Pafah1b1 (NM_031763) Rat Tagged ORF Clone

CAT#: RR210778

Pafah1b1 (Myc-DDK-tagged ORF) - Rat platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa (Pafah1b1), (10 ug)

Product Images

Circular map for RR210778

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pafah1b1
• Synonyms: LIS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210778 representing NM_031763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGTCCCAGAGACAACGAGATGAACTAAATCGAGCTATAGCAGATTATCTTCGTTCAAATGGCT
  ATGAAGAGGCATATTCCGTTTTTAAAAAGGAAGCTGAACTAGATATGAATGAAGAATTAGATAAGAAGTA
  TGCTGGTCTTTTGGAAAAAAAATGGACATCTGTTATTAGATTACAAAAAAAGGTAATGGAATTAGAATCA
  AAACTAAATGAAGCAAAAGAAGAATTTACGTCAGGTGGTCCTCTTGGTCAGAAACGAGACCCAAAAGAAT
  GGATTCCCCGTCCACCTGAGAAATATGCATTGAGTGGTCATAGGAGTCCAGTTACTCGAGTCATTTTCCA
  TCCTGTGTTCAGTGTTATGGTCTCTGCTTCAGAGGATGCTACAATTAAGGTGTGGGATTATGAGACTGGA
  GATTTTGAGCGAACTCTCAAGGGGCATACAGATTCTGTACAGGACATTTCCTTTGACCACAGTGGCAAAC
  TCTTGGCTTCCTGTTCTGCAGATATGACGATTAAATTATGGGATTTTCAGGGTTTTGAATGCATCAGAAC
  CATGCACGGCCACGACCACAATGTCTCTTCAGTAGCCATCATGCCTAATGGAGATCATATAGTGTCTGCC
  TCAAGGGATAAAACTATAAAAATGTGGGAAGTGCAAACTGGCTACTGTGTGAAGACATTCACAGGACACA
  GAGAATGGGTACGTATGGTGCGGCCAAATCAGGACGGCACTCTGATAGCCAGCTGTTCCAATGACCAGAC
  TGTGCGTGTATGGGTTGTAGCAACAAAGGAATGCAAAGCTGAGCTCCGAGAACACGAACATGTGGTAGAA
  TGCATTTCCTGGGCTCCAGAAAGTTCATATTCTTCCATCTCTGAAGCAACAGGATCTGAGACTAAAAAAA
  GTGGCAAGCCTGGACCCTTCTTGCTATCTGGTTCCAGAGACAAAACTATTAAGATGTGGGACGTCAGTAC
  TGGCATGTGCCTTATGACTCTGGTGGGTCATGATAATTGGGTACGTGGAGTTCTGTTCCATTCTGGGGGG
  AAGTTTATTTTGAGTTGTGCTGATGACAAGACCCTCCGTGTATGGGATTACAAGAACAAGCGATGCATGA
  AGACCCTCAATGCGCATGAACACTTTGTTACCTCCTTGGATTTCCATAAGACGGCACCCTATGTGGTTAC
  TGGCAGTGTAGATCAAACAGTAAAGGTGTGGGAGTGCCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210778 representing NM_031763
  Red=Cloning site Green=Tags(s)
  
  MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELES
  KLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETG
  DFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSA
  SRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVE
  CISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGG
  KFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031763
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031763.3, NP_113951.1
• RefSeq Size: 4388 bp
• RefSeq ORF: 1233 bp
• Locus ID: 83572
• UniProt ID: P63004
• Cytogenetics: 10q24
• MW: 46.7 kDa
• Gene Summary: subunit of the brain intracellular platelet-activating factor acetylhydrolase, which is composed of alpha1, alpha2, and beta subunits [RGD, Feb 2006]