Jak1 (NM_053466) Rat Tagged ORF Clone

CAT#: RR209166

Jak1 (Myc-DDK-tagged ORF) - Rat Janus kinase 1 (Jak1), (10 ug)

Product Images

Circular map for RR209166

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jak1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209166 representing NM_053466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTATCTAAATATAAAAGAGGACTGCAATGCCATGGCTTTCTGTGCTAAAATGAGGAGCTTCAAGA
  AGACGGAGGTGAAGCAGGTGGCCCCTGAGCCAGGAGTGGAAGTGACTTTCTATCTGTTGGACAGGGAGCC
  CCTCCGCCTGGGCAGCGGAGAGTACACCGCGGAGGAGCTGTGCATCAGGGCCGCACAGGAATGCAGTATC
  TCTCCTCTCTGCCACAACCTCTTCGCCTTGTATGATGAGAGCACCAAGCTCTGGTATGCTCCAAACCACA
  TCATCACTGTGGATGACAAAATGTCTCTCCGGCTGCACTACCGCATGAGGTTCTACTTTACCAACTGGCA
  TGGAACCAATGACAATGAACAGTCTGTATGGCGACATTCTCCAAAGAAGCAGAAAAATGGCTATGAGAAG
  AAAAAGGTTCCAGAAGCAACCCCACTCCTTGATGCCAGTTCACTGGAGTATCTGTTTGCGCAGGGACAGT
  ATGATTTGATCAAATGCCTGGCTCCCATTCGGGACCCCAAGACAGAGCAAGATGGACATGATATTGAGAA
  TGAGTGCCTGGGCATGGCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCCAGAG
  CTTCCCAAAGACATCAGCTACAAGCGATATATTCCAGAAACATTGAATAAATCCATCAGACAGAGGAATC
  TTCTTACCAGGATGCGAATAAATAATGTTTTCAAGGATTTCTTGAAGGAATTTAACAACAAGACCATCTG
  TGACAGCAGTGTGTCTACACACGACCTGAAGGTGAAGTACCTGGCTACCTTGGAAACCTTAACCAAGCAT
  TATGGAGCCGAGATATTTGAGACTTCTATGCTCCTGATTTCATCAGAAAATGAGCTGAGTCGGTGCCATT
  CCAACGACAGTGGCAATGTTCTCTATGAAGTCATGGTAACAGGAAATCTCGGGATCCAGTGGCGGCAGAA
  ACCAAATGTTCTTCCTGTTGAAAAGGAAAAAAATAAACTGAAGCGGAAAAAACTGGACTATAATAAACAC
  AAGAAGGAGGATGACAGAAACAAACTCCGGGAAGAGTGGAACAGTTTTTCCTATTTCCCCGAGATTACCC
  ACATTGTTATCAAGGAGTCTGTGGTCAGCATTAACAAACAGGACAACAAGAACATGGAACTAAAGCTCTC
  TTCTCATGAGGAAGCCTTGTCCTTCGTGTCCCTGGTAGATGGCTACTTCCGGCTCACTGCAGATGCTCAC
  CATTACCTCTGTACTGATGTGGCTCCCCCGCTGATTGTCCACAACATACAGAATGGCTGTCACGGTCCAA
  TCTGCACAGAATATGCCATCAATAAGCTGCGGCAGGAGGGGAGTGAGGAGGGGATGTACGTGCTGAGGTG
  GAGCTGCACCGACTTTGACAACATTCTTATGACTGTCAGCTGCTTCGAAAAGTCCGAGGTATTGGGTAAT
  CAGAAGCAGTTCAAGAACTTTCAGATTGAGGTGCAGAAGGGCCGCTACAGCCTGCATGGCTCTGTTGACC
  ACTTTCCCAGCCTGAGAGACCTCATGAACCACCTCAAGAAGCAGATCCTGCGCACGGACAATATAAGCTT
  TGTGCTGAAACGCTGCTGTCAGCCTAAGCCTCGAGAAATCTCCAACCTACTTGTAGCCACTAAGAAAGCC
  CAGGAGTGGCAGCCTGTCTACTCTATGAGCCAGCTGAGTTTCGATCGGATCCTTAAGAAAGATATTATCC
  AAGGTGAGCACCTTGGCAGAGGCACAAGAACACATATCTATTCTGGGACCCTGCTGGATTACAAGGACGA
  TGAAGGAATTGCTGAAGAAAAGAAGATAAAAGTGATTCTCAAAGTCCTAGACCCTAGCCACCGGGACATT
  TCACTGGCCTTCTTTGAGGCAGCCAGCATGATGAGACAGGTTTCCCACAAACACATCGTGTACCTCTACG
  GCGTCTGTGTCCGAGATGTGGAAAATATCATGGTGGAAGAGTTTGTGGAGGGGGGGCCATTGGATCTCTT
  CATGCACCGGAAAAGTGATGCACTTACTACCCCTTGGAAGTTTAAAGTTGCCAAACAGCTGGCCAGCGCC
  CTGAGTTACTTGGAAGATAAAGACCTCGTTCATGGAAATGTGTGCACTAAAAACCTCCTCCTGGCCCGTG
  AGGGCATTGACAGTGACATTGGCCCGTTCATCAAGCTTAGTGACCCTGGCATCCCAGTCTCTGTGCTGAC
  CAGGCAAGAGTGCATAGAGCGAATTCCCTGGATCGCTCCTGAGTGTGTTGAAGACTCCAAGAACCTGAGT
  GTGGCTGCTGACAAGTGGAGCTTTGGAACCACACTCTGGGAAATCTGCTACAATGGCGAGATCCCACTCA
  AGGACAAGACCCTCATTGAGAAAGAGAGGTTTTATGAAAGCCGCTGCAGGCCAGTGACACCATCTTGCAA
  GGAGCTAGCTGACCTCATGACTCGCTGCATGAACTATGATCCCAACCAGAGACCTTTCTTCCGAGCCATC
  ATGAGGGACATTAACAAGCTGGAGGAGCAGAATCCAGACATTGTTTCAGAAAAGCAGCCAATAACAGAGG
  TGGATCCCACTCATTTTGAAAAGCGTTTCCTAAAGAGGATTCGTGACTTGGGAGAGGGTCACTTTGGGAA
  GGTTGAGCTCTGCAGATATGATCCTGAGGGAGACAACACAGGGGAACAGGTAGCTGTCAAGTCCCTGAAG
  CCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAGAAGGAGATAGAGATCTTACGGAACCTCTACCACG
  AGAACATTGTGAAGTATAAAGGAATCTGCATGGAAGACGGAGGGAATGGTATCAAGCTCATCATGGAGTT
  TCTGCCTTCGGGAAGCCTAAAGGAATATCTGCCAAAGAATAAGAACAAAATCAACCTCAAACAGCAGCTA
  AAATATGCCATCCAGATTTGTAAGGGGATGGACTATCTGGGCTCTCGGCAATATGTTCACCGGGACTTAG
  CAGCAAGAAATGTCCTTGTTGAGAGTGAACACCAAGTGAAGATCGGAGACTTTGGTTTAACCAAAGCAAT
  TGAGACCGATAAGGAGTACTACACAGTCAAGGATGACCGGGACAGCCCAGTGTTTTGGTACGCTCCGGAG
  TGTTTAATCCAGTGTAAATTTTATATCGCCTCTGATGTCTGGTCTTTTGGAGTGACACTGCACGAGTTGC
  TCACGTACTGTGACTCAGATTTTAGTCCCATGGCCTTGTTCCTGAAAATGATAGGCCCAACTCATGGCCA
  GATGACAGTAACACGGCTTGTGAATACTCTGAAAGAAGGAAAGCGCCTGTCCTGTCCACCCAACTGTCCC
  GATGAGGTTTATCAGCTTATGAGAAAATGCTGGGAATTCCAACCATCTAATCGGACAACCTTTCAGAACC
  TTATTGAAGGATTTGAAGCACTTTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209166 representing NM_053466
  Red=Cloning site Green=Tags(s)
  
  MQYLNIKEDCNAMAFCAKMRSFKKTEVKQVAPEPGVEVTFYLLDREPLRLGSGEYTAEELCIRAAQECSI
  SPLCHNLFALYDESTKLWYAPNHIITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEK
  KKVPEATPLLDASSLEYLFAQGQYDLIKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPE
  LPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKH
  YGAEIFETSMLLISSENELSRCHSNDSGNVLYEVMVTGNLGIQWRQKPNVLPVEKEKNKLKRKKLDYNKH
  KKEDDRNKLREEWNSFSYFPEITHIVIKESVVSINKQDNKNMELKLSSHEEALSFVSLVDGYFRLTADAH
  HYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVSCFEKSEVLGN
  QKQFKNFQIEVQKGRYSLHGSVDHFPSLRDLMNHLKKQILRTDNISFVLKRCCQPKPREISNLLVATKKA
  QEWQPVYSMSQLSFDRILKKDIIQGEHLGRGTRTHIYSGTLLDYKDDEGIAEEKKIKVILKVLDPSHRDI
  SLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDALTTPWKFKVAKQLASA
  LSYLEDKDLVHGNVCTKNLLLAREGIDSDIGPFIKLSDPGIPVSVLTRQECIERIPWIAPECVEDSKNLS
  VAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAI
  MRDINKLEEQNPDIVSEKQPITEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLK
  PESGGNHIADLKKEIEILRNLYHENIVKYKGICMEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQL
  KYAIQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPE
  CLIQCKFYIASDVWSFGVTLHELLTYCDSDFSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLSCPPNCP
  DEVYQLMRKCWEFQPSNRTTFQNLIEGFEALLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053466
• ORF Size: 3459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053466.1, NP_445918.1
• RefSeq Size: 4291 bp
• RefSeq ORF: 3462 bp
• Locus ID: 84598
• Cytogenetics: 5q33
• MW: 133.3 kDa
• Gene Summary: protein-tyrosine kinases; involved in intracellular signal transduction [RGD, Feb 2006]