Hspa5 (NM_013083) Rat Untagged Clone

CAT#: RN201826

Hspa5 (untagged ORF) - Rat heat shock protein 5 (Hspa5), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hspa5
• Synonyms: BIP; GRP 78; GRP78
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201826 representing NM_013083
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCACTGTGGTGGCGGCGGCGCTGCTGCTGCTGTGTGCGGTGCGGGCGGAGGAGGAGGACAAGA
  AGGAGGATGTAGGCACGGTGGTTGGCATCGACTTGGGGACCACCTATTCCTGCGTCGGTGTATTCAAGAA
  CGGCCGCGTGGAGATCATAGCCAACGATCAGGGCAACCGCATCACACCGTCGTATGTGGCCTTCACTCCT
  GAAGGGGAGCGTCTGATTGGCGATGCGGCCAAGAACCAACTCACGTCCAACCCGGAGAACACCGTCTTCG
  ACGCCAAGCGCCTCATCGGACGCACTTGGAATGACCCTTCCGTGCAGCAGGACATCAAGTTCTTGCCATT
  CAAGGTGGTTGAAAAGAAAACTAAACCATATATTCAAGTTGATATCGGAGGTGGGCAAACCAAGACATTT
  GCCCCAGAAGAAATTTCTGCTATGGTTCTCACTAAAATGAAGGAAACTGCCGAGGCGTATTTGGGAAAGA
  AGGTCACCCATGCAGTTGTGACTGTACCAGCTTACTTCAATGATGCACAGCGGCAAGCAACCAAGGATGC
  TGGCACTATTGCTGGACTGAATGTCATGAGGATCATCAATGAGCCAACAGCAGCTGCTATTGCATATGGC
  CTGGATAAGAGAGAGGGAGAGAAGAACATCCTCGTGTTTGACCTGGGTGGTGGAACCTTCGATGTGTCTC
  TTCTCACCATTGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGACACTCATCTGGGTGGGGAAGA
  CTTTGATCAGCGGGTCATGGAGCACTTCATCAAGCTGTACAAAAAGAAGACTGGGAAAGATGTCAGAAAG
  GACAACAGAGCTGTGCAGAAACTCCGGCGTGAGGTAGAAAAGGCCAAGAGAGCCCTGTCTTCTCAGCATC
  AAGCGAGGATTGAAATTGAGTCCTTCTTCGAAGGAGAAGACTTCTCAGAGACCCTTACTCGGGCCAAATT
  TGAAGAGCTGAACATGGACCTGTTCCGCTCTACCATGAAACCTGTCCAGAAAGTGTTGGAAGACTCTGAT
  CTGAAGAAATCTGATATTGATGAGATTGTTCTGGTTGGCGGATCTACTCGAATTCCAAAGATTCAGCAGT
  TGGTGAAAGAGTTCTTCAATGGCAAGGAGCCGTCCCGTGGCATCAACCCAGATGAGGCTGTAGCATATGG
  TGCCGCTGTCCAGGCTGGTGTCCTCTCTGGTGATCAAGATACAGGTGACCTGGTTCTGCTTGATGTGTGT
  CCTCTTACACTTGGTATTGAAACTGTGGGAGGTGTCATGACAAAACTGATTCCGAGGAACACTGTGGTGC
  CCACCAAGAAGTCTCAGATCTTCTCCACAGCTTCTGATAATCAGCCCACCGTAACAATCAAGGTCTACGA
  AGGTGAACGACCCCTGACAAAAGACAATCACCTTCTGGGTACATTTGATCTGACTGGAATCCCTCCTGCT
  CCCCGTGGGGTACCCCAGATTGAAGTCACCTTTGAGATAGATGTGAATGGTATTCTTCGAGTGACAGCTG
  AAGACAAAGGGACAGGAAACAAAAACAAAATCACCATCACCAATGACCAAAACCGCCTGACCCCTGAAGA
  AATTGAAAGGATGGTCAATGACGCCGAGAAGTTTGCTGAGGAAGACAAAAAGCTCAAAGAGCGCATTGAC
  ACGAGGAATGAATTGGAAAGCTATGCTTACTCTCTTAAGAACCAGATCGGAGATAAAGAGAAGCTGGGAG
  GTAAGCTGTCTCCTGAAGATAAAGAGACCATGGAGAAAGCTGTAGAGGAAAAGATCGAATGGCTGGAAAG
  CCACCAGGATGCAGACATTGAAGACTTTAAAGCTAAAAAGAAGGAACTAGAAGAAATTGTTCAGCCAATT
  ATCAGCAAACTCTATGGAAGTGGAGGCCCTCCCCCAACTGGTGAAGAGGATACATCAGAAAAAGATGAGT
  TGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013083
• Insert Size: 1965 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013083.2, NP_037215.1
• RefSeq Size: 2580 bp
• RefSeq ORF: 1965 bp
• Locus ID: 25617
• UniProt ID: P06761
• Cytogenetics: 3p11
• Gene Summary: secreted protein of the endoplasmic reticulum; may be involved in the assembly of secreted and membrane-bound proteins [RGD, Feb 2006]