MKKS (NM_170784) Human Tagged ORF Clone

CAT#: RC205742

MKKS (Myc-DDK-tagged)-Human McKusick-Kaufman syndrome (MKKS), transcript variant 2

Product Images

Circular map for RC205742

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MKKS
• Synonyms: BBS6; HMCS; KMS; MKS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205742 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGTTTGGAAGCTAAGAAGCCATCATTGTGTAAGAGTGAACCACTGACAACTGAGAGAGTCAGGA
  CCACACTTTCTGTCTTGAAAAGAATTGTAACATCATGCTATGGCCCTTCAGGTAGGCTGAAGCAGCTGCA
  CAATGGCTTTGGAGGTTACGTGTGTACAACCTCACAGTCCTCAGCTCTGCTCAGTCACCTTTTGGTCACA
  CATCCCATTTTAAAGATCCTGACAGCCTCCATACAGAATCATGTGTCAAGCTTCAGTGATTGTGGCTTAT
  TCACAGCTATTCTTTGCTGCAACCTGATTGAAAATGTTCAGAGATTAGGCTTGACACCCACCACTGTCAT
  TAGATTAAATAAACATCTTTTGAGTCTTTGCATCAGTTATCTCAAGTCTGAGACCTGTGGTTGTCGAATC
  CCAGTGGACTTTAGTAGTACTCAGATCCTCCTTTGTTTGGTGCGTAGTATATTAACAAGTAAACCTGCCT
  GTATGCTCACCAGAAAGGAAACAGAGCATGTCAGTGCTTTGATTCTGAGAGCCTTTTTGCTTACAATTCC
  AGAAAATGCTGAAGGCCACATCATTTTAGGAAAGAGTTTAATTGTACCTTTAAAAGGTCAAAGAGTTATA
  GATTCCACTGTATTACCTGGGATACTCATTGAAATGTCAGAAGTTCAATTAATGAGGCTATTACCTATCA
  AAAAATCAACTGCCCTCAAGGTGGCACTCTTTTGTACAACTTTATCCGGAGACACTTCTGACACTGGAGA
  AGGAACTGTGGTGGTCAGTTATGGGGTTTCTCTTGAAAATGCAGTCTTGGACCAGCTGCTTAACCTAGGA
  AGGCAGCTAATCAGTGACCACGTAGATCTTGTCCTGTGCCAAAAAGTTATACATCCATCTTTGAAGCAGT
  TTCTCAATATGCATCGTATTATTGCCATAGACAGAATTGGAGTGACTCTGATGGAACCCCTGACTAAAAT
  GACAGGAACACAGCCTATTGGATCCCTAGGCTCAATATGTCCTAATAGTTATGGAAGTGTGAAAGATGTG
  TGCACTGCAAAATTTGGCTCCAAACATTTTTTTCATCTTATTCCTAATGAAGCAACAATCTGCAGCTTGC
  TTCTCTGCAACAGAAATGACACTGCCTGGGATGAGCTGAAGCTCACGTGTCAGACGGCACTGCATGTCCT
  GCAGTTAACACTCAAGGAACCATGGGCTTTGTTGGGAGGTGGCTGTACTGAAACTCATTTGGCTGCATAT
  ATCAGACACAAGACTCACAACGACCCAGAAAGCATTCTCAAAGATGATGAATGTACTCAAACAGAACTTC
  AATTAATTGCTGAAGCATTTTGCAGTGCCCTAGAATCTGTTGTTGGCTCTTTAGAACATGATGGAGGTGA
  AATTCTCACTGACATGAAGTATGGACACCTTTGGTCAGTTCAGGCAGATTCTCCCTGTGTTGCTAACTGG
  CCAGATTTGCTTTCACAGTGTGGCTGTGGATTATACAATAGCCAGGAAGAACTCAACTGGTCTTTCTTAA
  GAAGCACATGTCGTCCATTTGTGCCACAAAGCTGCCTTCCACATGAAGCTGTGGTCTCAGCCAGCAACCT
  GACCTTGGACTGTTTGACTGCAAAGCTTAGTGGCCTACAGGTGGCTGTAGAGACAGCCAATTTGATTTTG
  GATCTTTCATATGTTATTGAAGATAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRLEAKKPSLCKSEPLTTERVRTTLSVLKRIVTSCYGPSGRLKQLHNGFGGYVCTTSQSSALLSHLLVT
  HPILKILTASIQNHVSSFSDCGLFTAILCCNLIENVQRLGLTPTTVIRLNKHLLSLCISYLKSETCGCRI
  PVDFSSTQILLCLVRSILTSKPACMLTRKETEHVSALILRAFLLTIPENAEGHIILGKSLIVPLKGQRVI
  DSTVLPGILIEMSEVQLMRLLPIKKSTALKVALFCTTLSGDTSDTGEGTVVVSYGVSLENAVLDQLLNLG
  RQLISDHVDLVLCQKVIHPSLKQFLNMHRIIAIDRIGVTLMEPLTKMTGTQPIGSLGSICPNSYGSVKDV
  CTAKFGSKHFFHLIPNEATICSLLLCNRNDTAWDELKLTCQTALHVLQLTLKEPWALLGGGCTETHLAAY
  IRHKTHNDPESILKDDECTQTELQLIAEAFCSALESVVGSLEHDGGEILTDMKYGHLWSVQADSPCVANW
  PDLLSQCGCGLYNSQEELNWSFLRSTCRPFVPQSCLPHEAVVSASNLTLDCLTAKLSGLQVAVETANLIL
  DLSYVIEDKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170784
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170784.3
• RefSeq Size: 2960 bp
• RefSeq ORF: 1713 bp
• Locus ID: 8195
• UniProt ID: Q9NPJ1
• Cytogenetics: 20p12.2
• MW: 62.3 kDa
• Gene Summary: This gene encodes a protein which shares sequence similarity with other members of the type II chaperonin family. The encoded protein is a centrosome-shuttling protein and plays an important role in cytokinesis. This protein also interacts with other type II chaperonin members to form a complex known as the BBSome, which involves ciliary membrane biogenesis. This protein is encoded by a downstream open reading frame (dORF). Several upstream open reading frames (uORFs) have been identified, which repress the translation of the dORF, and two of which can encode small mitochondrial membrane proteins. Mutations in this gene have been observed in patients with Bardet-Biedl syndrome type 6, also known as McKusick-Kaufman syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]