LRRC25 (NM_145256) Human Untagged Clone

CAT#: SC321936

LRRC25 (untagged)-Human leucine rich repeat containing 25 (LRRC25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRRC25
• Synonyms: MAPA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145256.2 GTAGGTTGCTTGAATGGGGGGCACCCTGGCATGGACGCTGCTGTTGCCGCTGCTGCTGCG
  GGAGTCAGACAGCCTAGAACCGTCGTGCACCGTGTCCTCCGCGGATGTGGACTGGAACGC
  GGAGTTCAGTGCCACGTGCCTGAATTTCAGTGGCCTCAGCCTGAGCCTGCCTCACAACCA
  GTCTCTGCGGGCCAGCAACGTGATTCTCCTTGACCTGTCTGGGAACGGCCTGCGAGAGCT
  TCCAGTGACCTTCTTTGCCCACCTGCAGAAGCTTGAGGTCCTGAACGTGCTACGCAACCC
  CTTGTCTCGTGTGGATGGGGCGCTGGCCGCCCGCTGTGACCTTGACCTGCAGGCCGACTG
  CAACTGTGCCCTGGAGTCCTGGCACGACATCCGCCGAGACAACTGCTCTGGCCAGAAGCC
  TCTGCTCTGCTGGGACACAACCAGCTCCCAGCACAACCTCTCTGCCTTCCTGGAGGTCAG
  CTGCGCCCCTGGCCTGGCCTCTGCAACTATCGGGGCAGTGGTGGTCAGCGGGTGCCTGCT
  TCTTGGACTTGCCATCGCTGGCCCTGTGCTGGCCTGGAGACTCTGGCGATGCCGAGTGGC
  CAGAAGCCGGGAGCTGAACAAACCCTGGGCTGCTCAGGATGGGCCCAAGCCCGGTTTAGG
  CTTGCAGCCACGGTACGGCAGCCGGAGCGCCCCCAAGCCCCAAGTGGCCGTGCCATCCTG
  CCCCTCCACTCCCGACTATGAGAACATGTTTGTGGGCCAGCCAGCAGCCGAGCACCAGTG
  GGATGAACAAGGGGCTCACCCTTCAGAGGACAATGACTTTTACATCAACTACAAGGACAT
  CGACCTGGCTTCCCAGCCTGTCTACTGTAACCTGCAGTCACTGGGCCAGGCCTCAATGGA
  TGAAGAGGAGTACGTGATCCCCGGGCACTGAGCCTAAGATGTCCTAACCTCCACCCAGAA
  CCCCTTCAGTCCCTGCTGGGTGACTCAGGGCGTCCTAACTCCTCCATGGCCTCAGTTTCC
  CCATCTGAAGAATGGGGACAGGAAAGGATTGTCCTTGAGGCCCCAGGAAGCTCTGCCGCC
  CCCTCCCTGTCCCTCATGCCGCTCCTCAGCTCCCTCAGCTCCTAGAGGGGGAAGAGGAGA
  GACCCCCAACAAGGGGACAGGAGGGTCACTGTGCCAATCCTGTCATCACCCTCCTGTGGA
  TGTACAGGCAGTGCTCAATAAATGCTTCGAGGCTGATGAGGCTGCTGGCTCAGGGTGCGT
  GGGTTCCTCAAGGTGGGGATTTCTGAGTTCTAAGACCAAGTCTCCATCTGAGACTCCCAA
  ATTGCTCCCCACCTCCCATCCCTGTTTTTTTTTGTTGTTGTTGTTTGTTTGTTTGTTTTT
  GAAACTGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAATGCTGCGATCTCAGCTCACTG
  CAACCTCCGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGAT
  TACAGCACCCGCCACCATGCCGAGCTAATTTTTGTATTTATAATAGAGATGGGGTTTCGC
  CATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGCGATCTGCCCACCTCGGCCTCC
  TGAAGTGCTGGGATTACAGGCGTGGCCACCGCACCCAGGCACATTCCTCCCTTCTGCCCC
  TCTCAGGGCCCCTTCCCAGGTCCCTGATCTCCAGGCTTGGCCTCCAGAGCAGCCCACACC
  ACCCCCAAAATAAAAAAATGTATATATTCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_145256
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145256.2, NP_660299.2
• RefSeq Size: 2430 bp
• RefSeq ORF: 918 bp
• Locus ID: 126364
• UniProt ID: Q8N386
• Cytogenetics: 19p13.11
• Domains: LRR
• Protein Families: Transmembrane
• Gene Summary: Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation. Interacts specifically with ISG15-associated DDX58 to promote interaction between DDX58 and the autophagic cargo receptor p62/SQSTM1 to mediate DDX58 degradation via selective autophagy (PubMed:29288164). Plays also a role in the inhibition of NF-kappa-B signaling pathway and inflammatory response by promoting the degradation of p65/RELA.[UniProtKB/Swiss-Prot Function]