LRRC25 (NM_145256) Human Untagged Clone

CAT#: SC100131

LRRC25 (untagged)-Human leucine rich repeat containing 25 (LRRC25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRRC25
• Synonyms: MAPA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100131 sequence for NM_145256 edited (data generated by NextGen Sequencing)
  ATGGGGGGCACCCTGGCATGGACGCTGCTGTTGCCGCTGCTGCTGCGGGAGTCAGACAGC
  CTAGAACCGTCGTGCACCGTGTCCTCCGCGGATGTGGACTGGAACGCGGAGTTCAGTGCC
  ACGTGCCTGAATTTCAGTGGCCTCAGCCTGAGCCTGCCTCACAACCAGTCTCTGCGGGCC
  AGCAACGTGATTCTCCTTGACCTGTCTGGGAACGGCCTGCGAGAGCTTCCAGTGACCTTC
  TTTGCCCACCTGCAGAAGCTTGAGGTCCTGAACGTGCTACGCAACCCCTTGTCTCGTGTG
  GATGGGGCGCTGGCCGCCCGCTGTGACCTTGACCTGCAGGCCGACTGCAACTGTGCCCTG
  GAGTCCTGGCACGACATCCGCCGAGACAACTGCTCTGGCCAGAAGCCTCTGCTCTGCTGG
  GACACAACCAGCTCCCAGCACAACCTCTCTGCCTTCCTGGAGGTCAGCTGCGCCCCTGGC
  CTGGCCTCTGCAACTATCGGGGCAGTGGTGGTCAGCGGGTGCCTGCTTCTTGGACTTGCC
  ATCGCTGGCCCTGTGCTGGCCTGGAGACTCTGGCGATGCCGAGTGGCCAGAAGCCGGGAG
  CTGAACAAACCCTGGGCTGCTCAGGATGGGCCCAAGCCCGGTTTAGGCTTGCAGCCACGG
  TACGGCAGCCGGAGCGCCCCCAAGCCCCAAGTGGCCGTGCCATCCTGCCCCTCCACTCCC
  GACTATGAGAACATGTTTGTGGGCCAGCCAGCAGCCGAGCACCAGTGGGATGAACAAGGG
  GCTCACCCTTCAGAGGACAATGACTTTTACATCAACTACAAGGACATCGACCTGGCTTCC
  CAGCCTGTCTACTGTAACCTGCAGTCACTGGGCCAGGCCTCAATGGATGAAGAGGAGTAC
  GTGATCCCCGGGCACTGA
  
  Clone variation with respect to NM_145256.2
  880 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145256 unedited
  GGAATTTGTAAACGACTTACTATAGGGNNCGGCCGCGNNAATTCGGCACGAGGGAACGCC
  AGCGACCCCAGCAGCGCTGCGGACGGTGCTGGCCGTGGCCGCTGCGGCCCCCGTGTCCAG
  GTGGGCCAGGACGCAGCCTCTGGGCGCCGTCGCTTTTCCAGCATCGCAGAGGCAAAAGCG
  TGGCAGTGGGACCCAAAAGGTTGCTTGAATGGGGGGCACCCTGGCATGGACGCTGCTGTT
  GCCGCTGCTGCTGCGGGAGTCAGACAGCCTAGAACCGTCGTGCACCGTGTCCTCCGCGGA
  TGTGGACTGGAACGCGGAGTTCAGTGCCACGTGCCTGAATTTCAGTGGCCTCAGCCTGAG
  CCTGCCTCACAACCAGTCTCTGCGGGCCAGCAACGTGATTCTCCTTGACCTGTCTGGGAA
  CGGCCTGCGAGAGCTTCCAGTGACCTTCTTTGCCCACCTGCAGAAGCTTGAGGTCCTGAA
  CGTGCTACGCAACCCCTTGTCTCGTGTGGATGGGGCGCTGGCCGCCCGCTGTGACCTTGA
  CCTGCAGGCCGACTGCAACTGTGCCCTGGAGTCCTGGCACGACATCCGCCGAGACAACTG
  CTCTGGCCAGAAGCCTCTGCTCTGCTGGGACACAACCAGCTCCCAGCACAACCTCTCTGC
  CTTCCTGGAGGTCAGCTGCGCCCCTGGCCTGGCCTCTGCAACTATCGGGGCAGTGGTGGT
  CAGCGGGTGCCTGCTTCTTGGACTTGCCATCGCTGGCCCTGTGCTGGCCTGGAGACTCTG
  GCGATGCCGAGTGGCCAGAAGCCGGGAGCTGAACANACCCTGGGCTGCTCANGATGGGCC
  CAAAGCCCGGTTTAAGCTTGCAGCCACGGTACGGCAGCCGGAGCGCCCNCAGCCCCAGTG
  GCCGTGCCATCTGCCCTCCACTCCCGATATGAGAACATGTTGTGGCCANCAGCAGCGAGC
  ACAGTGGATGAACAGGGCTCACCTTCAGAGACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145256
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145256.1, NP_660299.1
• RefSeq Size: 1963 bp
• RefSeq ORF: 303 bp
• Locus ID: 126364
• UniProt ID: Q8N386
• Cytogenetics: 19p13.11
• Domains: LRR
• Protein Families: Transmembrane
• Gene Summary: Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation. Interacts specifically with ISG15-associated DDX58 to promote interaction between DDX58 and the autophagic cargo receptor p62/SQSTM1 to mediate DDX58 degradation via selective autophagy (PubMed:29288164). Plays also a role in the inhibition of NF-kappa-B signaling pathway and inflammatory response by promoting the degradation of p65/RELA.[UniProtKB/Swiss-Prot Function]