ADH4 (NM_000670) Human Tagged ORF Clone

CAT#: RG204750

ADH4 (tGFP-tagged) - Human alcohol dehydrogenase 4 (class II), pi polypeptide (ADH4)

Product Images

Circular map for RG204750

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADH4
• Synonyms: ADH-2; HEL-S-4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204750 representing NM_000670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCAAGGGCAAAGTTATTAAATGCAAAGCAGCCATCGCCTGGGAAGCAGGCAAGCCCCTTTGCA
  TTGAAGAGGTTGAAGTAGCTCCCCCCAAGGCTCATGAAGTTCGCATTCAGATCATTGCTACCTCCCTGTG
  CCATACTGATGCCACTGTTATCGATTCTAAATTTGAGGGCCTAGCTTTCCCAGTGATCGTTGGCCATGAG
  GCTGCAGGTATTGTGGAAAGTATTGGGCCAGGAGTGACCAACGTCAAACCAGGTGACAAAGTAATTCCAC
  TTTATGCACCTCTATGTAGAAAATGCAAGTTTTGTCTGAGTCCACTCACAAATTTGTGTGGGAAAATCAG
  TAATCTCAAAAGTCCTGCTAGTGATCAACAACTAATGGAAGACAAAACCAGCAGGTTTACCTGCAAAGGA
  AAACCAGTTTACCATTTCTTTGGAACCAGTACATTCTCTCAGTACACTGTGGTGTCAGATATCAATCTTG
  CCAAAATAGATGATGATGCAAATTTAGAGAGAGTTTGTCTGCTTGGATGTGGGTTTTCAACTGGCTATGG
  GGCTGCAATCAACAATGCCAAGGTCACCCCTGGTTCGACTTGTGCTGTCTTTGGCCTAGGAGGTGTGGGT
  CTTTCTGCTGTAATGGGTTGTAAAGCAGCAGGAGCTTCCAGAATCATAGGTATTGACATCAACAGTGAGA
  AGTTTGTGAAGGCTAAAGCCCTGGGAGCCACTGACTGCCTCAATCCTAGAGACTTACATAAACCTATCCA
  GGAAGTTATCATTGAATTGACCAAGGGAGGTGTGGATTTTGCCCTTGACTGTGCAGGTGGATCTGAAACC
  ATGAAAGCAGCCCTGGACTGTACAACCGCAGGCTGGGGATCATGTACTTTCATTGGAGTAGCTGCTGGTA
  GCAAAGGATTGACTGTTTTTCCAGAGGAGCTAATAATCGGCCGTACTATAAATGGAACATTCTTTGGTGG
  TTGGAAAAGTGTAGATTCTATCCCAAAGCTGGTCACTGACTATAAGAATAAGAAATTCAATCTGGATGCA
  CTGGTGACCCATACCCTGCCTTTTGACAAAATCAGTGAGGCATTTGACCTAATGAACCAAGGAAAAAGCA
  TCCGAACAATCCTCATCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204750 representing NM_000670
  Red=Cloning site Green=Tags(s)
  
  MGTKGKVIKCKAAIAWEAGKPLCIEEVEVAPPKAHEVRIQIIATSLCHTDATVIDSKFEGLAFPVIVGHE
  AAGIVESIGPGVTNVKPGDKVIPLYAPLCRKCKFCLSPLTNLCGKISNLKSPASDQQLMEDKTSRFTCKG
  KPVYHFFGTSTFSQYTVVSDINLAKIDDDANLERVCLLGCGFSTGYGAAINNAKVTPGSTCAVFGLGGVG
  LSAVMGCKAAGASRIIGIDINSEKFVKAKALGATDCLNPRDLHKPIQEVIIELTKGGVDFALDCAGGSET
  MKAALDCTTAGWGSCTFIGVAAGSKGLTVFPEELIIGRTINGTFFGGWKSVDSIPKLVTDYKNKKFNLDA
  LVTHTLPFDKISEAFDLMNQGKSIRTILIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000670
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000670.3, NP_000661.2
• RefSeq Size: 1980 bp
• RefSeq ORF: 1143 bp
• Locus ID: 127
• UniProt ID: P08319
• Cytogenetics: 4q23
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism
• Gene Summary: This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]