Tpm4 (NM_012678) Rat Untagged Clone

CAT#: RN204596

Tpm4 (untagged ORF) - Rat tropomyosin 4 (Tpm4), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tpm4
• Synonyms: Tpm4.2; Tpm4.2cy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204596 representing NM_012678
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCTCAACTCACTGGAGGCGGTGAAGCGCAAGATCCAGGCCCTGCAGCAGCAGGCGGACGACG
  CAGAGGACCGTGCGCAGGGCCTGCAGCGCGAGCTGGATGGCGAGCGCGAACGGCGCGAGAAAGCTGAAGG
  AGATGCGGCCGCTCTCAACCGTCGCATCCAGCTGGTGGAGGAAGAGCTGGACCGGGCTCAGGAGCGACTG
  GCCACAGCCCTGCAGAAGCTGGAGGAGGCAGAGAAGGCTGCTGACGAGAGTGAGAGAGGCATGAAGGTGA
  TAGAGAACCGAGCCATGAAAGACGAGGAGAAGATGGAGATCCAGGAGATGCAGCTCAAAGAAGCCAAGCA
  CATCGCTGAGGAGGCTGACCGGAAGTATGAGGAGGTTGCTCGTAAGTTGGTCATCCTGGAGGGTGAGCTG
  GAGAGAGCAGAGGAGCGGGCGGAGGTGTCTGAACTAAAGAGTAGCGACCTGGAAGAGGAGCTCAAGAACG
  TAACTAACAATCTGAAATCACTGGAGGCTGCTTCTGAAAAGTACTCTGAAAAGGAGGATAAATATGAAGA
  AGAAATCAAGCTTCTGTCTGACAAACTGAAAGAGGCTGAGACCCGAGCTGAGTTTGCGGAAAGGACAGTT
  TCTAAACTGGAGAAGACAATCGATGACCTGGAAGAAAAACTTGCCCAGGCCAAAGAAGAGAACGTGGGCT
  TGCATCAGACACTGGACCAGACACTAAACGAACTTAACTGTATATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012678
• Insert Size: 747 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012678.2, NP_036810.1
• RefSeq Size: 895 bp
• RefSeq ORF: 747 bp
• Locus ID: 24852
• UniProt ID: P09495
• Cytogenetics: 16p14
• Gene Summary: binds actin filaments; important for calcium-dependent regulation of striated muscle contraction; may be important for stabilizing actin filaments in the cytoskeleton [RGD, Feb 2006]