LRRC25 (NM_145256) Human Tagged ORF Clone

CAT#: RC209911

LRRC25 (Myc-DDK-tagged)-Human leucine rich repeat containing 25 (LRRC25)

Product Images

Circular map for RC209911

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LRRC25 (Cat# RC209911, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC25(Cat# TA504883). Positive lysates LY407925 (100ug) and LC407925 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY407925) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209911 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC25
• Synonyms: MAPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209911 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGCACCCTGGCATGGACGCTGCTGTTGCCGCTGCTGCTGCGGGAGTCAGACAGCCTAGAACCGT
  CGTGCACCGTGTCCTCCGCGGATGTGGACTGGAACGCGGAGTTCAGTGCCACGTGCCTGAATTTCAGTGG
  CCTCAGCCTGAGCCTGCCTCACAACCAGTCTCTGCGGGCCAGCAACGTGATTCTCCTTGACCTGTCTGGG
  AACGGCCTGCGAGAGCTTCCAGTGACCTTCTTTGCCCACCTGCAGAAGCTTGAGGTCCTGAACGTGCTAC
  GCAACCCCTTGTCTCGTGTGGATGGGGCGCTGGCCGCCCGCTGTGACCTTGACCTGCAGGCCGACTGCAA
  CTGTGCCCTGGAGTCCTGGCACGACATCCGCCGAGACAACTGCTCTGGCCAGAAGCCTCTGCTCTGCTGG
  GACACAACCAGCTCCCAGCACAACCTCTCTGCCTTCCTGGAGGTCAGCTGCGCCCCTGGCCTGGCCTCTG
  CAACTATCGGGGCAGTGGTGGTCAGCGGGTGCCTGCTTCTTGGACTTGCCATCGCTGGCCCTGTGCTGGC
  CTGGAGACTCTGGCGATGCCGAGTGGCCAGAAGCCGGGAGCTGAACAAACCCTGGGCTGCTCAGGATGGG
  CCCAAGCCCGGTTTAGGCTTGCAGCCACGGTACGGCAGCCGGAGCGCCCCCAAGCCCCAAGTGGCCGTGC
  CATCCTGCCCCTCCACTCCCGACTATGAGAACATGTTTGTGGGCCAGCCAGCAGCCGAGCACCAGTGGGA
  TGAACAAGGGGCTCACCCTTCAGAGGACAATGACTTTTACATCAACTACAAGGACATCGACCTGGCTTCC
  CAGCCTGTCTACTGTAACCTGCAGTCACTGGGCCAGGCCTCAATGGATGAAGAGGAGTACGTGATCCCCG
  GGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209911 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGGTLAWTLLLPLLLRESDSLEPSCTVSSADVDWNAEFSATCLNFSGLSLSLPHNQSLRASNVILLDLSG
  NGLRELPVTFFAHLQKLEVLNVLRNPLSRVDGALAARCDLDLQADCNCALESWHDIRRDNCSGQKPLLCW
  DTTSSQHNLSAFLEVSCAPGLASATIGAVVVSGCLLLGLAIAGPVLAWRLWRCRVARSRELNKPWAAQDG
  PKPGLGLQPRYGSRSAPKPQVAVPSCPSTPDYENMFVGQPAAEHQWDEQGAHPSEDNDFYINYKDIDLAS
  QPVYCNLQSLGQASMDEEEYVIPGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145256
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145256.2, NP_660299.2
• RefSeq Size: 2430 bp
• RefSeq ORF: 918 bp
• Locus ID: 126364
• UniProt ID: Q8N386
• Cytogenetics: 19p13.11
• Domains: LRR
• Protein Families: Transmembrane
• MW: 33.2 kDa
• Gene Summary: Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation. Interacts specifically with ISG15-associated DDX58 to promote interaction between DDX58 and the autophagic cargo receptor p62/SQSTM1 to mediate DDX58 degradation via selective autophagy (PubMed:29288164). Plays also a role in the inhibition of NF-kappa-B signaling pathway and inflammatory response by promoting the degradation of p65/RELA.[UniProtKB/Swiss-Prot Function]