Methionine Aminopeptidase 2 (METAP2) (NM_006838) Human Untagged Clone

CAT#: SC322467

METAP2 (untagged)-Human methionyl aminopeptidase 2 (METAP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Methionine Aminopeptidase 2
• Synonyms: MAP2; MNPEP; p67eIF2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322467 CTCTCGGGCAACATGGCGGGTGTGGAGGAGGTAGCGGCCTCCGGGAGCCACCTGAATGGC
  GACCTGGATCCAGACGACAGGGAAGAAGGAGCTGCCTCTACGGCTGAGGAAGCAGCCAAG
  AAAAAAAGACGAAAGAAGAAGAAGAGCAAAGGGCCTTCTGCAGCAGGGGAACAGGAACCT
  GATAAAGAATCAGGAGCCTCAGTGGATGAAGTAGCAAGACAGTTGGAAAGATCAGCATTG
  GAAGATAAAGAAAGAGATGAAGATGATGAAGATGGAGATGGCGATGGAGATGGAGCAACT
  GGAAAGAAGAAGAAAAAGAAGAAGAAGAAGAGAGGACCAAAAGTTCAAACAGACCCTCCC
  TCAGTTCCAATATGTGACCTGTATCCTAATGGTGTATTTCCCAAAGGACAAGAATGCGAA
  TACCCACCCACACAAGATGGGCGAACAGCTGCTTGGAGAACTACAAGTGAAGAAAAGAAA
  GCATTAGATCAGGCAAGTGAAGAGATTTGGAATGATTTTCGAGAAGCTGCAGAAGCACAT
  CGACAAGTTAGAAAATACGTAATGAGCTGGATCAAGCCTGGGATGACAATGATAGAAATC
  TGTGAAAAGTTGGAAGACTGTTCACGCAAGTTAATAAAAGAGAATGGATTAAATGCAGGC
  CTGGCATTTCCTACTGGATGTTCTCTCAATAATTGTGCTGCCCATTATACTCCCAATGCC
  GGTGACACAACAGTATTACAGTATGATGACATCTGTAAAATAGACTTTGGAACACATATA
  AGTGGTAGGATTATTGACTGTGCTTTTACTGTCACTTTTAATCCCAAATATGATACGTTA
  TTAAAAGCTGTAAAAGATGCTACTAACACTGGAATAAAGTGTGCTGGAATTGATGTTCGT
  CTGTGTGATGTTGGTGAGGCCATCCAAGAAGTTATGGAGTCCTATGAAGTTGAAATAGAT
  GGGAAGACATATCAAGTGAAACCAATCCGTAATCTAAATGGACATTCAATTGGGCAATAT
  AGAATACATGCTGGAAAAACAGTGCCGATTGTGAAAGGAGGGGAGGCAACAAGAATGGAG
  GAAGGAGAAGTATATGCAATTGAAACCTTTGGTAGTACAGGAAAAGGTGTTGTTCATGAT
  GATATGGAATGTTCACATTACATGAAAAATTTTGATGTTGGACATGTGCCAATAAGGCTT
  CCAAGAACAAAACACTTGTTAAATGTCATCAATGAAAACTTTGGAACCCTTGCCTTCTGC
  CGCAGATGGCTGGATCGCTTGGGAGAAAGTAAATACTTGATGGCTCTGAAGAATCTGTGT
  GACTTGGGCATTGTAGATCCATATCCACCATTATGTGACATTAAAGGATCATATACAGCG
  CAATTTGAACATACCATCCTGTTGCGTCCAACATGTAAAGAAGTTGTCAGCAGAGGAGAT
  GACTATTAAACTTAGTCCAAAGCCACCTCAACACCTTTATTTTCTGAGCTTTGTTGGAAA
  ACATGATACCAGAATTAATTTGCCACATGTTGTCTGTTTTAACAGTGGACCCATGTAATA
  CTTTTATCCATGTTTAAAAAAGAAGGAATTTGGACAAAGGCAAACCGTCTAATGTAATTA
  ACCAACGAAAAAGCTTTCCGGACTTTTAAATGCTAACTGTTTTTCCCCTTCCTGTCTAGG
  AAAATTCTATAAAGCTCAAATTAGTTAGGAATGACTTATACGTTTTGTTTTGAATACCTA
  AGAGATACTTTTTGGATATTTATATTGCCATATTCTTACTTGAATGCTTTGAATGACTAC
  ATCCAGTTCTGCACCTATACCCTCTGGTGTTGCTTTTTAACCTTCCTGGAATCCATTTTC
  TAAAAAATAAAGACATTTTCAGATCTGAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006838
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006838.3, NP_006829.1
• RefSeq Size: 3506 bp
• RefSeq ORF: 1437 bp
• Locus ID: 10988
• UniProt ID: P50579
• Cytogenetics: 12q22
• Domains: Peptidase_M24
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).