KDM4A (NM_014663) Human Tagged ORF Clone

CAT#: RG200574

KDM4A (tGFP-tagged) - Human lysine (K)-specific demethylase 4A (KDM4A)

Product Images

Circular map for RG200574

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KDM4A
• Synonyms: JHDM3A; JMJD2; JMJD2A; TDRD14A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200574 representing NM_014663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGAGTCTGAAACTCTGAATCCCAGTGCTAGGATAATGACCTTTTATCCAACTATGGAAGAGT
  TCCGAAACTTCAGTAGATACATTGCCTACATTGAATCCCAAGGAGCTCATCGGGCAGGGCTAGCCAAGGT
  TGTTCCTCCAAAAGAGTGGAAGCCACGAGCATCCTATGATGACATTGATGATTTGGTCATTCCTGCCCCC
  ATTCAACAGCTGGTGACGGGGCAGTCTGGCCTCTTTACTCAGTACAACATACAGAAGAAAGCCATGACTG
  TTCGAGAGTTCCGCAAGATAGCCAATAGCGATAAGTACTGTACCCCACGCTATAGTGAGTTTGAAGAGCT
  CGAGCGGAAATACTGGAAAAATCTTACATTCAATCCTCCAATCTATGGTGCAGATGTGAATGGTACCCTC
  TATGAAAAGCATGTTGATGAGTGGAATATTGGCCGGCTGAGAACAATCCTGGACTTGGTGGAAAAGGAGA
  GTGGGATCACCATTGAGGGTGTGAACACCCCATACCTGTACTTTGGCATGTGGAAGACATCCTTTGCTTG
  GCACACTGAAGACATGGACCTCTACAGCATCAACTACCTGCACTTTGGAGAACCAAAGTCCTGGTACTCT
  GTTCCACCTGAGCATGGAAAGCGGTTGGAACGCCTCGCCAAAGGCTTTTTCCCAGGAAGTGCTCAAAGCT
  GTGAGGCATTTCTCCGCCACAAGATGACCCTGATTTCCCCGTTAATGCTGAAGAAATATGGAATTCCCTT
  TGACAAGGTGACTCAAGAGGCTGGAGAGTTTATGATCACTTTCCCTTATGGTTACCATGCCGGCTTTAAC
  CATGGTTTTAACTGTGCGGAGTCTACCAATTTTGCTACCCGTCGGTGGATTGAGTACGGCAAGCAAGCTG
  TGCTGTGCTCCTGTAGAAAGGACATGGTGAAGATCTCCATGGATGTGTTTGTGAGAAAGTTCCAGCCAGA
  AAGGTACAAACTTTGGAAAGCTGGGAAGGACAACACAGTTATTGACCATACTCTGCCCACGCCAGAAGCA
  GCTGAGTTTCTTAAGGAGAGTGAACTGCCTCCAAGAGCTGGCAACGAGGAGGAGTGCCCAGAGGAGGACA
  TGGAAGGGGTGGAGGATGGAGAGGAAGGAGACCTGAAGACAAGCCTGGCCAAGCACCGAATAGGGACAAA
  GAGGCACCGAGTTTGTCTTGAAATACCACAGGAGGTGAGTCAGAGTGAGCTCTTCCCCAAGGAGGATCTG
  AGTTCTGAGCAGTATGAGATGACGGAGTGCCCGGCAGCCCTCGCCCCTGTGAGGCCCACCCATAGCTCTG
  TGCGGCAAGTTGAGGATGGTCTTACCTTCCCAGATTATTCTGACTCCACTGAAGTCAAATTTGAAGAGCT
  TAAAAATGTCAAACTAGAAGAGGAGGATGAGGAGGAAGAACAAGAAGCAGCTGCCTTGGATCTTTCTGTG
  AATCCTGCGTCTGTAGGGGGACGCCTTGTCTTCTCAGGCTCCAAAAAGAAATCATCTTCTAGCCTGGGCT
  CTGGCTCTTCACGGGATTCTATCTCTTCTGATTCAGAAACTAGTGAGCCTCTCTCCTGCCGAGCCCAAGG
  GCAAACGGGAGTTCTCACTGTGCACAGTTATGCCAAAGGGGATGGCAGGGTCACTGTGGGAGAGCCATGC
  ACGAGGAAGAAAGGAAGCGCCGCTAGAAGTTTCAGTGAGCGGGAGCTGGCAGAGGTTGCAGATGAATACA
  TGTTTTCCCTAGAAGAGAATAAGAAGTCCAAGGGACGCCGTCAGCCTTTAAGCAAGCTCCCCCGCCATCA
  CCCACTTGTGCTGCAGGAGTGTGTCAGTGATGATGAGACATCTGAACAGCTGACCCCTGAGGAAGAGGCT
  GAGGAGACAGAGGCCTGGGCCAAGCCTCTGAGCCAACTGTGGCAGAACCGACCTCCAAACTTTGAGGCTG
  AGAAGGAATTCAATGAGACCATGGCCCAACAGGCCCCTCACTGCGCTGTCTGTATGATCTTCCAGACTTA
  TCATCAGGTTGAATTTGGAGGCTTTAATCAGAACTGTGGAAATGCTTCAGATTTAGCCCCCCAGAAGCAG
  AGGACCAAGCCATTGATTCCAGAAATGTGCTTCACTTCGACTGGCTGCAGCACGGACATCAACCTTTCTA
  CTCCTTATCTTGAGGAGGATGGCACCAGCATACTCGTTTCCTGCAAGAAGTGCAGCGTCCGGGTCCATGC
  CAGTTGCTATGGGGTCCCCCCTGCAAAGGCTTCTGAAGACTGGATGTGTTCTCGGTGTTCAGCCAATGCC
  CTAGAGGAGGACTGCTGTTTATGCTCATTACGAGGAGGGGCCCTGCAGAGAGCAAATGATGACAGGTGGG
  TCCACGTTTCATGTGCTGTGGCAATTCTGGAAGCAAGGTTTGTCAACATTGCAGAAAGAAGTCCGGTGGA
  TGTGAGCAAAATCCCCCTGCCCCGCTTCAAACTGAAATGTATCTTCTGTAAGAAGCGGAGGAAAAGAACT
  GCTGGCTGCTGTGTGCAGTGTTCTCACGGCCGCTGCCCAACTGCCTTCCATGTGAGCTGCGCCCAGGCTG
  CCGGTGTGATGATGCAGCCTGACGACTGGCCTTTTGTGGTCTTCATTACCTGCTTTCGGCACAAGATTCC
  TAATTTGGAGCGTGCCAAGGGGGCCTTGCAAAGCATCACTGCAGGCCAGAAAGTCATTAGCAAGCATAAG
  AACGGGCGCTTCTACCAGTGTGAAGTGGTCAGGCTCACCACCGAGACCTTCTATGAAGTCAACTTTGATG
  ATGGCTCCTTCAGCGACAATCTTTATCCTGAGGACATAGTGAGCCAGGACTGTCTCCAGTTTGGTCCTCC
  TGCTGAAGGGGAAGTGGTCCAAGTGAGATGGACAGACGGCCAAGTCTATGGAGCCAAGTTTGTGGCCTCC
  CACCCTATCCAAATGTACCAGGTGGAGTTTGAGGATGGCTCACAACTTGTGGTTAAGAGAGATGATGTAT
  ACACACTGGATGAAGAGCTTCCCAAGAGAGTCAAATCTAGACTGTCAGTAGCCTCAGACATGCGCTTCAA
  TGAGATTTTCACAGAGAAAGAGGTTAAGCAAGAAAAGAAACGGCAACGAGTTATCAACTCAAGATACCGG
  GAAGATTATATTGAGCCTGCACTATACCGGGCCATCATGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200574 representing NM_014663
  Red=Cloning site Green=Tags(s)
  
  MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRASYDDIDDLVIPAP
  IQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTL
  YEKHVDEWNIGRLRTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYS
  VPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFN
  HGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEA
  AEFLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKTSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDL
  SSEQYEMTECPAALAPVRPTHSSVRQVEDGLTFPDYSDSTEVKFEELKNVKLEEEDEEEEQEAAALDLSV
  NPASVGGRLVFSGSKKKSSSSLGSGSSRDSISSDSETSEPLSCRAQGQTGVLTVHSYAKGDGRVTVGEPC
  TRKKGSAARSFSERELAEVADEYMFSLEENKKSKGRRQPLSKLPRHHPLVLQECVSDDETSEQLTPEEEA
  EETEAWAKPLSQLWQNRPPNFEAEKEFNETMAQQAPHCAVCMIFQTYHQVEFGGFNQNCGNASDLAPQKQ
  RTKPLIPEMCFTSTGCSTDINLSTPYLEEDGTSILVSCKKCSVRVHASCYGVPPAKASEDWMCSRCSANA
  LEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCIFCKKRRKRT
  AGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALQSITAGQKVISKHK
  NGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVSQDCLQFGPPAEGEVVQVRWTDGQVYGAKFVAS
  HPIQMYQVEFEDGSQLVVKRDDVYTLDEELPKRVKSRLSVASDMRFNEIFTEKEVKQEKKRQRVINSRYR
  EDYIEPALYRAIME
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014663
• ORF Size: 3192 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014663.1, NP_055478.1
• RefSeq Size: 4526 bp
• RefSeq ORF: 3195 bp
• Locus ID: 9682
• UniProt ID: O75164
• Cytogenetics: 1p34.2-p34.1
• Domains: PHD, TUDOR, JmjC, JmjN
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]