Shank1 (NM_031751) Rat Tagged ORF Clone

CAT#: RR210818

Shank1 (Myc-DDK-tagged ORF) - Rat SH3 and multiple ankyrin repeat domains 1 (Shank1), (10 ug)

Product Images

Circular map for RR210818

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shank1
• Synonyms: Shank1a; Spank1; Sstrip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210818 representing NM_031751
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCACAGCCCCGCGACAAGCGAGGACGAGGAACGCCACAGTGCCAGCGAGTGTCCCGAGGGGGGCT
  CAGAATCGGACAGCTCCCCAGACGGACCAGGAAGAGGCCCCCAGGGTACTCGGGGCCGGGGCAGCGGAGC
  ACCTGGTAACCTGGCCTCTACCCGAGGCCTCCAGGGACGCTCCATGTCTGTCCCTGACGACGCCCACTTC
  AGCATGATGGTTTTCAGGATTGGCATCCCTGACTTGCACCAGACGAAATGCCTACGCTTCAACCCGGATG
  CTACCATCTGGACAGCCAAACAGCAAGTACTCTGTGCCCTGAGCGAGAGTCTACAGGATGTGCTAAACTA
  TGGACTGTTCCAGCCAGCCACCTCAGGCCGGGATGCCAACTTCCTGGAGGAAGAGAGGCTTCTAAGAGAG
  TACCCTCAGTCCTTTGAGAAAGGGGTACCCTACCTGGAGTTCCGCTACAAGACCCGAGTCTACAAACAGA
  CCAACCTGGATGAGAAACAGCTGGCCAAACTGCACACGAAGACGGGGTTGAAGAAGTTCCTTGAATATGT
  GCAGCTCGGGACGTCTGACAAGGTGGCGAGGCTGCTGGACAAGGGGCTGGACCCCAATTACCACGACTCA
  GATTCAGGAGAGACACCTTTGACCCTGGCGGCCCAGACTGAAGGCTCAGTAGAGGTGATTCGGACGCTGT
  GCCTGGGCGGGGCGCACATAGACTTCAGGGCCCGGGATGGCATGACAGCGCTGCACAAAGCTGCATGTGC
  TAGGCACTGCCTGGCACTCACGGCACTTCTGGACCTTGGGGGCTCCCCCAACTACAAGGATCGACGAGGG
  CTGACCCCCCTGTTCCACACAGCTATGGTTGGGGGAGACCCCCGCTGTTGTGAGCTACTTCTGTACAACA
  GGGCCCAGCTGGGCATTGCAGATGAGAACGGCTGGCAGGAAATCCACCAGGCCTGCCAACGAGGGCACTC
  TCAGCACCTGGAACACCTGCTCTTCTATGGGGCTGAGCCTGGAGCCCAGAATGCCTCAGGAAACACGGCC
  CTGCACATCTGTGCTCTCTACAACAAGGAGACCTGTGCCAGGATCCTCCTTTATCGCGGAGCAAACAAGG
  ATGTGAAAAATAACAACGGACAGACACCCTTCCAGGTGGCTGTGATTGCTGGGAATTTTGAGCTGGGGGA
  ACTGATTCGTAACCATCGGGAACAGGATGTGGTACCCTTCCAGGAGTCCCCCAAGTATGCAGCCCGGCGT
  CGGGGACCCCCAGGCGCAGGGCTGACTGTACCCCCAGCGCTGCTGCGGGCTAACAGCGACACGAGCATGG
  CGCTGCCTGATTGGATGGTGTTTTCGGCCCCAGGGGCCTCATCCTCTGGGACCCCTGGCCCTACCTCAGG
  ACCCCAGGGCCAGTCACAGCCCTCGGCCCCCAGCACCAAGCTCAGTAGTGGGACCCTCAGAAGTGCCAGC
  AGCCCCCGAGGTGCCCGGGCACGCTCCCCATCCCGGGGGAGACATCCTGAAGATGCCAAGAGACAACCTC
  GAGGCCGACCCAGCTCCAGCGGGACACCCCGAGATGGGCCGGCTGGGGGAACTGGGGGCTCAGGGGGCCC
  TGGGGGCTCCCTGGGTAGCAGAGGCAGACGTAGGAAGCTCTATTCAGCGGTACCTGGACGCTCCTTCATG
  GCGGTGAAGTCATACCAGGCGCAAGGCGAGGGGGAGATCTCTCTGAGTAAAGGCGAAAAGATCAAAGTGC
  TCAGCATCGGGGAAGGAGGCTTCTGGGAAGGCCAGGTCAAAGGTCGAGTTGGCTGGTTCCCTTCTGACTG
  CCTAGAAGAGGTGGCAAACCGTTCCCAGGAGGGAAGACAAGAAAGCCGAAGTGACAAGGCAAAGAGACTC
  TTCAGGCATTACACAGTGGGCTCTTACGACAGCTTCGATGCCCCAAGTCTGATCGATGGGATTGACTCAG
  GGAGTGATTACATCATCAAGGAGAAGACAGTCTTACTGCAGAAGAAGGACAGTGAGGGGTTCGGGTTCGT
  GCTCCGGGGGGCCAAGGCGCAGACCCCCATCGAGGAATTCACCCCCACCCCGGCCTTCCCGGCGCTGCAG
  TACCTCGAGTCTGTGGACGAGGGTGGCGTGGCATGGCGGGCTGGACTACGGATGGGAGACTTCCTCATCG
  AGGTGAATGGACAGAATGTAGTGAAGGTTGGCCACCGCCAGGTGGTCAACATGATCCGACAGGGAGGCAA
  CACACTGATGGTCAAGGTGGTGATGGTGACCAGACACCCAGACATGGACGAGGCTGTCCACAAGAAAGCA
  TCCCAGCAGGCTAAGCGGCTCCCGCCCCCAGCCATCTCCCTGCGTTCCAAGTCTATGACCTCAGAGCTGG
  AGGAAATGGTCTCCCCCTGGAAGAAGAAAATTGAATACGAGCAACAGCCCGCGGCAGTGCCCAGCATGGA
  GAAAAAGAGGACTGTGTATCAGATGGCACTCAACAAACTGGATGAGATCTTGGCCGCTGCTCAGCAGACC
  ATCAGCGCAAGTGAGAGTCCTGGGCCTGGTGGCCTGGCATCCCTGGGCAAGCACCGACCCAAAGGATTCT
  TTGCCACTGAGTCGAGCTTTGATCCTCACCACCGCTCCCAGCCAAGTTATGACCGGCCTTCTTTCCTGCC
  TCCTGGACCCGGCCTTATGCTCCGGCAGAAATCTATCGGGGCTGCGGAAGACGACAGACCCTACCTAGCA
  CCCCCAGCCATGAAATTCAGTCGTAGCCTGTCAGTCCCCGGTTCAGAGGACATACCCCCACCACCCACTA
  CATCACCACCAGAGCCGCCCTATAGCACACCTCCGGCCCCCTCCTCCTCCGGCCGCCTCACTCCCTCGCC
  TCGGGGAGGGCCCTTTAACCCCAGTTCTGGGGGTCCCCTTCCCGCCTCCTCCCCTTCATCTTTCGATGGG
  CCATCTCCTCCTGACACCCGTGGTGGGGGCCGAGAAAAGAGCCTATATCACAGCGCAGCCCTGCCCCCTG
  CCCACCACCATCCACCTCATCACCACCATCATCACGCGCCACCTCCCCAGCCCCACCATCACCATGCACA
  TCCTCCTCACCCACCGGAGATGGAGACAGGAGGTTCCCCCGACGATCCCCCACCTCGATTGGCCCTGGGA
  CCCCAGCCCAGCCTTCGAGGCTGGCGAGGTGGAGGACCCAGCCCGACCTCGGGGGCCCCATCACCTTCCC
  ACCACAGTAGCAGCGGAGGCAGCAGCGGCCCTACTCAGGCTCCCGCCCTGCGCTACTTCCAACTGCCCCC
  GAGGGCGGCCAGTGCGGCTATGTATGTACCAGCACGTTCCGGCCGCGGTCGCAAGGGCCCACTGGTCAAG
  CAGACCAAAGTGGAAGGCGAGCCCCAAAAGGGCAGCATCCCTTCCGCGTCCTCTCCAACATCCCCGGCGT
  TGCCTCGGTCTGAACCACCTCCGGCCGGGCCGTCGGAGAAAAATTCCATCCCTATCCCGACCATCATCAT
  CAAAGCCCCATCTACCAGTAGCAGCGGCCGCAGCAGTCAGGGAAGCAGCACAGAGGCTGAGCCCCCCACC
  CAGCCCGATGGCGCGGGCGGCGGCGGATCCTCGCCTAGCCCTGCACCTGCCACGTCCCCTGTGCCGCCAT
  CCCCGTCGCCCGTGCCCACTCCCGCGTCACCCAGCGGCCCGGCCACCCTGGATTTCACTAGCCAGTTTGG
  AGCAGCCCTAGTAGGCGCGGCGCGGAGGGAGGGAGGCTGGCAGAACGAAGCCCGGCGGCGGTCCACGCTG
  TTCCTGTCCACCGACGCAGGAGATGAGGATGGAGGCGACAGCGGGCTGGGCCCGGGGGGGCCCCCGGGTC
  CCCGGCTGCGCCATTCCAAATCCATTGACGAAGGCATGTTCTCCGCAGAGCCGTACCTCCGGCTGGAGTC
  GGGGGGCAGCAGTGGGGGCTACGGGGCCTACGCAGCGGGCAGCCGAGCCTACGGGGGCAGCGGGAGCAGC
  AGCGCCTTCACCAGCTTCCTCCCCCCGCGGCCCCTGGTGCATCCGCTGACCGGCAAAGCCTTGGATCCCG
  CCTCTCCGCTGGGGTTGGCCTTGGCCGCCCGGGAGCGGGCCTTGAAGGAGTCCTCGGAGGGTGGGGGCAC
  CCCTCAGCCGCCCCCGAGACCTCCATCACCACGCTACGACGCCCCACCGCCTACCCTTCACCACCACTCG
  CCCCATTCACCCCATTCGCCACATGCGCGTCACGAGCCAGTGTTGCGCCTCTGGGGGGACCCCGCGCGCC
  GGGAGCTGGGGTACCGGGCAGGCCTAGGGAGTCAGGAGAAGGCCCTTACCGCCAGTCCACCTGCAGCTCG
  CCGCTCCCTACTGCACCGTCTGCCCCCGACGGCTCCCGGGGTCGGGCCGCTCCTGCTGCAGCTGGGGCCG
  GAGCCCCCAACTCCCCATCCTGGGGTGAGCAAGGCCTGGCGCACAGCAGCCCCGGAGGAGCCGGAGCGGC
  TGCCGCTGCACGTGCGATTCCTTGAGAATTGCCAAGCCCGGCCTCCCCCAGCTGGCACGAGAGGGTCCTC
  TACTGAAGATGGGCCTGGGGTCCCGCCTCCCAGCCCTCGCAGAGTCTTGCCAACCTCTCCCACCTCCCCA
  CGAGGCAACGAGGAGAACGGCCTTCCGCTACTGGTCCTGCCACCGCCAGCCCCTTCGGTGGATGTGGACG
  ACGGCGAGTTCTTGTTTGCGGAGCCGCTGCCTCCACCCCTGGAGTTTTCCAATAGTTTCGAAAAGCCAGA
  GTCGCCTCTCACACCCGGGCCTCCTCACCCACTCCCTGACCCTCCCTCCCCGGCTACCCCTCTACCCGCA
  GCCCCACCTCCCGCTGTAGCCGCAGCCCCTCCCACCTTGGACTCCACAGCATCCAGCCTGACCTCTTACG
  ACAGCGAAGTGGCCACACTGACCCAGGGGGCTCCCGCGGCTCCTGGGGATCCTCCAGCTCCAGGCCCGCC
  GGCCCCGGCAGCCCCAGCTCCCCCAGCACCGCAGCCAGGCCCTGATCCTCCACCTGGCACAGATTCTGGA
  ATTGAGGAGGTGGACAGTCGGAGTAGCAGTGATCACCCGCTGGAGACCATCAGCAGCGCATCCACACTCA
  GCAGCTTGTCCGCCGAAGGAGGAGGCAATACAGGGGGCGTGGCTGGGGGCGGGGCCGGGGTGGCCAGTGG
  GACGGAGCTCCTGGACACCTACGTTGCCTACCTGGATGGCCAGGCCTTCGGGGGCAGTGGAACTCCTGGC
  CCACCATATCCTCCCCAGCTCATGACTCCTTCTAAGCTCCGGGGCAGGGCGCTTGGGACTAGTGGAAATT
  TAAGACCTGGTCCCAGTGGGGGACTCCGAGATCCTGTCACCCCCACCAGCCCCACTGTCTCTGTGACCGG
  AGCTGGAACAGATGGGCTACTGGCCTTGAGTGCTTGCCCTGGACCCTCAACGGCAGGTGTGGCAGGGGGT
  CCCGTGGCAGTTGAGCCAGAAGTCCCGCCTGTACCTTTGCCCGCTGCCTCATCCCTGCCTCGGAAGCTAT
  TACCGTGGGAGGAGGGTCCGGGACCACCACCACCACCCCTGCCAGGGCCCCTGTCCCAGCCTCAGGCCTC
  TGCCTTGGCCACGGTCAAAGCCAGCATCATCAGCGAACTCAGCTCCAAGCTCCAGCAGTTTGGTGGGTCC
  TCTACCGCAGGAGGAGCTCTGCCTTGGGCTCGGGGAGGCAGCGGGGGCAGCACAGACAGCCACCACGGAG
  GAGCCAGTTACATCCCAGAGAGGACGTCCTCCCTGCAGCGGCAGAGGCTCTCTGAAGACTCCCAGACCTC
  TCTCCTCTCCAAACCCAGCAGCAGCATCTTTCAGAACTGGCCTAAACCCCCTCTGCCACCACTCCCCACT
  GGGTCTGGGGTCTCCTCTTCAACAGCTGCAGCCCCGGGAGCTACATCCCCTTCAGCCTCATCTGCCTCAG
  CTTCCACCAGACACCTGCAAGGTGTGGAATTTGAGATGCGGCCTCCGCTGCTCCGCCGGGCACCCAGCCC
  TTCGTTGCTGCCTGCTTCGGATCACAAGGTCAGCCCAGCGCCCAGGCCTTCCTCTTTGCCCATCCTGCCT
  TCAGGACCCATCTACCCGGGCCTCTTCGACATCCGTAGCTCTCCAACCGGAGGGGCGGGAGGCTCGACTG
  ACCCCTTCGCTCCCGTCTTTGTGCCACCACACCCCGGGATATCTGGGGGTCTTGGAGGAGCCTTGTCTGG
  GGCCTCTCGCTCCCTATCACCCACCCGCCTGCTCTCGCTGCCCCCGGACAAGCCGTTCGGCGCCAAGCCT
  CTAGGGTTCTGGACCAAGTTTGACGTCGCCGATTGGCTAGAGTGGCTGGGCTTGTCGGAGCACCGAGCCC
  AGTTCCTGGACCACGAGATTGATGGCTCCCATCTGCCTGCCTTGACCAAGGAGGACTATGTGGACCTGGG
  TGTAACCAGGGTGGGCCACCGTATGAACATTGACCGGGCTCTCAAATTTTTCCTGGAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210818 representing NM_031751
  Red=Cloning site Green=Tags(s)
  
  MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPQGTRGRGSGAPGNLASTRGLQGRSMSVPDDAHF
  SMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQVLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLRE
  YPQSFEKGVPYLEFRYKTRVYKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDS
  DSGETPLTLAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYKDRRG
  LTPLFHTAMVGGDPRCCELLLYNRAQLGIADENGWQEIHQACQRGHSQHLEHLLFYGAEPGAQNASGNTA
  LHICALYNKETCARILLYRGANKDVKNNNGQTPFQVAVIAGNFELGELIRNHREQDVVPFQESPKYAARR
  RGPPGAGLTVPPALLRANSDTSMALPDWMVFSAPGASSSGTPGPTSGPQGQSQPSAPSTKLSSGTLRSAS
  SPRGARARSPSRGRHPEDAKRQPRGRPSSSGTPRDGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFM
  AVKSYQAQGEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQEGRQESRSDKAKRL
  FRHYTVGSYDSFDAPSLIDGIDSGSDYIIKEKTVLLQKKDSEGFGFVLRGAKAQTPIEEFTPTPAFPALQ
  YLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVNMIRQGGNTLMVKVVMVTRHPDMDEAVHKKA
  SQQAKRLPPPAISLRSKSMTSELEEMVSPWKKKIEYEQQPAAVPSMEKKRTVYQMALNKLDEILAAAQQT
  ISASESPGPGGLASLGKHRPKGFFATESSFDPHHRSQPSYDRPSFLPPGPGLMLRQKSIGAAEDDRPYLA
  PPAMKFSRSLSVPGSEDIPPPPTTSPPEPPYSTPPAPSSSGRLTPSPRGGPFNPSSGGPLPASSPSSFDG
  PSPPDTRGGGREKSLYHSAALPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGGSPDDPPPRLALG
  PQPSLRGWRGGGPSPTSGAPSPSHHSSSGGSSGPTQAPALRYFQLPPRAASAAMYVPARSGRGRKGPLVK
  QTKVEGEPQKGSIPSASSPTSPALPRSEPPPAGPSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPT
  QPDGAGGGGSSPSPAPATSPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTL
  FLSTDAGDEDGGDSGLGPGGPPGPRLRHSKSIDEGMFSAEPYLRLESGGSSGGYGAYAAGSRAYGGSGSS
  SAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGTPQPPPRPPSPRYDAPPPTLHHHS
  PHSPHSPHARHEPVLRLWGDPARRELGYRAGLGSQEKALTASPPAARRSLLHRLPPTAPGVGPLLLQLGP
  EPPTPHPGVSKAWRTAAPEEPERLPLHVRFLENCQARPPPAGTRGSSTEDGPGVPPPSPRRVLPTSPTSP
  RGNEENGLPLLVLPPPAPSVDVDDGEFLFAEPLPPPLEFSNSFEKPESPLTPGPPHPLPDPPSPATPLPA
  APPPAVAAAPPTLDSTASSLTSYDSEVATLTQGAPAAPGDPPAPGPPAPAAPAPPAPQPGPDPPPGTDSG
  IEEVDSRSSSDHPLETISSASTLSSLSAEGGGNTGGVAGGGAGVASGTELLDTYVAYLDGQAFGGSGTPG
  PPYPPQLMTPSKLRGRALGTSGNLRPGPSGGLRDPVTPTSPTVSVTGAGTDGLLALSACPGPSTAGVAGG
  PVAVEPEVPPVPLPAASSLPRKLLPWEEGPGPPPPPLPGPLSQPQASALATVKASIISELSSKLQQFGGS
  STAGGALPWARGGSGGSTDSHHGGASYIPERTSSLQRQRLSEDSQTSLLSKPSSSIFQNWPKPPLPPLPT
  GSGVSSSTAAAPGATSPSASSASASTRHLQGVEFEMRPPLLRRAPSPSLLPASDHKVSPAPRPSSLPILP
  SGPIYPGLFDIRSSPTGGAGGSTDPFAPVFVPPHPGISGGLGGALSGASRSLSPTRLLSLPPDKPFGAKP
  LGFWTKFDVADWLEWLGLSEHRAQFLDHEIDGSHLPALTKEDYVDLGVTRVGHRMNIDRALKFFLER
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031751
• ORF Size: 6501 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031751.3
• RefSeq Size: 7585 bp
• RefSeq ORF: 6504 bp
• Locus ID: 78957
• UniProt ID: Q9WV48
• Cytogenetics: 1q22
• MW: 226.3 kDa
• Gene Summary: a scaffold protein in the post synaptic density that forms a complex with GKAP and PSD-95 [RGD, Feb 2006]