Arhgap29 (NM_001009405) Rat Untagged Clone

CAT#: RN212783

Arhgap29 (untagged ORF) - Rat Rho GTPase activating protein 29 (Arhgap29), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap29
• Synonyms: B130017i01rik; RGD1306185
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212783 representing NM_001009405
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGCATCAGAACCTCAATTCTGTAGACCTTCAGAACGCTGCAGAAACGCTCGCTTCGAAAGTGA
  AAGCTGTGAACTTTACAGAAGTTAATGACGAAAACAAAAACGATCTATTTCGAGAAGTCTTTTCCTCCAT
  TGAAACTCTGGCATTTACCTTTGGAAACATCCTCACAAACTTCCTTATGGGAGATGTAGGCAATGATTCG
  ATACTACGACTGCCTATTTCTCGAGAGAGTAAGTCCTTTGAAAACATTTCTATGGACTCAGTGGACTTAC
  CTCATGAAAAAGGAAACTTTTCTCCTATAGAACTAGACAACTTGCTGTTAAAGAACTCTGACTCTATAGA
  ACTGGCTTTGTCTTATGCTAAAACTTGGTCAAAATATACCAAGAACATAGTGTCCTGGGTTGAAAAAAAG
  CTCAACTTGGAATTGGAGTCCACTAGAAATATTGTAAAATTGGCAGAGGCAACTAGATCTAGCATTGGAA
  TACAGGAGTTTATGCCTTTGCAGTCTCTCTTTACCAACGCTCTCCTTAATGATATACACAGCAGTCACCT
  TTTACAACAGACAATCGCAGCTCTCCAAGCCAACAAATTCGTGCAGCCTCTACTTGGGAGGAAGAATGAA
  ATGGAGAAACAAAGGAAAGAAATAAAAGAACTTTGGAAGCAGCAGCAGAATAAATTGCTTGAAACAGAGA
  CGGCTCTTAAAAAGGCAAAACTGTTATGCATGCAACGGCAAGATGAATACGAAAAGGCAAAATCATCCAT
  GTTTCGAGCAGAAGAAGAGCAGCTGAGTTCAAGTGTTGGACTGGGAAAAAATCTCAACAAACTACTAGAA
  AAAAGGCGGAGATTGGAAGAAGAGGCGCTTCAAAAAGTAGAAGAAGCAAATGAGCACTACAAAGTCTGTG
  TAACAAATGTTGAAGAAAGACGGAATGATCTAGAAAATACGAAGCGAGAAATTTTAACACAGCTCCGGAC
  ACTTGTTTTCCAGTGTGACCTTACACTTAAAGCTGTAACAGTTAACCTCTTTCATATGCAGCAGCTTCAG
  GCTGCTTCCCTCGCCAACAGTTTACAGTCCCTCTGTGACAGTGCCAAACTCTATGATCCAGGTCAGGAGT
  ATAGTGAATTCGTGAAGGCTACAAGCTCAAGTGAATTAGAAGAAAAGGTTGACGGGAATGTAAACAAACA
  AATAGCCAGCAGTCCGCAGACATCTGGATATGAACCTGCTGACTCTTTAGAGGATGTTGCACGCCTCCCT
  GACAGCTGTCACAAACTTGAAGAGGACAGGTGTTCCAACAGTGCAGACATGACAGGTCCTTCTTTCATAA
  GATCGTGGAAGTTCGGGATGTTTAGTGACTCTGAGAGTACTGGAGGAAGCAGTGAGTCTAGATCTCTGGA
  TTCAGAATCTATAAGTCCAGGAGACTTTCATCGGAAACTTCCACGAACTCCATCCAGTGGAACCATGTCT
  TCTGCTGATGATCTAGATGAACGAGAGCCACCATCACCTTCAGAAGCTGGACCCAATTCCCTCGGAACAT
  TTAAGAAAACTTTGATGTCGAAGGCAGCTCTCACTCACAAGTTTCGCAAGTTGAGATCCCCCACAAAGTG
  CAGGGATTGTGAAGGCATCGTAATGTTCCCGGGCGTCGAGTGTGAAGAGTGTCTCCTTGTTTGTCACCGA
  AAGTGTTTGGAGAATTTAGTCATTGTTTGTGGTCATCAAAAACTTCAGGGGAAAATGCACATATTTGGAG
  CAGAATTCATACAAGTTGCAAAAAAGGAACCAGATGGCATCCCTTTCGTGCTAAAAATATGCGCCTCAGA
  AATTGAAAGTAGAGCCTTGTGTCTCCAGGGAATTTATCGTGTTTGTGGAAACAAAATAAAAACTGAAAAA
  CTGTGCCAAGCTTTGGAAAATGGAATGCACTTAGTAGACATTTCAGAATTCAGTTCACATGACATCTGTG
  ATGTCTTGAAATTATACCTGCGACAGCTTCCAGAACCATTTATTTTATTCAGATTGTACAAGGAATTTAT
  AGACCTTGCAAAAGAGATACAACATGTAAATGAAGAACAAGAGGCAAAAAAAGATAGCCCCGAAGACAAA
  AAGCACCCACACGTGAGCATAGAAATCAACCGCATCCTTCTGAGGAGCAAGGACCTGCTGCGACAGCTGC
  CAGCATCAAACTTCAACAGTCTTCATTACCTCATCGTCCATCTGAAGCGGGTGGTGGACCATGCAGAAGA
  GAACAAGATGAATTCTAAGAACTTGGGGGTGATATTTGGCCCAACGCTCATTAGGCCAAGGCCTACAACG
  GCTCCTGTCACCATCTCGTCCCTTGCTGAATATTCCAGTCAGGCACGAGTAGTAGAGTTCCTCATTACTT
  ACGCACAGAAGATCTTCGATGGGTCCCTCCAGCCTCAAGCTGGTGTCATAGCTAACACAGGTGCTATTGC
  ACCTCAAGTCGATCACGGGTGTCATCCAAAACCGCTCTTATCACCAGATGAGAGAGACTCGGATCATTCT
  TTGAAACAACTATTCTTCTCTTCAAAGGAAGATATCCGTACTATGGATTGTGAGAGCAAAACTTTTGAAT
  TAACTACATCATTTGAAGAATCAGAACGCAAACAAAATGCATTGGGAAAATGTGATGCTCCCATCCTTGA
  TAACAAAGTACATTTGCTTTTTGACCAAGAGCTTGAGTCAGCATCCCACAAGACGGAAGATACCTGTAAA
  AGCCCTAAGCTGCTCCTTCTGAGATCTGATAGGGTAGCAAATAGTGTGCAGAGACCGACTCCGAGGACCA
  GGCTAAGACCTGTAAGTCTGCCTGTGGATCGACTGCTGCTGCTTGCCGGGTCTCCTACTGAGAGAAGCAG
  CCGGAATACAGGAAACACAGACTCAGACAAGTTTGGCAAGAATGCCGCCTTTGAAGGACTCCATAGAAAG
  GACAACTCAAATACTACTTGTTCCAAAGTTAATGGCTTTGACCAGCAAAATGTACAGAAATCCTGGGACA
  AACAGAATGAGCGGAACAGTTTCACTGCCAAGACTACGGTGATTATCCCCAGTGCCTATGCCGAGAAGGG
  ATTGGCAGTGAGCACGGGGAACAACAGGGGCCATTCCAGTGGTGCTGCCCAGCCTAGTAAAGCACATGCA
  GACCCCGCCAGGTCTGCAAGAGACACGTCCGAGCACAGCTCCTCTGACTCCTGCCCTGTTGCTGCTGTCA
  GAGCACCCAGAACACTGCAGCCCCAGCACTGGACAACGTTTTACAAACCACCTAACCCCACCTTCAATGT
  CAGGGGCACTGAGGAGAAAACAGCATTCCCCTCAGCAGCTGTGCCTCCTGTCCTGGTGCATGCTCCCCAG
  AGTCATGTGGCAAAGTCAGACCCAGATTTGGAGGCCACATTGGCTTGTCCTGTGCAGACAAGTGGTCAAC
  CTAAAGAGAGCTCTGAGGAGCCCGGCCTGCCTGAGGGGACTCCAACCTGCCAGAGACCTCGACTAAAACG
  AATGCAGCAGTTTGAAGACCTTGAAGATGAAATCCCACAGTTTGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009405
• Insert Size: 3549 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009405.1, NP_001009405.1
• RefSeq Size: 4076 bp
• RefSeq ORF: 3549 bp
• Locus ID: 310833
• UniProt ID: Q5PQJ5
• Cytogenetics: 2q41
• Gene Summary: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho (By similarity). In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis (By similarity).[UniProtKB/Swiss-Prot Function]