Hspa5 (NM_013083) Rat Tagged ORF Clone

CAT#: RR201826

Hspa5 (Myc-DDK-tagged ORF) - Rat heat shock protein 5 (Hspa5), (10 ug)

Product Images

Circular map for RR201826

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa5
• Synonyms: BIP; GRP 78; GRP78
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201826 representing NM_013083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCACTGTGGTGGCGGCGGCGCTGCTGCTGCTGTGTGCGGTGCGGGCGGAGGAGGAGGACAAGA
  AGGAGGATGTAGGCACGGTGGTTGGCATCGACTTGGGGACCACCTATTCCTGCGTCGGTGTATTCAAGAA
  CGGCCGCGTGGAGATCATAGCCAACGATCAGGGCAACCGCATCACACCGTCGTATGTGGCCTTCACTCCT
  GAAGGGGAGCGTCTGATTGGCGATGCGGCCAAGAACCAACTCACGTCCAACCCGGAGAACACCGTCTTCG
  ACGCCAAGCGCCTCATCGGACGCACTTGGAATGACCCTTCCGTGCAGCAGGACATCAAGTTCTTGCCATT
  CAAGGTGGTTGAAAAGAAAACTAAACCATATATTCAAGTTGATATCGGAGGTGGGCAAACCAAGACATTT
  GCCCCAGAAGAAATTTCTGCTATGGTTCTCACTAAAATGAAGGAAACTGCCGAGGCGTATTTGGGAAAGA
  AGGTCACCCATGCAGTTGTGACTGTACCAGCTTACTTCAATGATGCACAGCGGCAAGCAACCAAGGATGC
  TGGCACTATTGCTGGACTGAATGTCATGAGGATCATCAATGAGCCAACAGCAGCTGCTATTGCATATGGC
  CTGGATAAGAGAGAGGGAGAGAAGAACATCCTCGTGTTTGACCTGGGTGGTGGAACCTTCGATGTGTCTC
  TTCTCACCATTGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGACACTCATCTGGGTGGGGAAGA
  CTTTGATCAGCGGGTCATGGAGCACTTCATCAAGCTGTACAAAAAGAAGACTGGGAAAGATGTCAGAAAG
  GACAACAGAGCTGTGCAGAAACTCCGGCGTGAGGTAGAAAAGGCCAAGAGAGCCCTGTCTTCTCAGCATC
  AAGCGAGGATTGAAATTGAGTCCTTCTTCGAAGGAGAAGACTTCTCAGAGACCCTTACTCGGGCCAAATT
  TGAAGAGCTGAACATGGACCTGTTCCGCTCTACCATGAAACCTGTCCAGAAAGTGTTGGAAGACTCTGAT
  CTGAAGAAATCTGATATTGATGAGATTGTTCTGGTTGGCGGATCTACTCGAATTCCAAAGATTCAGCAGT
  TGGTGAAAGAGTTCTTCAATGGCAAGGAGCCGTCCCGTGGCATCAACCCAGATGAGGCTGTAGCATATGG
  TGCCGCTGTCCAGGCTGGTGTCCTCTCTGGTGATCAAGATACAGGTGACCTGGTTCTGCTTGATGTGTGT
  CCTCTTACACTTGGTATTGAAACTGTGGGAGGTGTCATGACAAAACTGATTCCGAGGAACACTGTGGTGC
  CCACCAAGAAGTCTCAGATCTTCTCCACAGCTTCTGATAATCAGCCCACCGTAACAATCAAGGTCTACGA
  AGGTGAACGACCCCTGACAAAAGACAATCACCTTCTGGGTACATTTGATCTGACTGGAATCCCTCCTGCT
  CCCCGTGGGGTACCCCAGATTGAAGTCACCTTTGAGATAGATGTGAATGGTATTCTTCGAGTGACAGCTG
  AAGACAAAGGGACAGGAAACAAAAACAAAATCACCATCACCAATGACCAAAACCGCCTGACCCCTGAAGA
  AATTGAAAGGATGGTCAATGACGCCGAGAAGTTTGCTGAGGAAGACAAAAAGCTCAAAGAGCGCATTGAC
  ACGAGGAATGAATTGGAAAGCTATGCTTACTCTCTTAAGAACCAGATCGGAGATAAAGAGAAGCTGGGAG
  GTAAGCTGTCTCCTGAAGATAAAGAGACCATGGAGAAAGCTGTAGAGGAAAAGATCGAATGGCTGGAAAG
  CCACCAGGATGCAGACATTGAAGACTTTAAAGCTAAAAAGAAGGAACTAGAAGAAATTGTTCAGCCAATT
  ATCAGCAAACTCTATGGAAGTGGAGGCCCTCCCCCAACTGGTGAAGAGGATACATCAGAAAAAGATGAGT
  TG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201826 representing NM_013083
  Red=Cloning site Green=Tags(s)
  
  MKFTVVAAALLLLCAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTP
  EGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTF
  APEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYG
  LDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRK
  DNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSD
  LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVC
  PLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPA
  PRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERID
  TRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPI
  ISKLYGSGGPPPTGEEDTSEKDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013083
• ORF Size: 1962 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013083.2, NP_037215.1
• RefSeq Size: 2580 bp
• RefSeq ORF: 1965 bp
• Locus ID: 25617
• UniProt ID: P06761
• Cytogenetics: 3p11
• MW: 72.3 kDa
• Gene Summary: secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]