Abhd6 (NM_001007680) Rat Tagged ORF Clone

CAT#: RR212804

Abhd6 (Myc-DDK-tagged ORF) - Rat abhydrolase domain containing 6 (Abhd6), (10 ug)

Product Images

Circular map for RR212804

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abhd6
• Synonyms: MGC94917
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212804 representing NM_001007680
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCGATGTGGTTAACATGTTTGTGATTGCGGGTGGGACCCTGGCCATTCCGATCCTGGCCTTCG
  TAGCTTCTTTCCTCCTTTGGCCCTCGGCGCTGATAAGAATCTATTATTGGTACTGGCGGAGGACGCTGGG
  CATGCAAGTCCGCTACGTGCACCACGAGGACTATCAGTTCTGTTACTCCTTCCGGGGCAGGCCAGGACAC
  AAGCCGTCCGTCCTTATGCTTCACGGGTTCTCCGCACACAAGGATATGTGGCTCAGTGTGGTCAAGTTCC
  TTCCCAAGAATCTGCACTTGGTCTGTGTGGACATGCCTGGACATGAAGGGACCACCCGTTCCTCCCTGGA
  TGACCTGTCCATAGTTGGGCAAGTGAAAAGGATACATCAGTTTGTAGAATGCCTTAAGCTGAACAAAAAG
  CCCTTTCACCTTATAGGCACCTCCATGGGTGGCAACGTGGCTGGAGTATACGCTGCTTACTATCCATCTG
  ACGTCTGCAGCCTGTCTCTCGTGTGCCCTGCTGGCCTGCAGTATTCAACTGACAATCGGTTTGTACAACG
  GCTCAAAGAGCTGGAGGACTCAGCCGCCACGCAGAAGATCCCTTTGATCCCATCCACCCCGGAAGAGATG
  AGCGAGATGCTGCAGCTCTGCTCGTACGTCCGCTTCAAGGTGCCCCAGCAGATCCTTCAAGGTCTTGTCG
  ACGTTCGCATCCCTCACAACAGCTTCTACCGGAAATTGTTTTTGGAAATCGTCAGTGAGAAATCCAGATA
  CTCTCTGCATGAGAACATGGACAAGATCAAGGTCCCAACACAGATCATTTGGGGAAAACAAGACCAGGTG
  CTTGATGTGTCGGGGGCAGACATATTAGCCAAGTCCATCACTAACTCCCAGGTAGAGGTTCTGGAAAACT
  GTGGCCATTCAGTGGTGATGGAGAGACCGAGGAAGACAGCCAAGCTCGTCGTCGACTTTTTAGCTTCTGT
  GCACAACCCAGACAACAACAAGAAGCTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212804 representing NM_001007680
  Red=Cloning site Green=Tags(s)
  
  MDLDVVNMFVIAGGTLAIPILAFVASFLLWPSALIRIYYWYWRRTLGMQVRYVHHEDYQFCYSFRGRPGH
  KPSVLMLHGFSAHKDMWLSVVKFLPKNLHLVCVDMPGHEGTTRSSLDDLSIVGQVKRIHQFVECLKLNKK
  PFHLIGTSMGGNVAGVYAAYYPSDVCSLSLVCPAGLQYSTDNRFVQRLKELEDSAATQKIPLIPSTPEEM
  SEMLQLCSYVRFKVPQQILQGLVDVRIPHNSFYRKLFLEIVSEKSRYSLHENMDKIKVPTQIIWGKQDQV
  LDVSGADILAKSITNSQVEVLENCGHSVVMERPRKTAKLVVDFLASVHNPDNNKKLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007680
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007680.1, NP_001007681.1
• RefSeq Size: 2225 bp
• RefSeq ORF: 1014 bp
• Locus ID: 305795
• UniProt ID: Q5XI64
• Cytogenetics: 15p14
• MW: 38.3 kDa
• Gene Summary: Lipase that preferentially hydrolysis medium-chain saturated monoacylglycerols including 2-arachidonoylglycerol (By similarity). Through 2-arachidonoylglycerol degradation may regulate endocannabinoid signaling pathways. Also has a lysophosphatidyl lipase activity with a preference for lysophosphatidylglycerol among other lysophospholipids (By similarity). Also able to degrade bis(monoacylglycero)phosphate (BMP) and constitutes the major enzyme for BMP catabolism. BMP, also known as lysobisphosphatidic acid, is enriched in late endosomes and lysosomes and plays a key role in the formation of intraluminal vesicles and in lipid sorting (By similarity).[UniProtKB/Swiss-Prot Function]