Pklr (NM_012624) Rat Untagged Clone

CAT#: RN203014

Pklr (untagged ORF) - Rat pyruvate kinase, liver and RBC (Pklr), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pklr
• Synonyms: PK1; PKL; Pklg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203014 representing NM_012624
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCAGCGGGATACCTTCGACGTGCGAGTGTGGCTCAACTGACCCAGGAGCTGGGCACTGCCT
  TCTTCCAGCAGCAGCAACTGCCCGCAGCTATGGCGGACACCTTCCTGGAACACCTCTGCCTTCTGGATAT
  CGACTCACAGCCTGTGGCTGCTCGTAGCACCAGCATCATTGCCACCATTGGGCCAGCATCCCGCTCTGTG
  GACCGCCTCAAGGAGATGATCAAAGCAGGGATGAACATTGCACGACTCAACTTCTCCCATGGCTCCCATG
  AGTACCATGCAGAATCCATCGCCAACATCCGGGAGGCAACTGAGAGTTTTGCAACCTCCCCACTCAGCTA
  CAGACCTGTGGCCATCGCCCTGGACACCAAGGGACCTGAGATACGAACCGGAGTCTTGCAGGGGGGTCCG
  GAGTCGGAGGTGGAAATTGTGAAGGGCTCACAGGTGCTGGTGACGGTGGACCCGAAGTTCCAGACAAGGG
  GCGATGCAAAGACAGTGTGGGTGGACTACCACAATATCACCCGGGTCGTTGCAGTGGGGGGCCGCATCTA
  CATTGACGACGGGCTCATCTCCTTAGTGGTACAGAAAATCGGCCCAGAGGGACTGGTGACAGAAGTGGAG
  CACGGTGGTATCTTGGGCAGCAGGAAGGGTGTGAACTTGCCAAACACTGAGGTGGACCTGCCCGGGCTGT
  CTGAGCAAGACCTTTTGGATCTGCGCTTCGGGGTGCAGCATAATGTGGACATCATCTTTGCCTCCTTTGT
  GCGGAAAGCCAGTGACGTGTTAGCAGTCCGGGATGCCCTGGGGCCAGAAGGACAGAACATCAAAATTATC
  AGCAAAATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAAATTCTAGAAGTGAGCGATGGCATCATGG
  TGGCACGGGGTGACCTGGGCATTGAGATCCCTGCGGAGAAGGTTTTCTTGGCTCAGAAGATGATGATTGG
  ACGCTGCAACCTGGCCGGCAAGCCTGTCGTTTGTGCCACACAGATGCTGGAGAGCATGATCACTAAGGCT
  CGACCAACTCGGGCGGAGACAAGCGATGTGGCCAATGCCGTGCTGGATGGGGCTGACTGTATCATGCTGT
  CCGGAGAGACCGCCAAGGGCAGTTTTCCTGTGGAAGCTGTAATGATGCAACATGCGATTGCGCGGGAGGC
  AGAGGCCGCTGTGTACCACCGCCAGTTGTTTGAGGAGCTACGCCGGGCAGCGCCGCTGAGCCGTGACCCA
  ACTGAGGTCACTGCGATTGGAGCCGTGGAGGCTTCCTTCAAGTGCTGTGCAGCAGCCATCATCGTGCTGA
  CGAAGACTGGCCGTTCAGCCCAGCTTCTATCTCAATACCGACCTCGGGCGGCTGTCATTGCTGTGACTCG
  ATCTGCCCAGGCTGCCCGACAGGTCCACCTGTCCCGAGGAGTCTTCCCCTTGCTCTACCGTGAGCCTCCA
  GAGGCCATCTGGGCAGATGATGTGGATCGAAGGGTCCAATTTGGCATTGAAAGTGGAAAGCTCCGTGGTT
  TCCTCCGTGTGGGTGATCTGGTGATTGTGGTGACAGGTTGGCGGCCTGGCTCTGGCTATACCAACATCAT
  GCGGGTGCTGAGCGTATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012624
• Insert Size: 1632 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012624.3, NP_036756.3
• RefSeq Size: 2922 bp
• RefSeq ORF: 1632 bp
• Locus ID: 24651
• UniProt ID: P12928
• Cytogenetics: 2q34
• Gene Summary: L-isozyme of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006]