ADH4 (NM_000670) Human Untagged Clone

CAT#: SC320964

ADH4 (untagged)-Human alcohol dehydrogenase 4 (class II), pi polypeptide (ADH4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADH4
• Synonyms: ADH-2; HEL-S-4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000670.3 ACGGAACTCCCTGGGTAGGAGTTTGAAGCTTTCTTAACTCAGAAAGAAACTTCCAACACA
  GTTTCCCAAAGAAAAATGGGCACCAAGGGCAAAGTTATTAAATGCAAAGCAGCCATCGCC
  TGGGAAGCAGGCAAGCCCCTTTGCATTGAAGAGGTTGAAGTAGCTCCCCCCAAGGCTCAT
  GAAGTTCGCATTCAGATCATTGCTACCTCCCTGTGCCATACTGATGCCACTGTTATCGAT
  TCTAAATTTGAGGGCCTAGCTTTCCCAGTGATCGTTGGCCATGAGGCTGCAGGTATTGTG
  GAAAGTATTGGGCCAGGAGTGACCAACGTCAAACCAGGTGACAAAGTAATTCCACTTTAT
  GCACCTCTATGTAGAAAATGCAAGTTTTGTCTGAGTCCACTCACAAATTTGTGTGGGAAA
  ATCAGTAATCTCAAAAGTCCTGCTAGTGATCAACAACTAATGGAAGACAAAACCAGCAGG
  TTTACCTGCAAAGGAAAACCAGTTTACCATTTCTTTGGAACCAGTACATTCTCTCAGTAC
  ACTGTGGTGTCAGATATCAATCTTGCCAAAATAGATGATGATGCAAATTTAGAGAGAGTT
  TGTCTGCTTGGATGTGGGTTTTCAACTGGCTATGGGGCTGCAATCAACAATGCCAAGGTC
  ACCCCTGGTTCGACTTGTGCTGTCTTTGGCCTAGGAGGTGTGGGTCTTTCTGCTGTAATG
  GGTTGTAAAGCAGCAGGAGCTTCCAGAATCATAGGTATTGACATCAACAGTGAGAAGTTT
  GTGAAGGCTAAAGCCCTGGGAGCCACTGACTGCCTCAATCCTAGAGACTTACATAAACCT
  ATCCAGGAAGTTATCATTGAATTGACCAAGGGAGGTGTGGATTTTGCCCTTGACTGTGCA
  GGTGGATCTGAAACCATGAAAGCAGCCCTGGACTGTACAACCGCAGGCTGGGGATCATGT
  ACTTTCATTGGAGTAGCTGCTGGTAGCAAAGGATTGACTGTTTTTCCAGAGGAGCTAATA
  ATCGGCCGTACTATAAATGGAACATTCTTTGGTGGTTGGAAAAGTGTAGATTCTATCCCA
  AAGCTGGTCACTGACTATAAGAATAAGAAATTCAATCTGGATGCACTGGTGACCCATACC
  CTGCCTTTTGACAAAATCAGTGAGGCATTTGACCTAATGAACCAAGGAAAAAGCATCCGA
  ACAATCCTCATCTTTTGAAGATGCCAGGAGCAATTCGGAATACTATCTGATTGAATGTGA
  ACCTGCCTGGTTAATTTATTACCTGATTTGATGAACCAAGGAAAGCCATGCGTTTAAACA
  AATATTTACATTTAATATGGGAACATAAAAGAGCTTTAAATATTATAGACTTTGTACCTG
  TTATATATATGAATATTCCCTATGTTAAATAATAATAATAACTAGTGTTTATGAATAGAA
  TCATATCATCTTTAGAAATTGTTTAAAATTAGTTCTGGGAAGTTGAAAGTGGGGAATGAA
  GAGATAATAAATAAAACTAGATTGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000670
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000670.3, NP_000661.2
• RefSeq Size: 1980 bp
• RefSeq ORF: 1143 bp
• Locus ID: 127
• UniProt ID: P08319
• Cytogenetics: 4q23
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism
• Gene Summary: This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of an alternate start codon compared to variant 1. It encodes isoform 2, which is shorter than and has a novel N-terminus compared to isoform 1.