DDX26 (INTS6) (NM_012141) Human Untagged Clone

CAT#: SC115535

INTS6 (untagged)-Human integrator complex subunit 6 (INTS6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX26
• Synonyms: DBI-1; DDX26; DDX26A; DICE1; HDB; INT6; Notchl2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115535 sequence for NM_012141 edited (data generated by NextGen Sequencing)
  ATGCCCATCTTACTGTTCCTGATAGACACGTCTGCCTCTATGAACCAGCGCAGCCATCTG
  GGCACCACCTACCTGGACACGGCCAAAGGCGCGGTAGAGACCTTCATGAAGCTCCGTGCC
  CGGGACCCTGCCAGCAGAGGAGACAGGTATATGCTGGTCACTTTCGAAGAGCCGCCCTAT
  GCTATCAAGGCTGGATGGAAAGAAAACCATGCAACGTTTATGAATGAATTGAAAAACCTT
  CAGGCTGAAGGACTTACGACTCTTGGCCAATCCCTAAGGACAGCTTTTGATTTATTAAAT
  TTAAATAGATTAGTAACTGGCATAGACAACTATGGGCAGGGAAGAAACCCTTTTTTCTTG
  GAGCCAGCAATAATTATCACAATTACTGATGGGAGCAAGTTGACTACCACCAGTGGAGTC
  CAGGATGAGCTTCATTTACCTCTTAATTCTCCTTTGCCTGGAAGTGAATTGACCAAGGAA
  CCTTTTCGTTGGGATCAGAGACTCTTTGCATTAGTGTTGCGGTTGCCTGGCACCATGTCA
  GTAGAATCAGAACAGTTGACAGGTGTGCCTTTAGATGACTCTGCAATCACACCAATGTGT
  GAAGTGACAGGCGGCCGTTCATATTCTGTGTGTTCTCCAAGAATGCTTAATCAGTGTCTG
  GAGTCCTTGGTGCAGAAAGTACAAAGTGGGGTGGTAATAAACTTTGAAAAAGCAGGACCA
  GATCCTTCCCCTGTAGAAGATGGGCAGCCAGATATATCAAGGCCTTTTGGATCTCAGCCT
  TGGCATAGCTGTCACAAACTCATATATGTCAGACCAAATCCTAAAACTGGGGTTCCTATA
  GGTCATTGGCCTGTTCCAGAGTCTTTTTGGCCAGATCAAAATTCGCCAACACTACCACCT
  CGTACATCTCATCCTGTAGTGAAGTTTTCCTGTACAGACTGTGAACCAATGGTTATTGAT
  AAACTTCCTTTTGACAAATATGAGTTGGAACCTTCACCACTGACTCAATTTATCCTGGAA
  AGGAAATCTCCTCAAACATGTTGGCAGGTGTACGTGAGCAATAGTGCAAAATACAGTGAA
  CTTGGTCATCCTTTTGGTTACTTGAAAGCCAGTACAGCACTGAACTGTGTCAACTTATTT
  GTGATGCCTTACAATTATCCAGTCCTTCTTCCCCTCTTAGATGACTTGTTTAAAGTGCAT
  AAAGCAAAACCAACATTGAAGTGGAGACAGTCATTTGAAAGTTATTTGAAGACAATGCCT
  CCCTACTATCTTGGGCCCTTGAAGAAAGCTGTTAGGATGATGGGAGCACCTAACCTAATA
  GCAGACAGTATGGAATATGGACTTAGTTACAGTGTCATTTCATACCTCAAAAAACTGAGT
  CAACAGGCCAAAATAGAATCTGATCGAGTCATTGGATCTGTAGGCAAAAAAGTAGTACAG
  GAGACTGGAATAAAAGTCCGGAGCCGATCACATGGTTTATCAATGGCATATAGGAAAGAT
  TTTCAACAACTCCTCCAGGGAATTTCAGAGGATGTCCCTCACAGACTGCTAGACCTTAAT
  ATGAAGGAATACACTGGGTTCCAAGTTGCTTTGCTGAATAAGGATTTGAAGCCACAGACA
  TTTAGAAATGCTTATGACATACCAAGACGAAATCTTTTGGATCACTTAACAAGAATGAGA
  TCTAATCTTTTGAAGAGCACTCGCAGATTTCTGAAAGGACAGGACGAAGATCAAGTGCAC
  AGTGTTCCTATAGCACAAATGGGGAACTACCAGGAATACCTCAAGCAAGTACCTTCTCCA
  CTAAGAGAACTTGATCCTGATCAGCCACGAAGGTTGCATACATTTGGCAACCCCTTTAAG
  CTGGATAAGAAGGGTATGATGATAGATGAAGCAGATGAATTTGTGGCTGGACCTCAAAAT
  AAACATAAACGACCCGGAGAACCAAATATGCAAGGGATCCCTAAAAGACGTCGGTGTATG
  TCTCCACTACTAAGAGGCAGACAGCAGAATCCTGTTGTAAACAATCATATTGGGGGAAAA
  GGACCACCTGCACCTACAACTCAAGCACAGCCAGATCTTATTAAACCTCTTCCTCTTCAT
  AAAATTTCAGAAACCACTAATGATTCGATAATACATGATGTGGTTGAAAATCATGTTGCA
  GACCAACTTTCATCAGACATTACACCAAATGCTATGGATACGGAATTTTCAGCATCTTCT
  CCAGCCAGTTTACTGGAACGGCCAACCAATCATATGGAGGCTCTTGGTCATGACCATTTA
  GGAACCAATGACCTCACTGTTGGTGGATTTTTAGAAAATCATGAGGAGCCAAGAGATAAA
  GAACAATGTGCTGAAGAGAACATACCAGCATCTTCACTCAACAAAGGAAAGAAATTGATG
  CATTGCAGAAGCCATGAAGAGGTCAATACTGAACTAAAAGCACAAATAATGAAAGAGATC
  CGAAAGCCAGGAAGAAAATATGAAAGAATCTTCACTTTACTGAAGCATGTGCAAGGCAGT
  TTACAAACAAGACTAATATTTTTACAAAATGTCATTAAAGAAGCATCAAGGTTTAAAAAA
  CGAATGCTAATAGAACAACTGGAGAACTTCTTGGATGAAATTCATCGAAGAGCCAATCAG
  ATCAACCATATTAATAGCAATTAA
  
  Clone variation with respect to NM_012141.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012141 unedited
  GGATATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGTGACACCGCC
  GGGGTGCGGAGGGAGTCCCTGAAACTTCTCCCAACAGCCCCAGGGCCAATCTTCCCTCAG
  CCCACTCCCCTCTTCGCCAGCTCCCAGGTCCCCACTCCTGTACCGGGGGTGGGGTGGGGA
  GCCAGGCCCGTCTCCCGCTGGGACACACACAGGGGCCGGGAGCGGGGACGGGACCCCCGA
  GGCGGGGGGGACGAACCGACAGACAGACGGCTGGGCGCCCGCCCCAGCGGGCAGGCAGGC
  AGGAGGAGGCGGAGGCGGGGGTACGACGGGGAGCACTGGGTCTGGGGAGTTTCCTCTCAA
  CTATCGGGGGAGAAACTCCCCGCAGCCGGAGGAAAGACCCAGACAGTGTTTTCCTCCCGG
  GGGCCGTGCTCCCCCGCCCCGCGTAGCGGCGGTCGCCGCCACCGGCGCCTCCACCTCTAC
  CATCTCCTCTTTCTCCACCACCTCGGGCCCCGGTGTCCCCGGCCAGCACTATGCCCATCT
  TACTGTTCCTGATAGACACGTCTGCCTCTATGAACCAGCGCAGCCATCTGGGCACCACCT
  ACCTGGACACGGCCAAAGGCGCGGTAGAGACCTTCATGAAGCTCCGTGCCCGGGACCCTG
  CCAGCAGAGGAGACAGGTATATGCTGGTCACTTTCGAAGAGCCGCCCTATGCTATCAAGG
  CTGGATGGAAAGAAAACCATGCAACGTTTATGAATGAATTGAAAAACCTTCAGGCTGANA
  GACTTACGACTCTTGGCCAATCCCTAAGGACAGCTNTTGATTTATTAAATTTAAATAGAT
  TAGTAACTGGCATAGACAACTATGGGCAGGNAAGAAACCCTNTTTNCTTGNNAGCCAGCA
  TATTATCACAATACTGATGGGAGCAGNTGACTACACAGTNGAGTCCCAGAGAGCTCATTA
  CCTCTAATCTCTGCCTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012141 unedited
  GGACAAAATCGCCCTCGATGTTTTTTATTAACCAGNACATTTATTGATGGAATAACAAAA
  TGCTTTTCTTAAATATTCTTCAAAATACATTTGTACAACTTACAATAATATATCCAAAAT
  AACTGTATATACAAAACATTACCTAGTTTTAATGTATGTGCTTTTTTCCCCAAAAATTAC
  AAAGNAACTNCTTTNTTTTTTTGTCCCCTCCCATCCTTAACAGAGGTGACAATTTTAAAG
  GTTGGTTTAAGAAAATAGAACCTGAGGGAAATTGACACGCAAGAGTCAATGACAAGCAAG
  AGATTTGGAGGAATATTTTTAGTTTGTTAAATTAAAAATGTTTTTTAGAGTGATACTTTT
  CACATTACAAAAATAAACCAAAATGAAGAAAAGGTCACTGTAAAATGATGGAAAAAGGAT
  CTGTAGATTTGCTTTTAGTATTTCAAATAGCCAACAATGCATGTCAGAAAATGAATGCAA
  GATCACAACAGNCTTGTATTTACTTTGTATTTGAAGAAGATAGTGAAATAAGTGGCCACA
  TTCTATTTTCTTTTAATTGCTATTAATATGGTTGATCTGATTGGCTCTTCGATGAATTTC
  ATCCAAGAAGTTCTCCAGTTGTTCTATTAGCATTCGTTTTTTAAACCTTGATGCTTCTTT
  AATGACATTTNTGAAAAATATTAGNCTTGGTTGTAAACTGCCTTGCACATGNCCTCAGTA
  AGNGAAGATTCTTTCATATTTCCTTCCTGGCTTTCGGATCTCTTTATTATTTGNGCTTTT
  ATTCAATATTGACCTCCTCATGGCTTCTGCAAGCATCAATTCTTTCCTTTGTGAATGAAA
  AGCTGGAATGTCTCTCCACAACATGNCTTTATCTCTGCCTCCCATGATTTCTAAAATCCC
  CACGGGAGGCATGGGCCTAAAGGCCTACCAAAACCCCTTGATGGTTGGCCGTCATAACGG
  CTGAAAAAACTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012141
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012141.1, NP_036273.1
• RefSeq Size: 3690 bp
• RefSeq ORF: 2664 bp
• Locus ID: 26512
• UniProt ID: Q9UL03
• Cytogenetics: 13q14.3
• Protein Families: Druggable Genome
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and is located in the critical region of loss of heterozygosity (LOH). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (a).