KDM4A (NM_014663) Human Untagged Clone

CAT#: SC321310

KDM4A (untagged)-Human lysine (K)-specific demethylase 4A (KDM4A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDM4A
• Synonyms: JHDM3A; JMJD2; JMJD2A; TDRD14A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014663.2 GGAGTTTCGGCCTTCGCCTGCTGGAAAAGCAGTAGGATCGGCCAGTGGCGACAGCAGGAG
  CTGAGCCTAAGCCCTGGCGGGGCTTTGGGCTGTAGATTCCTGTCTGACTAAAGGGACCTC
  AAAAAGGAGGGAAAATGGCTTCTGAGTCTGAAACTCTGAATCCCAGTGCTAGGATAATGA
  CCTTTTATCCAACTATGGAAGAGTTCCGAAACTTCAGTAGATACATTGCCTACATTGAAT
  CCCAAGGAGCTCATCGGGCAGGGCTAGCCAAGGTTGTTCCTCCAAAAGAGTGGAAGCCAC
  GAGCATCCTATGATGACATTGATGATTTGGTCATTCCTGCCCCCATTCAACAGCTGGTGA
  CGGGGCAGTCTGGCCTCTTTACTCAGTACAACATACAGAAGAAAGCCATGACTGTTCGAG
  AGTTCCGCAAGATAGCCAATAGCGATAAGTACTGTACCCCACGCTATAGTGAGTTTGAAG
  AGCTCGAGCGGAAATACTGGAAAAATCTTACATTCAATCCTCCAATCTATGGTGCAGATG
  TGAATGGTACCCTCTATGAAAAGCATGTTGATGAGTGGAATATTGGCCGGCTGAGAACAA
  TCCTGGACTTGGTGGAAAAGGAGAGTGGGATCACCATTGAGGGTGTGAACACCCCATACC
  TGTACTTTGGCATGTGGAAGACATCCTTTGCTTGGCACACTGAAGACATGGACCTCTACA
  GCATCAACTACCTGCACTTTGGAGAACCAAAGTCCTGGTACTCTGTTCCACCTGAGCATG
  GAAAGCGGTTGGAACGCCTCGCCAAAGGCTTTTTCCCAGGAAGTGCTCAAAGCTGTGAGG
  CATTTCTCCGCCACAAGATGACCCTGATTTCCCCGTTAATGCTGAAGAAATATGGAATTC
  CCTTTGACAAGGTGACTCAAGAGGCTGGAGAGTTTATGATCACTTTCCCTTATGGTTACC
  ATGCCGGCTTTAACCATGGTTTTAACTGTGCGGAGTCTACCAATTTTGCTACCCGTCGGT
  GGATTGAGTACGGCAAGCAAGCTGTGCTGTGCTCCTGTAGAAAGGACATGGTGAAGATCT
  CCATGGATGTGTTTGTGAGAAAGTTCCAGCCAGAAAGGTACAAACTTTGGAAAGCTGGGA
  AGGACAACACAGTTATTGACCATACTCTGCCCACGCCAGAAGCAGCTGAGTTTCTTAAGG
  AGAGTGAACTGCCTCCAAGAGCTGGCAACGAGGAGGAGTGCCCAGAGGAGGACATGGAAG
  GGGTGGAGGATGGAGAGGAAGGAGACCTGAAGACAAGCCTGGCCAAGCACCGAATAGGGA
  CAAAGAGGCACCGAGTTTGTCTTGAAATACCACAGGAGGTGAGTCAGAGTGAGCTCTTCC
  CCAAGGAGGATCTGAGTTCTGAGCAGTATGAGATGACGGAGTGCCCGGCAGCCCTCGCCC
  CTGTGAGGCCCACCCATAGCTCTGTGCGGCAAGTTGAGGATGGTCTTACCTTCCCAGATT
  ATTCTGACTCCACTGAAGTCAAATTTGAAGAGCTTAAAAATGTCAAACTAGAAGAGGAGG
  ATGAGGAGGAAGAACAAGAAGCAGCTGCCTTGGATCTTTCTGTGAATCCTGCGTCTGTAG
  GGGGACGCCTTGTCTTCTCAGGCTCCAAAAAGAAATCATCTTCTAGCCTGGGCTCTGGCT
  CTTCACGGGATTCTATCTCTTCTGATTCAGAAACTAGTGAGCCTCTCTCCTGCCGAGCCC
  AAGGGCAAACGGGAGTTCTCACTGTGCACAGTTATGCCAAAGGGGATGGCAGGGTCACTG
  TGGGAGAGCCATGCACGAGGAAGAAAGGAAGCGCCGCTAGAAGTTTCAGTGAGCGGGAGC
  TGGCAGAGGTTGCAGATGAATACATGTTTTCCCTAGAAGAGAATAAGAAGTCCAAGGGAC
  GCCGTCAGCCTTTAAGCAAGCTCCCCCGCCATCACCCACTTGTGCTGCAGGAGTGTGTCA
  GTGATGATGAGACATCTGAACAGCTGACCCCTGAGGAAGAGGCTGAGGAGACAGAGGCCT
  GGGCCAAGCCTCTGAGCCAACTGTGGCAGAACCGACCTCCAAACTTTGAGGCTGAGAAGG
  AATTCAATGAGACCATGGCCCAACAGGCCCCTCACTGCGCTGTCTGTATGATCTTCCAGA
  CTTATCATCAGGTTGAATTTGGAGGCTTTAATCAGAACTGTGGAAATGCTTCAGATTTAG
  CCCCCCAGAAGCAGAGGACCAAGCCATTGATTCCAGAAATGTGCTTCACTTCGACTGGCT
  GCAGCACGGACATCAACCTTTCTACTCCTTATCTTGAGGAGGATGGCACCAGCATACTCG
  TTTCCTGCAAGAAGTGCAGCGTCCGGGTCCATGCCAGTTGCTATGGGGTCCCCCCTGCAA
  AGGCTTCTGAAGACTGGATGTGTTCTCGGTGTTCAGCCAATGCCCTAGAGGAGGACTGCT
  GTTTATGCTCATTACGAGGAGGGGCCCTGCAGAGAGCAAATGATGACAGGTGGGTCCACG
  TTTCATGTGCTGTGGCAATTCTGGAAGCAAGGTTTGTCAACATTGCAGAAAGAAGTCCGG
  TGGATGTGAGCAAAATCCCCCTGCCCCGCTTCAAACTGAAATGTATCTTCTGTAAGAAGC
  GGAGGAAAAGAACTGCTGGCTGCTGTGTGCAGTGTTCTCACGGCCGCTGCCCAACTGCCT
  TCCATGTGAGCTGCGCCCAGGCTGCCGGTGTGATGATGCAGCCTGACGACTGGCCTTTTG
  TGGTCTTCATTACCTGCTTTCGGCACAAGATTCCTAATTTGGAGCGTGCCAAGGGGGCCT
  TGCAAAGCATCACTGCAGGCCAGAAAGTCATTAGCAAGCATAAGAACGGGCGCTTCTACC
  AGTGTGAAGTGGTCAGGCTCACCACCGAGACCTTCTATGAAGTCAACTTTGATGATGGCT
  CCTTCAGCGACAATCTTTATCCTGAGGACATAGTGAGCCAGGACTGTCTCCAGTTTGGTC
  CTCCTGCTGAAGGGGAAGTGGTCCAAGTGAGATGGACAGACGGCCAAGTCTATGGAGCCA
  AGTTTGTGGCCTCCCACCCTATCCAAATGTACCAGGTGGAGTTTGAGGATGGCTCACAAC
  TTGTGGTTAAGAGAGATGATGTATACACACTGGATGAAGAGCTTCCCAAGAGAGTCAAAT
  CTAGACTGTCAGTAGCCTCAGACATGCGCTTCAATGAGATTTTCACAGAGAAAGAGGTTA
  AGCAAGAAAAGAAACGGCAACGAGTTATCAACTCAAGATACCGGGAAGATTATATTGAGC
  CTGCACTATACCGGGCCATCATGGAGTAGGTGCTTCCAGGGTCCAAGGGATTCTCAGCCA
  TCCAGGCAAGAGCACTCTGGGTTCCACAGCACAGCAGACATGGAACGCTGAAGTCTCTGA
  AAGTGAAGTTGTAAAAAGAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014663
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014663.2, NP_055478.2
• RefSeq Size: 4526 bp
• RefSeq ORF: 3195 bp
• Locus ID: 9682
• UniProt ID: O75164
• Cytogenetics: 1p34.2-p34.1
• Domains: PHD, TUDOR, JmjC, JmjN
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]