ADH4 (NM_000670) Human Untagged Clone

CAT#: SC119754

ADH4 (untagged)-Human alcohol dehydrogenase 4 (class II), pi polypeptide (ADH4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADH4
• Synonyms: ADH-2; HEL-S-4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119754 sequence for NM_000670 edited (data generated by NextGen Sequencing)
  ATGGGCACCAAGGGCAAAGTTATTAAATGCAAAGCAGCCATCGCCTGGGAAGCAGGCAAG
  CCCCTTTGCATTGAAGAGGTTGAAGTAGCTCCCCCCAAGGCTCATGAAGTTCGCATTCAG
  ATCATTGCTACCTCCCTGTGCCATACTGATGCCACTGTTATCGATTCTAAATTTGAGGGC
  CTAGCTTTCCCAGTGATCGTTGGCCATGAGGCTGCAGGTATTGTGGAAAGTATTGGGCCA
  GGAGTGACCAACGTCAAACCAGGTGACAAAGTAATTCCACTTTATGCACCTCTATGTAGA
  AAATGCAAGTTTTGTCTGAGTCCACTCACAAATTTGTGTGGGAAAATCAGTAATCTCAAA
  AGTCCTGCTAGTGATCAACAACTAATGGAAGACAAAACCAGCAGGTTTACCTGCAAAGGA
  AAACCAGTTTACCATTTCTTTGGAACCAGTACATTCTCTCAGTACACTGTGGTGTCAGAT
  ATCAATCTTGCCAAAATAGATGATGATGCAAATTTAGAGAGAGTTTGTCTGCTTGGATGT
  GGGTTTTCAACTGGCTATGGGGCTGCAATCAACAATGCCAAGGTCACCCCTGGTTCGACT
  TGTGCTGTCTTTGGCCTAGGAGGTGTGGGTCTTTCTGCTGTAATGGGTTGTAAAGCAGCA
  GGAGCTTCCAGAATCATAGGTATTGACATCAACAGTGAGAAGTTTGTGAAGGCTAAAGCC
  CTGGGAGCCACTGACTGCCTCAATCCTAGAGACTTACATAAACCTATCCAGGAAGTTATC
  ATTGAATTGACCAAGGGAGGTGTGGATTTTGCCCTTGACTGTGCAGGTGGATCTGAAACC
  ATGAAAGCAGCCCTGGACTGTACAACCGCAGGCTGGGGATCATGTACTTTCATTGGAGTA
  GCTGCTGGTAGCAAAGGATTGACTGTTTTTCCAGAGGAGCTAATAATCGGCCGTACTATA
  AATGGAACATTCTTTGGTGGTTGGAAAAGTGTAGATTCTATCCCAAAGCTGGTCACTGAC
  TATAAGAATAAGAAATTCAATCTGGATGCACTGGTGACCCATACCCTGCCTTTTGACAAA
  ATCAGTGAGGCATTTGACCTAATGAACCAAGGAAAAAGCATCCGAACAATCCTCATCTTT
  TGA
  
  Clone variation with respect to NM_000670.3
  765 g=>t;925 a=>g;1120 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000670 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGCAGTTTCCCAAAAAAAAAGGGCACCAAGGGC
  AAAGTTATTAAATGCAAAGCAGCCATCGCCTGGGAAGCAGGCAAGCCCCTTTGCATTGAA
  GAGGTTGAAGTAGCTCCCCCCAAGGCTCATGAAGTTCGCATTCAGATCATTGCTACCTCC
  CTGTGCCATACTGATGCCACTGTTATCGATTCTAAATTTGAGGGCCTAGCTTTCCCAGTG
  ATCGTTGGCCATGAGGCTGCAGGTATTGTGGAAAGTATTGGGCCAGGAGTGACCAACGTC
  AAACCAGGTGACAAAGTAATTCCACTTTATGCACCTCTATGTAGAAAATGCAAGTTTTGT
  CTGAGTCCACTCACAAATTTGTGTGGGAAAATCAGTAATCTCAAAAGTCCTGCTAGTGAT
  CAACAACTAATGGAAGACAAAACCAGCAGGTTTACCTGCAAAGGAAAACCAGTTTACCAT
  TTCTTTGGAACCAGTACATTCTCTCAGTACACTGTGGTGTCAGATATCAATCTTGCCAAA
  ATAGATGATGATGCANATNTAGAGAGAGTTTGTCTGCTTGGATGTGGGTTTTCAACTGGC
  TATGGNGCTGNCATCAAACATGCCAAGGTCACCCCTGGNTCGACTTGTGCTGTCTTTGCC
  TANNGAGTGTGGGTCTTTCTGCTGTAATGGNTNGTAAAGCAGCAGGAGCTTCCAGAATCA
  TAGGTATTGACATCAACAGTGAGAAGTTTGTGAAGGCTAANAGCCCTGGACCACTGACTG
  CCTCCATCCTAGAGACTTACATANACCTATNCCAGAAAGTATCATTGAAATGACCAGGGN
  ANGGTGTGATNNTGCCCTTGACTGTGCNAGTGGATCTGAAACCATAAGGCAGNCCTGNAC
  TGTACACCGCNAGCTGGGATCATGTATTNCATTGNNATACTGCTGGTACAAAGATGACTT
  GTTTCCAAGACTAAAATCGNCGACTTTAATGGCTTCTTGGTGTTGAAAGGTAATCTACCC
  ACTGGCCTGCCTAAAAGAAATTATGGGTCTGGGACCACCTGCTTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000670 unedited
  TTTTTTTTTTTTTTTTTTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000670
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000670.3, NP_000661.2
• RefSeq Size: 1980 bp
• RefSeq ORF: 1143 bp
• Locus ID: 127
• UniProt ID: P08319
• Cytogenetics: 4q23
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism
• Gene Summary: This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an exon in the 5' coding region, which results in use of an alternate start codon compared to variant 1. It encodes isoform 2, which is shorter than and has a novel N-terminus compared to isoform 1.