Masp2 (NM_172043) Rat Untagged Clone

CAT#: RN213700

Masp2 (untagged ORF) - Rat mannan-binding lectin serine peptidase 2 (Masp2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Masp2
• Synonyms: MAp19; MASP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213700 representing NM_172043
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTACTGATCGTCCTGGGTCTGCTTTGGAGTTTGGTGGCCACACTTTTGGGCTCCAAGTGGCCTG
  AGCCTGTATTCGGGCGCCTGGTGTCCCCTGGCTTCCCAGAGAAGTATGGCAACCATCAGGATCGATCCTG
  GACGCTGACTGCACCCCCTGGCTTCCGCCTGCGCCTCTACTTCACCCACTTCAACCTGGAACTCTCTTAC
  CGCTGCGAGTATGACTTTGTCAAGTTGACCTCAGGGACCAAGGTGCTAGCCACGCTGTGTGGGCAGGAGA
  GTACAGATACTGAGCGGGCACCTGGCAATGACACCTTCTACTCACTGGGTCCCAGCCTAAAGGTCACCTT
  CCACTCCGACTACTCCAATGAGAAGCCATTCACAGGATTTGAGGCCTTCTATGCAGCGGAGGATGTGGAT
  GAATGCAGAACATCCCTGGGAGACTCAGTCCCTTGTGACCATTATTGCCACAACTACCTGGGCGGCTACT
  ACTGCTCCTGCCGAGTGGGCTACATTCTGCACCAGAACAAGCATACCTGCTCAGCCCTTTGTTCAGGCCA
  GGTGTTCACTGGGAGGTCTGGCTTTCTCAGTAGCCCTGAGTACCCACAGCCATACCCCAAACTCTCCAGC
  TGCGCCTACAACATCCGCCTGGAGGAAGGCTTCAGTATCACCCTGGACTTCGTGGAGTCCTTTGATGTGG
  AGATGCACCCTGAAGCCCAGTGCCCCTACGACTCCCTCAAGATTCAAACAGACAAGAGGGAATACGGCCC
  GTTTTGTGGGAAGACGCTGCCCCCCAGGATTGAAACTGACAGCAACAAGGTGACCATTACCTTTACCACC
  GACGAGTCAGGGAACCACACAGGCTGGAAGATACACTACACAAGCACAGCACAGCCCTGCCCTGATCCAA
  CGGCGCCACCTAATGGTCACATTTCACCTGTGCAAGCCACGTATGTCCTGAAGGACAGCTTTTCTGTCTT
  CTGCAAGACTGGCTTCGAGCTTCTGCAAGGTTCTGTCCCCCTGAAGTCATTCACTGCTGTCTGTCAGAAA
  GATGGATCTTGGGACCGGCCGATACCAGAGTGCAGCATTATTGACTGTGGCCCTCCCGATGACCTACCCA
  ATGGCCACGTGGACTATATCACAGGCCCTGAAGTGACCACCTACAAAGCTGTGATTCAGTACAGCTGTGA
  AGAGACTTTCTACACAATGAGCAGCAATGGTAAATATGTGTGTGAGGCTGATGGATTCTGGACGAGCTCC
  AAAGGAGAAAAATCCCTCCCGGTTTGCAAGCCTGTCTGTGGACTGTCCACACACACTTCAGGAGGCCGTA
  TAATTGGAGGACAGCCTGCAAAGCCTGGTGACTTTCCTTGGCAAGTCTTGTTACTGGGTGAAACTACAGC
  AGCAGGTGCTCTTATACATGACGACTGGGTCCTAACAGCGGCTCATGCTGTATATGGGAAAACAGAGGCG
  ATGTCCTCCCTGGACATCCGCATGGGCATCCTCAAAAGGCTCTCCCCTCATTACACTCAAGCCTGGCCAG
  AGGCTGTCTTTATCCATGAAGGCTACACTCACGGAGCTGGTTTTGACAATGATATAGCACTGATTAAACT
  CAAGAACAAAGTCACAATCAACAGAAACATCATGCCGATTTGTCTACCAAGAAAAGAAGCTGCATCCTTA
  ATGAAAACAGACTTCGTTGGAACTGTGGCTGGCTGGGGGTTAACCCAGAAGGGGTTTCTTGCTAGAAACC
  TAATGTTTGTGGACATACCAATTGTTGACCACCAAAAATGTGCTACTGCGTATACAAAGCAGCCCTACCC
  AGGAGCAAAAGTGACTGTTAACATGCTCTGTGCTGGCCTAGACGCCGGTGGCAAGGACAGCTGCAGAGGT
  GACAGCGGAGGGGCATTAGTGTTTCTAGACAATGAAACACAGAGATGGTTTGTGGGAGGAATAGTTTCCT
  GGGGTTCTATTAACTGTGGGGGGTCAGAACAGTATGGGGTCTACACGAAAGTCACGAACTATATTCCCTG
  GATTGAGAACATAATAAATAATTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172043
• Insert Size: 2058 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172043.1, NP_742040.1
• RefSeq Size: 2095 bp
• RefSeq ORF: 2058 bp
• Locus ID: 64459
• UniProt ID: Q9JJS8
• Cytogenetics: 5q36
• Gene Summary: member of the MBL/MASPs/Map19 complex of the mannan binding lectin pathway of complement activation; catalyzes the cleavage of complement component C4 [RGD, Feb 2006]