Pklr (NM_012624) Rat Tagged ORF Clone

CAT#: RR203014

Pklr (Myc-DDK-tagged ORF) - Rat pyruvate kinase, liver and RBC (Pklr), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR203014

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pklr
• Synonyms: PK1; PKL; Pklg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203014 representing NM_012624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCAGCGGGATACCTTCGACGTGCGAGTGTGGCTCAACTGACCCAGGAGCTGGGCACTGCCT
  TCTTCCAGCAGCAGCAACTGCCCGCAGCTATGGCGGACACCTTCCTGGAACACCTCTGCCTTCTGGATAT
  CGACTCACAGCCTGTGGCTGCTCGTAGCACCAGCATCATTGCCACCATTGGGCCAGCATCCCGCTCTGTG
  GACCGCCTCAAGGAGATGATCAAAGCAGGGATGAACATTGCACGACTCAACTTCTCCCATGGCTCCCATG
  AGTACCATGCAGAATCCATCGCCAACATCCGGGAGGCAACTGAGAGTTTTGCAACCTCCCCACTCAGCTA
  CAGACCTGTGGCCATCGCCCTGGACACCAAGGGACCTGAGATACGAACCGGAGTCTTGCAGGGGGGTCCG
  GAGTCGGAGGTGGAAATTGTGAAGGGCTCACAGGTGCTGGTGACGGTGGACCCGAAGTTCCAGACAAGGG
  GCGATGCAAAGACAGTGTGGGTGGACTACCACAATATCACCCGGGTCGTTGCAGTGGGGGGCCGCATCTA
  CATTGACGACGGGCTCATCTCCTTAGTGGTACAGAAAATCGGCCCAGAGGGACTGGTGACAGAAGTGGAG
  CACGGTGGTATCTTGGGCAGCAGGAAGGGTGTGAACTTGCCAAACACTGAGGTGGACCTGCCCGGGCTGT
  CTGAGCAAGACCTTTTGGATCTGCGCTTCGGGGTGCAGCATAATGTGGACATCATCTTTGCCTCCTTTGT
  GCGGAAAGCCAGTGACGTGTTAGCAGTCCGGGATGCCCTGGGGCCAGAAGGACAGAACATCAAAATTATC
  AGCAAAATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAAATTCTAGAAGTGAGCGATGGCATCATGG
  TGGCACGGGGTGACCTGGGCATTGAGATCCCTGCGGAGAAGGTTTTCTTGGCTCAGAAGATGATGATTGG
  ACGCTGCAACCTGGCCGGCAAGCCTGTCGTTTGTGCCACACAGATGCTGGAGAGCATGATCACTAAGGCT
  CGACCAACTCGGGCGGAGACAAGCGATGTGGCCAATGCCGTGCTGGATGGGGCTGACTGTATCATGCTGT
  CCGGAGAGACCGCCAAGGGCAGTTTTCCTGTGGAAGCTGTAATGATGCAACATGCGATTGCGCGGGAGGC
  AGAGGCCGCTGTGTACCACCGCCAGTTGTTTGAGGAGCTACGCCGGGCAGCGCCGCTGAGCCGTGACCCA
  ACTGAGGTCACTGCGATTGGAGCCGTGGAGGCTTCCTTCAAGTGCTGTGCAGCAGCCATCATCGTGCTGA
  CGAAGACTGGCCGTTCAGCCCAGCTTCTATCTCAATACCGACCTCGGGCGGCTGTCATTGCTGTGACTCG
  ATCTGCCCAGGCTGCCCGACAGGTCCACCTGTCCCGAGGAGTCTTCCCCTTGCTCTACCGTGAGCCTCCA
  GAGGCCATCTGGGCAGATGATGTGGATCGAAGGGTCCAATTTGGCATTGAAAGTGGAAAGCTCCGTGGTT
  TCCTCCGTGTGGGTGATCTGGTGATTGTGGTGACAGGTTGGCGGCCTGGCTCTGGCTATACCAACATCAT
  GCGGGTGCTGAGCGTATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203014 representing NM_012624
  Red=Cloning site Green=Tags(s)
  
  MEGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSV
  DRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGP
  ESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVE
  HGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKII
  SKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKA
  RPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDP
  TEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPP
  EAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012624
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012624.3, NP_036756.3
• RefSeq Size: 2922 bp
• RefSeq ORF: 1632 bp
• Locus ID: 24651
• UniProt ID: P12928
• Cytogenetics: 2q34
• MW: 58.8 kDa
• Gene Summary: L-isozyme of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006]