DDX26 (INTS6) (NM_012141) Human Tagged ORF Clone

CAT#: RG208036

INTS6 (tGFP-tagged) - Human integrator complex subunit 6 (INTS6), transcript variant 1

Product Images

Circular map for RG208036

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDX26
• Synonyms: DBI-1; DDX26; DDX26A; DICE1; HDB; INT6; Notchl2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208036 representing NM_012141
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATCTTACTGTTCCTGATAGACACGTCTGCCTCTATGAACCAGCGCAGCCATCTGGGCACCACCT
  ACCTGGACACGGCCAAAGGCGCGGTAGAGACCTTCATGAAGCTCCGTGCCCGGGACCCTGCCAGCAGAGG
  AGACAGGTATATGCTGGTCACTTTCGAAGAGCCGCCCTATGCTATCAAGGCTGGATGGAAAGAAAACCAT
  GCAACGTTTATGAATGAATTGAAAAACCTTCAGGCTGAAGGACTTACGACTCTTGGCCAATCCCTAAGGA
  CAGCTTTTGATTTATTAAATTTAAATAGATTAGTAACTGGCATAGACAACTATGGGCAGGGAAGAAACCC
  TTTTTTCTTGGAGCCAGCAATAATTATCACAATTACTGATGGGAGCAAGTTGACTACCACCAGTGGAGTC
  CAGGATGAGCTTCATTTACCTCTTAATTCTCCTTTGCCTGGAAGTGAATTGACCAAGGAACCTTTTCGTT
  GGGATCAGAGACTCTTTGCATTAGTGTTGCGGTTGCCTGGCACCATGTCAGTAGAATCAGAACAGTTGAC
  AGGTGTGCCTTTAGATGACTCTGCAATCACACCAATGTGTGAAGTGACAGGCGGCCGTTCATATTCTGTG
  TGTTCTCCAAGAATGCTTAATCAGTGTCTGGAGTCCTTGGTGCAGAAAGTACAAAGTGGGGTGGTAATAA
  ACTTTGAAAAAGCAGGACCAGATCCTTCCCCTGTAGAAGATGGGCAGCCAGATATATCAAGGCCTTTTGG
  ATCTCAGCCTTGGCATAGCTGTCACAAACTCATATATGTCAGACCAAATCCTAAAACTGGGGTTCCTATA
  GGTCATTGGCCTGTTCCAGAGTCTTTTTGGCCAGATCAAAATTCGCCAACACTACCACCTCGTACATCTC
  ATCCTGTAGTGAAGTTTTCCTGTACAGACTGTGAACCAATGGTTATTGATAAACTTCCTTTTGACAAATA
  TGAGTTGGAACCTTCACCACTGACTCAATTTATCCTGGAAAGGAAATCTCCTCAAACATGTTGGCAGGTG
  TACGTGAGCAATAGTGCAAAATACAGTGAACTTGGTCATCCTTTTGGTTACTTGAAAGCCAGTACAGCAC
  TGAACTGTGTCAACTTATTTGTGATGCCTTACAATTATCCAGTCCTTCTTCCCCTCTTAGATGACTTGTT
  TAAAGTGCATAAAGCAAAACCAACATTGAAGTGGAGACAGTCATTTGAAAGTTATTTGAAGACAATGCCT
  CCCTACTATCTTGGGCCCTTGAAGAAAGCTGTTAGGATGATGGGAGCACCTAACCTAATAGCAGACAGTA
  TGGAATATGGACTTAGTTACAGTGTCATTTCATACCTCAAAAAACTGAGTCAACAGGCCAAAATAGAATC
  TGATCGAGTCATTGGATCTGTAGGCAAAAAAGTAGTACAGGAGACTGGAATAAAAGTCCGGAGCCGATCA
  CATGGTTTATCAATGGCATATAGGAAAGATTTTCAACAACTCCTCCAGGGAATTTCAGAGGATGTCCCTC
  ACAGACTGCTAGACCTTAATATGAAGGAATACACTGGGTTCCAAGTTGCTTTGCTGAATAAGGATTTGAA
  GCCACAGACATTTAGAAATGCTTATGACATACCAAGACGAAATCTTTTGGATCACTTAACAAGAATGAGA
  TCTAATCTTTTGAAGAGCACTCGCAGATTTCTGAAAGGACAGGACGAAGATCAAGTGCACAGTGTTCCTA
  TAGCACAAATGGGGAACTACCAGGAATACCTCAAGCAAGTACCTTCTCCACTAAGAGAACTTGATCCTGA
  TCAGCCACGAAGGTTGCATACATTTGGCAACCCCTTTAAGCTGGATAAGAAGGGTATGATGATAGATGAA
  GCAGATGAATTTGTGGCTGGACCTCAAAATAAACATAAACGACCCGGAGAACCAAATATGCAAGGGATCC
  CTAAAAGACGTCGGTGTATGTCTCCACTACTAAGAGGCAGACAGCAGAATCCTGTTGTAAACAATCATAT
  TGGGGGAAAAGGACCACCTGCACCTACAACTCAAGCACAGCCAGATCTTATTAAACCTCTTCCTCTTCAT
  AAAATTTCAGAAACCACTAATGATTCGATAATACATGATGTGGTTGAAAATCATGTTGCAGACCAACTTT
  CATCAGACATTACACCAAATGCTATGGATACGGAATTTTCAGCATCTTCTCCAGCCAGTTTACTGGAACG
  GCCAACCAATCATATGGAGGCTCTTGGTCATGACCATTTAGGAACCAATGACCTCACTGTTGGTGGATTT
  TTAGAAAATCATGAGGAGCCAAGAGATAAAGAACAATGTGCTGAAGAGAACATACCAGCATCTTCACTCA
  ACAAAGGAAAGAAATTGATGCATTGCAGAAGCCATGAAGAGGTCAATACTGAACTAAAAGCACAAATAAT
  GAAAGAGATCCGAAAGCCAGGAAGAAAATATGAAAGAATCTTCACTTTACTGAAGCATGTGCAAGGCAGT
  TTACAAACAAGACTAATATTTTTACAAAATGTCATTAAAGAAGCATCAAGGTTTAAAAAACGAATGCTAA
  TAGAACAACTGGAGAACTTCTTGGATGAAATTCATCGAAGAGCCAATCAGATCAACCATATTAATAGCAA
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208036 representing NM_012141
  Red=Cloning site Green=Tags(s)
  
  MPILLFLIDTSASMNQRSHLGTTYLDTAKGAVETFMKLRARDPASRGDRYMLVTFEEPPYAIKAGWKENH
  ATFMNELKNLQAEGLTTLGQSLRTAFDLLNLNRLVTGIDNYGQGRNPFFLEPAIIITITDGSKLTTTSGV
  QDELHLPLNSPLPGSELTKEPFRWDQRLFALVLRLPGTMSVESEQLTGVPLDDSAITPMCEVTGGRSYSV
  CSPRMLNQCLESLVQKVQSGVVINFEKAGPDPSPVEDGQPDISRPFGSQPWHSCHKLIYVRPNPKTGVPI
  GHWPVPESFWPDQNSPTLPPRTSHPVVKFSCTDCEPMVIDKLPFDKYELEPSPLTQFILERKSPQTCWQV
  YVSNSAKYSELGHPFGYLKASTALNCVNLFVMPYNYPVLLPLLDDLFKVHKAKPTLKWRQSFESYLKTMP
  PYYLGPLKKAVRMMGAPNLIADSMEYGLSYSVISYLKKLSQQAKIESDRVIGSVGKKVVQETGIKVRSRS
  HGLSMAYRKDFQQLLQGISEDVPHRLLDLNMKEYTGFQVALLNKDLKPQTFRNAYDIPRRNLLDHLTRMR
  SNLLKSTRRFLKGQDEDQVHSVPIAQMGNYQEYLKQVPSPLRELDPDQPRRLHTFGNPFKLDKKGMMIDE
  ADEFVAGPQNKHKRPGEPNMQGIPKRRRCMSPLLRGRQQNPVVNNHIGGKGPPAPTTQAQPDLIKPLPLH
  KISETTNDSIIHDVVENHVADQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGF
  LENHEEPRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQIMKEIRKPGRKYERIFTLLKHVQGS
  LQTRLIFLQNVIKEASRFKKRMLIEQLENFLDEIHRRANQINHINSN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012141
• ORF Size: 2661 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012141.3
• RefSeq Size: 7465 bp
• RefSeq ORF: 2664 bp
• Locus ID: 26512
• UniProt ID: Q9UL03
• Cytogenetics: 13q14.3
• Protein Families: Druggable Genome
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and is located in the critical region of loss of heterozygosity (LOH). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2015]