Masp2 (NM_172043) Rat Tagged ORF Clone

CAT#: RR213700

Masp2 (Myc-DDK-tagged ORF) - Rat mannan-binding lectin serine peptidase 2 (Masp2), (10 ug)

Product Images

Circular map for RR213700

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Masp2
• Synonyms: MAp19; MASP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213700 representing NM_172043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTACTGATCGTCCTGGGTCTGCTTTGGAGTTTGGTGGCCACACTTTTGGGCTCCAAGTGGCCTG
  AGCCTGTATTCGGGCGCCTGGTGTCCCCTGGCTTCCCAGAGAAGTATGGCAACCATCAGGATCGATCCTG
  GACGCTGACTGCACCCCCTGGCTTCCGCCTGCGCCTCTACTTCACCCACTTCAACCTGGAACTCTCTTAC
  CGCTGCGAGTATGACTTTGTCAAGTTGACCTCAGGGACCAAGGTGCTAGCCACGCTGTGTGGGCAGGAGA
  GTACAGATACTGAGCGGGCACCTGGCAATGACACCTTCTACTCACTGGGTCCCAGCCTAAAGGTCACCTT
  CCACTCCGACTACTCCAATGAGAAGCCATTCACAGGATTTGAGGCCTTCTATGCAGCGGAGGATGTGGAT
  GAATGCAGAACATCCCTGGGAGACTCAGTCCCTTGTGACCATTATTGCCACAACTACCTGGGCGGCTACT
  ACTGCTCCTGCCGAGTGGGCTACATTCTGCACCAGAACAAGCATACCTGCTCAGCCCTTTGTTCAGGCCA
  GGTGTTCACTGGGAGGTCTGGCTTTCTCAGTAGCCCTGAGTACCCACAGCCATACCCCAAACTCTCCAGC
  TGCGCCTACAACATCCGCCTGGAGGAAGGCTTCAGTATCACCCTGGACTTCGTGGAGTCCTTTGATGTGG
  AGATGCACCCTGAAGCCCAGTGCCCCTACGACTCCCTCAAGATTCAAACAGACAAGAGGGAATACGGCCC
  GTTTTGTGGGAAGACGCTGCCCCCCAGGATTGAAACTGACAGCAACAAGGTGACCATTACCTTTACCACC
  GACGAGTCAGGGAACCACACAGGCTGGAAGATACACTACACAAGCACAGCACAGCCCTGCCCTGATCCAA
  CGGCGCCACCTAATGGTCACATTTCACCTGTGCAAGCCACGTATGTCCTGAAGGACAGCTTTTCTGTCTT
  CTGCAAGACTGGCTTCGAGCTTCTGCAAGGTTCTGTCCCCCTGAAGTCATTCACTGCTGTCTGTCAGAAA
  GATGGATCTTGGGACCGGCCGATACCAGAGTGCAGCATTATTGACTGTGGCCCTCCCGATGACCTACCCA
  ATGGCCACGTGGACTATATCACAGGCCCTGAAGTGACCACCTACAAAGCTGTGATTCAGTACAGCTGTGA
  AGAGACTTTCTACACAATGAGCAGCAATGGTAAATATGTGTGTGAGGCTGATGGATTCTGGACGAGCTCC
  AAAGGAGAAAAATCCCTCCCGGTTTGCAAGCCTGTCTGTGGACTGTCCACACACACTTCAGGAGGCCGTA
  TAATTGGAGGACAGCCTGCAAAGCCTGGTGACTTTCCTTGGCAAGTCTTGTTACTGGGTGAAACTACAGC
  AGCAGGTGCTCTTATACATGACGACTGGGTCCTAACAGCGGCTCATGCTGTATATGGGAAAACAGAGGCG
  ATGTCCTCCCTGGACATCCGCATGGGCATCCTCAAAAGGCTCTCCCCTCATTACACTCAAGCCTGGCCAG
  AGGCTGTCTTTATCCATGAAGGCTACACTCACGGAGCTGGTTTTGACAATGATATAGCACTGATTAAACT
  CAAGAACAAAGTCACAATCAACAGAAACATCATGCCGATTTGTCTACCAAGAAAAGAAGCTGCATCCTTA
  ATGAAAACAGACTTCGTTGGAACTGTGGCTGGCTGGGGGTTAACCCAGAAGGGGTTTCTTGCTAGAAACC
  TAATGTTTGTGGACATACCAATTGTTGACCACCAAAAATGTGCTACTGCGTATACAAAGCAGCCCTACCC
  AGGAGCAAAAGTGACTGTTAACATGCTCTGTGCTGGCCTAGACGCCGGTGGCAAGGACAGCTGCAGAGGT
  GACAGCGGAGGGGCATTAGTGTTTCTAGACAATGAAACACAGAGATGGTTTGTGGGAGGAATAGTTTCCT
  GGGGTTCTATTAACTGTGGGGGGTCAGAACAGTATGGGGTCTACACGAAAGTCACGAACTATATTCCCTG
  GATTGAGAACATAATAAATAATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213700 representing NM_172043
  Red=Cloning site Green=Tags(s)
  
  MRLLIVLGLLWSLVATLLGSKWPEPVFGRLVSPGFPEKYGNHQDRSWTLTAPPGFRLRLYFTHFNLELSY
  RCEYDFVKLTSGTKVLATLCGQESTDTERAPGNDTFYSLGPSLKVTFHSDYSNEKPFTGFEAFYAAEDVD
  ECRTSLGDSVPCDHYCHNYLGGYYCSCRVGYILHQNKHTCSALCSGQVFTGRSGFLSSPEYPQPYPKLSS
  CAYNIRLEEGFSITLDFVESFDVEMHPEAQCPYDSLKIQTDKREYGPFCGKTLPPRIETDSNKVTITFTT
  DESGNHTGWKIHYTSTAQPCPDPTAPPNGHISPVQATYVLKDSFSVFCKTGFELLQGSVPLKSFTAVCQK
  DGSWDRPIPECSIIDCGPPDDLPNGHVDYITGPEVTTYKAVIQYSCEETFYTMSSNGKYVCEADGFWTSS
  KGEKSLPVCKPVCGLSTHTSGGRIIGGQPAKPGDFPWQVLLLGETTAAGALIHDDWVLTAAHAVYGKTEA
  MSSLDIRMGILKRLSPHYTQAWPEAVFIHEGYTHGAGFDNDIALIKLKNKVTINRNIMPICLPRKEAASL
  MKTDFVGTVAGWGLTQKGFLARNLMFVDIPIVDHQKCATAYTKQPYPGAKVTVNMLCAGLDAGGKDSCRG
  DSGGALVFLDNETQRWFVGGIVSWGSINCGGSEQYGVYTKVTNYIPWIENIINNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172043
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172043.1, NP_742040.1
• RefSeq Size: 2095 bp
• RefSeq ORF: 2058 bp
• Locus ID: 64459
• UniProt ID: Q9JJS8
• Cytogenetics: 5q36
• MW: 75.6 kDa
• Gene Summary: member of the MBL/MASPs/Map19 complex of the mannan binding lectin pathway of complement activation; catalyzes the cleavage of complement component C4 [RGD, Feb 2006]