Methionine Aminopeptidase 2 (METAP2) (NM_006838) Human Untagged Clone

CAT#: SC115832

METAP2 (untagged)-Human methionyl aminopeptidase 2 (METAP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Methionine Aminopeptidase 2
• Synonyms: MAP2; MNPEP; p67eIF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115832 sequence for NM_006838 edited (data generated by NextGen Sequencing)
  ATGGCGGGTGTGGAGGAGGTAGCGGCCTCCGGGAGCCACCTGAATGGCGACCTGGATCCA
  GACGACAGGGAAGAAGGAGCTGCCTCTACGGCTGAGGAAGCAGCCAAGAAAAAAAGACGA
  AAGAAGAAGAAGAGCAAAGGGCCTTCTGCAGCAGGGGAACAGGAACCTGATAAAGAATCA
  GGAGCCTCAGTGGATGAAGTAGCAAGACAGTTGGAAAGATCAGCATTGGAAGATAAAGAA
  AGAGATGAAGATGATGAAGATGGAGATGGCGATGGAGATGGAGCAACTGGAAAGAAGAAG
  AAAAAGAAGAAGAAGAAGAGAGGACCAAAAGTTCAAACAGACCCTCCCTCAGTTCCAATA
  TGTGACCTGTATCCTAATGGTGTATTTCCCAAAGGACAAGAATGCGAATACCCACCCACA
  CAAGATGGGCGAACAGCTGCTTGGAGAACTACAAGTGAAGAAAAGAAAGCATTAGATCAG
  GCAAGTGAAGAGATTTGGAATGATTTTCGAGAAGCTGCAGAAGCACATCGACAAGTTAGA
  AAATACGTAATGAGCTGGATCAAGCCTGGGATGACAATGATAGAAATCTGTGAAAAGTTG
  GAAGACTGTTCACGCAAGTTAATAAAAGAGAATGGATTAAATGCAGGCCTGGCATTTCCT
  ACTGGATGTTCTCTCAATAATTGTGCTGCCCATTATACTCCCAATGCCGGTGACACAACA
  GTATTACAGTATGATGACATCTGTAAAATAGACTTTGGAACACATATAAGTGGTAGGATT
  ATTGACTGTGCTTTTACTGTCACTTTTAATCCCAAATATGATACGTTATTAAAAGCTGTA
  AAAGATGCTACTAACACTGGAATAAAGTGTGCTGGAATTGATGTTCGTCTGTGTGATGTT
  GGTGAGGCCATCCAAGAAGTTATGGAGTCCTATGAAGTTGAAATAGATGGGAAGACATAT
  CAAGTGAAACCAATCCGTAATCTAAATGGACATTCAATTGGGCAATATAGAATACATGCT
  GGAAAAACAGTGCCGATTGTGAAAGGAGGGGAGGCAACAAGAATGGAGGAAGGAGAAGTA
  TATGCAATTGAAACCTTTGGTAGTACAGGAAAAGGTGTTGTTCATGATGATATGGAATGT
  TCACATTACATGAAAAATTTTGATGTTGGACATGTGCCAATAAGGCTTCCAAGAACAAAA
  CACTTGTTAAATGTCATCAATGAAAACTTTGGAACCCTTGCCTTCTGCCGCAGATGGCTG
  GATCGCTTGGGAGAAAGTAAATACTTGATGGCTCTGAAGAATCTGTGTGACTTGGGCATT
  GTAGATCCATATCCACCATTATGTGACATTAAAGGATCATATACAGCGCAATTTGAACAT
  ACCATCCTGTTGCGTCCAACATGTAAAGAAGTTGTCAGCAGAGGAGATGACTATTAA
  
  Clone variation with respect to NM_006838.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006838 unedited
  NNNTTTGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAAG
  GATCGGGGCCCTCGCCGCTCTGTCTCATTCCCTCGCGCTCTCTCGGGCAACATGGCGGGT
  GTGGAGGAGGTAGCGGCCTCCGGGAGCCACCTGAATGGCGACCTGGATCCAGACGACAGG
  GAAGAAGGAGCTGCCTCTACGGCTGAGGAAGCAGCCAAGAAAAAAAGACGAAAGAAGAAG
  AAGAGCAAAGGGCCTTCTGCAGCAGGGGAACAGGAACCTGATAAAGAATCAGGAGCCTCA
  GTGGATGAAGTAGCAAGACAGTTGGAAAGATCAGCATTGGAAGATAAAGAAAGAGATGAA
  GATGATGAAGATGGAGATGGCGATGGAGATGGAGCAACTGGAAAGAAGAAGAAAAAGAAG
  AAGAAGAAGAGAGGACCAAAAGTTCAAACAGACCCTCCCTCAGTTCCAATATGTGACCTG
  TATCCTAATGGTGTATTTCCCAAAGGACAAGAATGCGAATACCCACCCACACAAGATGGG
  CGAACAGCTGCTTGGAGAACTACAAGTGAAGAAAAGAAAGCATTAGATCAGGCAAGTGAA
  GAGATTTGGAATGATTTTCGAGAAGCTGCAGAAGCACATCGACAAGTTAGAAAATACGTA
  ATGAGCTGGATCAAGCCTGNGATGACAATGATAGAAATCTGTGAAAAGTTGGAAGACTGT
  TCACGCAAGTTAATAAAAGAGAATGGATTAAATGCANGCCTGGCATTTCCTACTGGATGN
  TCTCTCAATAATTGTGCTGCCCATTATACTCCCNAATGCCGTGACACAACAGTATTACAG
  TATGATGACANTCTGTAAATAGACTTTGGAACCATATNAGTGGNTAGGGATATTGACTGT
  GNCTTTACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006838 unedited
  NNTTTTAGCTATGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGC
  TTTTGTGGCTTTAATACCCTTTTGGTTTTCTGAGCAAGCTGAATACATTCCCTGATAAAC
  ACCACCACTGGACAAAGGATCTAAACATTGAGATTTTTACTAAATCAAGGTTTTGAGTTG
  GCACAATGTTCCTACTAAGTCAAGTTTCAATTAGCACATCTTTCCAATGTCAGGAGAAAA
  AAAGACAAGGACTACTATGTCCTACAAAGAATGGTGAAATATTCCTAAAGCTGTATATTT
  ATGAAGAAAAGGTGATTGTCCCTTTTTATGCATGTGTAGATCTTGTTTTAGCCACTATGC
  CAGAAAAGTCTCAGTGTAGAGGTCTACATTTCAGATAAATGTCTCTGCATTTGCCTACAT
  TAAGTTTAGCTATTTATCCTGTCCAGAATTATAACCACAGACATTGAGATGTAGCTCTCA
  GATCTGAAAATGTCTTTATTTTTTAGAAAATGGATTCCAGGAAGGTTAAAAAGCAACACC
  AGAGGGTATAGGTGCAGAACTGGATGTAGTCATTCAAAGCATTCAAGTAAGAATATGGCA
  ATATAAATATCCAAAAAGTATCTCTTAGGTATTCAAAACAAAACGTATAAGTCATTCCTA
  ACTAATTTGAGCTTTATAGCATTTTCCTAGACAGGAAGGGGAAAAACAGTTAGCATTTAA
  AAGTCCGGAAAGCTTTTTCGTTGGTTAATTACATTAGACGGTTTGCCTTTGTCCAAATTC
  CTTCTTTTTTAAACATGGNATANAGTATTACATGGGTCCACTGNTAANAACAGACACATG
  TGGCANATTAATTCTGGTATCATGTTTTNCCACAAAGCTCAGAAATAAAGTGNTGAGGTG
  GCTTGGACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006838
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006838.2, NP_006829.1
• RefSeq Size: 1908 bp
• RefSeq ORF: 1437 bp
• Locus ID: 10988
• UniProt ID: P50579
• Cytogenetics: 12q22
• Domains: Peptidase_M24
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).