Pafah1b1 (NM_031763) Rat Untagged Clone

CAT#: RN210778

Pafah1b1 (untagged ORF) - Rat platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa (Pafah1b1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pafah1b1
• Synonyms: LIS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210778 representing NM_031763
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGTCCCAGAGACAACGAGATGAACTAAATCGAGCTATAGCAGATTATCTTCGTTCAAATGGCT
  ATGAAGAGGCATATTCCGTTTTTAAAAAGGAAGCTGAACTAGATATGAATGAAGAATTAGATAAGAAGTA
  TGCTGGTCTTTTGGAAAAAAAATGGACATCTGTTATTAGATTACAAAAAAAGGTAATGGAATTAGAATCA
  AAACTAAATGAAGCAAAAGAAGAATTTACGTCAGGTGGTCCTCTTGGTCAGAAACGAGACCCAAAAGAAT
  GGATTCCCCGTCCACCTGAGAAATATGCATTGAGTGGTCATAGGAGTCCAGTTACTCGAGTCATTTTCCA
  TCCTGTGTTCAGTGTTATGGTCTCTGCTTCAGAGGATGCTACAATTAAGGTGTGGGATTATGAGACTGGA
  GATTTTGAGCGAACTCTCAAGGGGCATACAGATTCTGTACAGGACATTTCCTTTGACCACAGTGGCAAAC
  TCTTGGCTTCCTGTTCTGCAGATATGACGATTAAATTATGGGATTTTCAGGGTTTTGAATGCATCAGAAC
  CATGCACGGCCACGACCACAATGTCTCTTCAGTAGCCATCATGCCTAATGGAGATCATATAGTGTCTGCC
  TCAAGGGATAAAACTATAAAAATGTGGGAAGTGCAAACTGGCTACTGTGTGAAGACATTCACAGGACACA
  GAGAATGGGTACGTATGGTGCGGCCAAATCAGGACGGCACTCTGATAGCCAGCTGTTCCAATGACCAGAC
  TGTGCGTGTATGGGTTGTAGCAACAAAGGAATGCAAAGCTGAGCTCCGAGAACACGAACATGTGGTAGAA
  TGCATTTCCTGGGCTCCAGAAAGTTCATATTCTTCCATCTCTGAAGCAACAGGATCTGAGACTAAAAAAA
  GTGGCAAGCCTGGACCCTTCTTGCTATCTGGTTCCAGAGACAAAACTATTAAGATGTGGGACGTCAGTAC
  TGGCATGTGCCTTATGACTCTGGTGGGTCATGATAATTGGGTACGTGGAGTTCTGTTCCATTCTGGGGGG
  AAGTTTATTTTGAGTTGTGCTGATGACAAGACCCTCCGTGTATGGGATTACAAGAACAAGCGATGCATGA
  AGACCCTCAATGCGCATGAACACTTTGTTACCTCCTTGGATTTCCATAAGACGGCACCCTATGTGGTTAC
  TGGCAGTGTAGATCAAACAGTAAAGGTGTGGGAGTGCCGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031763
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031763.3, NP_113951.1
• RefSeq Size: 4388 bp
• RefSeq ORF: 1233 bp
• Locus ID: 83572
• UniProt ID: P63004
• Cytogenetics: 10q24
• Gene Summary: subunit of the brain intracellular platelet-activating factor acetylhydrolase, which is composed of alpha1, alpha2, and beta subunits [RGD, Feb 2006]