGCAGTTTCTCAATATGCATCGTATTATTGCCATAGACAGAATTGGAGTGACTCTG
  ATGGAACCCCTGACTAAAATGACAGGAACACAGCCTATTGGATCCCTAGGCTCAATATGT
  CCTAATAGTTATGGAAGTGTGAAAGATGTGTGCACTGCAAAATTTGGCTCCAAACATTTT
  TTTCATCTTATTCCTAATGAAGCAACAATCTGCAGCTTGCTTCTCTGCAACAGAAATGAC
  ACTGCCTGGGATGAGCTGAAGCTCACGTGTCAGACGGCACTGCATGTCCTGCAGTTAACA
  CTCAAGGAACCATGGGCTTTGTTGGGAGGTGGCTGTACTGAAACTCATTTGGCTGCATAT
  ATCAGACACAAGACTCACAACGACCCAGAAAGCATTCTCAAAGATGATGAATGTACTCAA
  ACAGAACTTCAATTAATTGCTGAAGCATTTTGCAGTGCCCTAGAATCTGTTGTTGGCTCT
  TTAGAACATGATGGAGGTGAAATTCTCACTGACATGAAGTATGGACACCTTTGGTCAGTT
  CAGGCAGATTCTCCCTGTGTTGCTAACTGGCCAGATTTGCTTTCACAGTGTGGCTGTGGA
  TTATACAATAGCCAGGAAGAACTCAACTGGTCTTTCTTAAGAAGCACACGTCGTCCATTT
  GTGCCACAAAGCTGCCTTCCACATGAAGCTGTGGGCTCAGCCAGCAACCTGACCTTGGAC
  TGTTTGACTGCAAAGCTTAGTGGCCTACAGGTGGCTGTAGAGACAGCCAATTTGATTTTG
  GATCTTTCATATGTTATTGAAGATAAAAACTAA
  
  Clone variation with respect to NM_170784.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170784 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCTTTAGTTGGGCTCATACA
  TTTGGGGTGGTACAGAATCAAAAGCAGCCCTGTTTTCCAAATACCTAAAAACGACGACAT
  TCCTGAGCAAGATAGTCTGGGACTTTCAAATCTTCAGAAGAGCCAAATCCAGGGGAAGTA
  GCAGGCTTGCAATCTTCAGGTAAAGAAGCAGCTTTGAATCTGAGCTTCATATCGAAAGAA
  GAGATGAAAAATACCAGTTGGATTAGAAAGAACTGGCTTCTTGTAGCTGGGATATCTTTC
  ATAGGTGTCCATCTTGGAACATACTTTTTGCAGAGGTCTGCAAAGCAGTCTGTAAAATTT
  CAGTCTCAAAGCAAACAAAAGAGTATTGAAGAGTGAAGTAAAATAAATATTTGGAATTAC
  TAATTTGTCATTAAATCATTCTATGCTGATTAGCTTCATAAACATTGAACTTTTTGATTT
  TATAGCCACAATGCTGCATATTCATACTTTAATTCCTAAAGAATAATTTTTAATGTTAAA
  ACGTGATAATGCAATAAATAGAAAAATGTGGTTTACAAAATAAAAACGGTCTTCACTAGT
  TACCACCTGAAGTAAGATGTCTCGTTTGGAAGCTAAGAAGCCATCATTGTGTAAGAGTGA
  ACCACTGACAACTGAGAGAGTCAGGACCACACTTTCTGTCTTGAAAGAATTGTACATCAT
  GCTATGGCCCCCTCAGTANGCTGAAGCAGCTGCACAATGGCTNTGGAGGNTACGTGTGTA
  CAACCTCACAGTCCTCAGCTCTGCTCAGTCACCTTTTGGTCACACATCCCATTNTAAGAT
  CCTGACAGCCTNCTACAGATNATGTGTCAAGCTCAGTGATGTGGCTTATTACAGCTATTC
  TTGCTGCAACTGATGAAAATGTANNAGATAGCTTGCACCCACACTGTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_170784 unedited
  GCATGGCGCAGGGTCACAGGGATGCCACTCTGGGATCTGTTCAGGNAAACAGCTATGACC
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCANTTGCCAACAGACTAGTT
  TATTTGTTTCTCTTTTTTTNCGANCNTGCTATTCTCTTAGTTTAAAAAAAAAAAAAAATT
  NGGGTGGNNCTTCCGCGCTTTTGATTTTTTTTAGAGAAAAAAAAATTTTTTTTTTCCCNG
  CCGCCTTTTTTTTTGGGGGGGGTTGTTTCCCCCCCCCCGGGGCCCCCCCCCCCCCGCGGC
  CGCCCCCCCCCCCCCCCCGCGGTGCGCCGGCGCCCCGGGCTCCCCCCCCCCCCGCCCCCG
  CCCGTTCGCCGCCTCGCCCCCGCCCCCCGCCCGCCGCCGCGCCCCGCCCCCCGGCCCCCC
  CCGTCCCCCCGCCCCCCCTGGGCCTCCCTGTCCTTTCTCGCCGTCGCCGCGGTCCGCCTC
  CCCCTTGCCCCCCCCCCCCCCGCCCGCGCGCCCCGGCCCGCCGCCCCCGCGGCCCCGCGC
  GGTCGTCCGCCCGCCCTGCCCCCCCCCCGCCCCCCCCCCCCGCCCTTCCCGCCGCGCGGG
  CCGTCGCCGCGGTGGTGCCCCGCGCCCCCCGCTCCGCTCCCCCGCGCCCGCCCCCCCCCC
  TCGCCCCGCGCCCCCCCCCCGCCCGCGCCGCCGCCCGGTCTCCGCCCCCCCCCCCTGCCC
  CCCTGCCGGGTCCTCCCCCCCGCGTCCCGTGCGCTTTTCCCCCCGCGCTCCCTTGTGCGT
  CCGCCCCCCGCCGCCGGCGCCACACACCGAAAAAAAACCCCCCACCTCGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_170784
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170784.1, NP_740754.1
• RefSeq Size: 2540 bp
• RefSeq ORF: 1713 bp
• Locus ID: 8195
• UniProt ID: Q9NPJ1
• Cytogenetics: 20p12.2
• Gene Summary: This gene encodes a protein which shares sequence similarity with other members of the type II chaperonin family. The encoded protein is a centrosome-shuttling protein and plays an important role in cytokinesis. This protein also interacts with other type II chaperonin members to form a complex known as the BBSome, which involves ciliary membrane biogenesis. This protein is encoded by a downstream open reading frame (dORF). Several upstream open reading frames (uORFs) have been identified, which repress the translation of the dORF, and two of which can encode small mitochondrial membrane proteins. Mutations in this gene have been observed in patients with Bardet-Biedl syndrome type 6, also known as McKusick-Kaufman syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.