Tpm4 (NM_012678) Rat Tagged ORF Clone
CAT#: RR204596
- TrueORF®
Tpm4 (Myc-DDK-tagged ORF) - Rat tropomyosin 4 (Tpm4), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_012678" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tpm4 |
Synonyms | Tpm4.2; Tpm4.2cy |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204596 representing NM_012678
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCCTCAACTCACTGGAGGCGGTGAAGCGCAAGATCCAGGCCCTGCAGCAGCAGGCGGACGACG CAGAGGACCGTGCGCAGGGCCTGCAGCGCGAGCTGGATGGCGAGCGCGAACGGCGCGAGAAAGCTGAAGG AGATGCGGCCGCTCTCAACCGTCGCATCCAGCTGGTGGAGGAAGAGCTGGACCGGGCTCAGGAGCGACTG GCCACAGCCCTGCAGAAGCTGGAGGAGGCAGAGAAGGCTGCTGACGAGAGTGAGAGAGGCATGAAGGTGA TAGAGAACCGAGCCATGAAAGACGAGGAGAAGATGGAGATCCAGGAGATGCAGCTCAAAGAAGCCAAGCA CATCGCTGAGGAGGCTGACCGGAAGTATGAGGAGGTTGCTCGTAAGTTGGTCATCCTGGAGGGTGAGCTG GAGAGAGCAGAGGAGCGGGCGGAGGTGTCTGAACTAAAGAGTAGCGACCTGGAAGAGGAGCTCAAGAACG TAACTAACAATCTGAAATCACTGGAGGCTGCTTCTGAAAAGTACTCTGAAAAGGAGGATAAATATGAAGA AGAAATCAAGCTTCTGTCTGACAAACTGAAAGAGGCTGAGACCCGAGCTGAGTTTGCGGAAAGGACAGTT TCTAAACTGGAGAAGACAATCGATGACCTGGAAGAAAAACTTGCCCAGGCCAAAGAAGAGAACGTGGGCT TGCATCAGACACTGGACCAGACACTAAACGAACTTAACTGTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204596 representing NM_012678
Red=Cloning site Green=Tags(s) MAGLNSLEAVKRKIQALQQQADDAEDRAQGLQRELDGERERREKAEGDAAALNRRIQLVEEELDRAQERL ATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGEL ERAEERAEVSELKSSDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTV SKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012678 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012678.2, NP_036810.1 |
RefSeq Size | 895 bp |
RefSeq ORF | 747 bp |
Locus ID | 24852 |
UniProt ID | P09495 |
Cytogenetics | 16p14 |
MW | 28.5 kDa |
Gene Summary | binds actin filaments; important for calcium-dependent regulation of striated muscle contraction; may be important for stabilizing actin filaments in the cytoskeleton [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204596 | Tpm4 (untagged ORF) - Rat tropomyosin 4 (Tpm4), (10 ug) |
USD 330.00 |
|
RR204596L3 | Lenti ORF clone of Tpm4 (Myc-DDK-tagged ORF) - Rat tropomyosin 4 (Tpm4), (10 ug) |
USD 630.00 |
|
RR204596L4 | Lenti ORF clone of Tpm4 (mGFP-tagged ORF) - Rat tropomyosin 4 (Tpm4), (10 ug) |
USD 630.00 |
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