Arhgap29 (NM_001009405) Rat Tagged ORF Clone

CAT#: RR212783

Arhgap29 (Myc-DDK-tagged ORF) - Rat Rho GTPase activating protein 29 (Arhgap29), (10 ug)

Product Images

Circular map for RR212783

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap29
• Synonyms: B130017i01rik; RGD1306185
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212783 representing NM_001009405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGCATCAGAACCTCAATTCTGTAGACCTTCAGAACGCTGCAGAAACGCTCGCTTCGAAAGTGA
  AAGCTGTGAACTTTACAGAAGTTAATGACGAAAACAAAAACGATCTATTTCGAGAAGTCTTTTCCTCCAT
  TGAAACTCTGGCATTTACCTTTGGAAACATCCTCACAAACTTCCTTATGGGAGATGTAGGCAATGATTCG
  ATACTACGACTGCCTATTTCTCGAGAGAGTAAGTCCTTTGAAAACATTTCTATGGACTCAGTGGACTTAC
  CTCATGAAAAAGGAAACTTTTCTCCTATAGAACTAGACAACTTGCTGTTAAAGAACTCTGACTCTATAGA
  ACTGGCTTTGTCTTATGCTAAAACTTGGTCAAAATATACCAAGAACATAGTGTCCTGGGTTGAAAAAAAG
  CTCAACTTGGAATTGGAGTCCACTAGAAATATTGTAAAATTGGCAGAGGCAACTAGATCTAGCATTGGAA
  TACAGGAGTTTATGCCTTTGCAGTCTCTCTTTACCAACGCTCTCCTTAATGATATACACAGCAGTCACCT
  TTTACAACAGACAATCGCAGCTCTCCAAGCCAACAAATTCGTGCAGCCTCTACTTGGGAGGAAGAATGAA
  ATGGAGAAACAAAGGAAAGAAATAAAAGAACTTTGGAAGCAGCAGCAGAATAAATTGCTTGAAACAGAGA
  CGGCTCTTAAAAAGGCAAAACTGTTATGCATGCAACGGCAAGATGAATACGAAAAGGCAAAATCATCCAT
  GTTTCGAGCAGAAGAAGAGCAGCTGAGTTCAAGTGTTGGACTGGGAAAAAATCTCAACAAACTACTAGAA
  AAAAGGCGGAGATTGGAAGAAGAGGCGCTTCAAAAAGTAGAAGAAGCAAATGAGCACTACAAAGTCTGTG
  TAACAAATGTTGAAGAAAGACGGAATGATCTAGAAAATACGAAGCGAGAAATTTTAACACAGCTCCGGAC
  ACTTGTTTTCCAGTGTGACCTTACACTTAAAGCTGTAACAGTTAACCTCTTTCATATGCAGCAGCTTCAG
  GCTGCTTCCCTCGCCAACAGTTTACAGTCCCTCTGTGACAGTGCCAAACTCTATGATCCAGGTCAGGAGT
  ATAGTGAATTCGTGAAGGCTACAAGCTCAAGTGAATTAGAAGAAAAGGTTGACGGGAATGTAAACAAACA
  AATAGCCAGCAGTCCGCAGACATCTGGATATGAACCTGCTGACTCTTTAGAGGATGTTGCACGCCTCCCT
  GACAGCTGTCACAAACTTGAAGAGGACAGGTGTTCCAACAGTGCAGACATGACAGGTCCTTCTTTCATAA
  GATCGTGGAAGTTCGGGATGTTTAGTGACTCTGAGAGTACTGGAGGAAGCAGTGAGTCTAGATCTCTGGA
  TTCAGAATCTATAAGTCCAGGAGACTTTCATCGGAAACTTCCACGAACTCCATCCAGTGGAACCATGTCT
  TCTGCTGATGATCTAGATGAACGAGAGCCACCATCACCTTCAGAAGCTGGACCCAATTCCCTCGGAACAT
  TTAAGAAAACTTTGATGTCGAAGGCAGCTCTCACTCACAAGTTTCGCAAGTTGAGATCCCCCACAAAGTG
  CAGGGATTGTGAAGGCATCGTAATGTTCCCGGGCGTCGAGTGTGAAGAGTGTCTCCTTGTTTGTCACCGA
  AAGTGTTTGGAGAATTTAGTCATTGTTTGTGGTCATCAAAAACTTCAGGGGAAAATGCACATATTTGGAG
  CAGAATTCATACAAGTTGCAAAAAAGGAACCAGATGGCATCCCTTTCGTGCTAAAAATATGCGCCTCAGA
  AATTGAAAGTAGAGCCTTGTGTCTCCAGGGAATTTATCGTGTTTGTGGAAACAAAATAAAAACTGAAAAA
  CTGTGCCAAGCTTTGGAAAATGGAATGCACTTAGTAGACATTTCAGAATTCAGTTCACATGACATCTGTG
  ATGTCTTGAAATTATACCTGCGACAGCTTCCAGAACCATTTATTTTATTCAGATTGTACAAGGAATTTAT
  AGACCTTGCAAAAGAGATACAACATGTAAATGAAGAACAAGAGGCAAAAAAAGATAGCCCCGAAGACAAA
  AAGCACCCACACGTGAGCATAGAAATCAACCGCATCCTTCTGAGGAGCAAGGACCTGCTGCGACAGCTGC
  CAGCATCAAACTTCAACAGTCTTCATTACCTCATCGTCCATCTGAAGCGGGTGGTGGACCATGCAGAAGA
  GAACAAGATGAATTCTAAGAACTTGGGGGTGATATTTGGCCCAACGCTCATTAGGCCAAGGCCTACAACG
  GCTCCTGTCACCATCTCGTCCCTTGCTGAATATTCCAGTCAGGCACGAGTAGTAGAGTTCCTCATTACTT
  ACGCACAGAAGATCTTCGATGGGTCCCTCCAGCCTCAAGCTGGTGTCATAGCTAACACAGGTGCTATTGC
  ACCTCAAGTCGATCACGGGTGTCATCCAAAACCGCTCTTATCACCAGATGAGAGAGACTCGGATCATTCT
  TTGAAACAACTATTCTTCTCTTCAAAGGAAGATATCCGTACTATGGATTGTGAGAGCAAAACTTTTGAAT
  TAACTACATCATTTGAAGAATCAGAACGCAAACAAAATGCATTGGGAAAATGTGATGCTCCCATCCTTGA
  TAACAAAGTACATTTGCTTTTTGACCAAGAGCTTGAGTCAGCATCCCACAAGACGGAAGATACCTGTAAA
  AGCCCTAAGCTGCTCCTTCTGAGATCTGATAGGGTAGCAAATAGTGTGCAGAGACCGACTCCGAGGACCA
  GGCTAAGACCTGTAAGTCTGCCTGTGGATCGACTGCTGCTGCTTGCCGGGTCTCCTACTGAGAGAAGCAG
  CCGGAATACAGGAAACACAGACTCAGACAAGTTTGGCAAGAATGCCGCCTTTGAAGGACTCCATAGAAAG
  GACAACTCAAATACTACTTGTTCCAAAGTTAATGGCTTTGACCAGCAAAATGTACAGAAATCCTGGGACA
  AACAGAATGAGCGGAACAGTTTCACTGCCAAGACTACGGTGATTATCCCCAGTGCCTATGCCGAGAAGGG
  ATTGGCAGTGAGCACGGGGAACAACAGGGGCCATTCCAGTGGTGCTGCCCAGCCTAGTAAAGCACATGCA
  GACCCCGCCAGGTCTGCAAGAGACACGTCCGAGCACAGCTCCTCTGACTCCTGCCCTGTTGCTGCTGTCA
  GAGCACCCAGAACACTGCAGCCCCAGCACTGGACAACGTTTTACAAACCACCTAACCCCACCTTCAATGT
  CAGGGGCACTGAGGAGAAAACAGCATTCCCCTCAGCAGCTGTGCCTCCTGTCCTGGTGCATGCTCCCCAG
  AGTCATGTGGCAAAGTCAGACCCAGATTTGGAGGCCACATTGGCTTGTCCTGTGCAGACAAGTGGTCAAC
  CTAAAGAGAGCTCTGAGGAGCCCGGCCTGCCTGAGGGGACTCCAACCTGCCAGAGACCTCGACTAAAACG
  AATGCAGCAGTTTGAAGACCTTGAAGATGAAATCCCACAGTTTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212783 representing NM_001009405
  Red=Cloning site Green=Tags(s)
  
  MSKHQNLNSVDLQNAAETLASKVKAVNFTEVNDENKNDLFREVFSSIETLAFTFGNILTNFLMGDVGNDS
  ILRLPISRESKSFENISMDSVDLPHEKGNFSPIELDNLLLKNSDSIELALSYAKTWSKYTKNIVSWVEKK
  LNLELESTRNIVKLAEATRSSIGIQEFMPLQSLFTNALLNDIHSSHLLQQTIAALQANKFVQPLLGRKNE
  MEKQRKEIKELWKQQQNKLLETETALKKAKLLCMQRQDEYEKAKSSMFRAEEEQLSSSVGLGKNLNKLLE
  KRRRLEEEALQKVEEANEHYKVCVTNVEERRNDLENTKREILTQLRTLVFQCDLTLKAVTVNLFHMQQLQ
  AASLANSLQSLCDSAKLYDPGQEYSEFVKATSSSELEEKVDGNVNKQIASSPQTSGYEPADSLEDVARLP
  DSCHKLEEDRCSNSADMTGPSFIRSWKFGMFSDSESTGGSSESRSLDSESISPGDFHRKLPRTPSSGTMS
  SADDLDEREPPSPSEAGPNSLGTFKKTLMSKAALTHKFRKLRSPTKCRDCEGIVMFPGVECEECLLVCHR
  KCLENLVIVCGHQKLQGKMHIFGAEFIQVAKKEPDGIPFVLKICASEIESRALCLQGIYRVCGNKIKTEK
  LCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQHVNEEQEAKKDSPEDK
  KHPHVSIEINRILLRSKDLLRQLPASNFNSLHYLIVHLKRVVDHAEENKMNSKNLGVIFGPTLIRPRPTT
  APVTISSLAEYSSQARVVEFLITYAQKIFDGSLQPQAGVIANTGAIAPQVDHGCHPKPLLSPDERDSDHS
  LKQLFFSSKEDIRTMDCESKTFELTTSFEESERKQNALGKCDAPILDNKVHLLFDQELESASHKTEDTCK
  SPKLLLLRSDRVANSVQRPTPRTRLRPVSLPVDRLLLLAGSPTERSSRNTGNTDSDKFGKNAAFEGLHRK
  DNSNTTCSKVNGFDQQNVQKSWDKQNERNSFTAKTTVIIPSAYAEKGLAVSTGNNRGHSSGAAQPSKAHA
  DPARSARDTSEHSSSDSCPVAAVRAPRTLQPQHWTTFYKPPNPTFNVRGTEEKTAFPSAAVPPVLVHAPQ
  SHVAKSDPDLEATLACPVQTSGQPKESSEEPGLPEGTPTCQRPRLKRMQQFEDLEDEIPQFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009405
• ORF Size: 3546 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009405.1, NP_001009405.1
• RefSeq Size: 4076 bp
• RefSeq ORF: 3549 bp
• Locus ID: 310833
• UniProt ID: Q5PQJ5
• Cytogenetics: 2q41
• MW: 132.3 kDa
• Gene Summary: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho (By similarity). In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis (By similarity).[UniProtKB/Swiss-Prot Function]