ADAM23 (NM_003812) Human Tagged ORF Clone

CAT#: RG222765

ADAM23 (tGFP-tagged) - Human ADAM metallopeptidase domain 23 (ADAM23)

Product Images

Circular map for RG222765

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADAM23
• Synonyms: MDC-3; MDC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222765 representing NM_003812
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCGCCCGGCAGCAGCTCGCGGCAGCCGCCCCTGGCGGGCTGCAGCCTTGCCGGCGCTTCCTGCG
  GCCCCCAACGCGGCCCCGCCGGCTCGGTGCCTGCCAGCGCCCCGGCCCGCACGCCGCCCTGCCGCCTGCT
  TCTCGTCCTTCTCCTGCTGCCTCCGCTCGCCGCCTCGTCCCGGCCCCGCGCCTGGGGGGCTGCTGCGCCC
  AGCGCTCCGCATTGGAATGAAACTGCAGAAAAAAATTTGGGAGTCCTGGCAGATGAAGACAATACATTGC
  AACAGAATAGCAGCAGTAATATCAGTTACAGCAATGCAATGCAGAAAGAAATCACACTGCCTTCAAGACT
  CATATATTACATCAACCAAGACTCGGAAAGCCCTTATCACGTTCTTGACACAAAGGCAAGACACCAGCAA
  AAACATAATAAGGCTGTCCATCTGGCCCAGGCAAGCTTCCAGATTGAAGCCTTCGGCTCCAAATTCATTC
  TTGACCTCATACTGAACAATGGTTTGTTGTCTTCTGATTATGTGGAGATTCACTACGAAAATGGGAAACC
  ACAGTACTCTAAGGGTGGAGAGCACTGTTACTACCATGGAAGCATCAGAGGCGTCAAAGACTCCAAGGTG
  GCTCTGTCAACCTGCAATGGACTTCATGGCATGTTTGAAGATGATACCTTCGTGTATATGATAGAGCCAC
  TAGAGCTGGTTCATGATGAGAAAAGCACAGGTCGACCACATATAATCCAGAAAACCTTGGCAGGACAGTA
  TTCTAAGCAAATGAAGAATCTCACTATGGAAAGAGGTGACCAGTGGCCCTTTCTCTCTGAATTACAGTGG
  TTGAAAAGAAGGAAGAGAGCAGTGAATCCATCACGTGGTATATTTGAAGAAATGAAATATTTGGAACTTA
  TGATTGTTAATGATCACAAAACGTATAAGAAGCATCGCTCTTCTCATGCACATACCAACAACTTTGCAAA
  GTCCGTGGTCAACCTTGTGGATTCTATTTACAAGGAGCAGCTCAACACCAGGGTTGTCCTGGTGGCTGTA
  GAGACCTGGACTGAGAAGGATCAGATTGACATCACCACCAACCCTGTGCAGATGCTCCATGAGTTCTCAA
  AATACCGGCAGCGCATTAAGCAGCATGCTGATGCTGTGCACCTCATCTCGCGGGTGACATTTCACTATAA
  GAGAAGCAGTCTGAGTTACTTTGGAGGTGTCTGTTCTCGCACAAGAGGAGTTGGTGTGAATGAGTATGGT
  CTTCCAATGGCAGTGGCACAAGTATTATCGCAGAGCCTGGCTCAAAACCTTGGAATCCAATGGGAACCTT
  CTAGCAGAAAGCCAAAATGTGACTGCACAGAATCCTGGGGTGGCTGCATCATGGAGGAAACAGGGGTGTC
  CCATTCTCGAAAATTTTCAAAGTGCAGCATTTTGGAGTATAGAGACTTTTTACAGAGAGGAGGTGGAGCC
  TGCCTTTTCAACAGGCCAACAAAGCTATTTGAGCCCACGGAATGTGGAAATGGATACGTGGAAGCTGGGG
  AGGAGTGTGATTGTGGTTTTCATGTGGAATGCTATGGATTATGCTGTAAGAAATGTTCCCTCTCCAACGG
  GGCTCACTGCAGCGACGGGCCCTGCTGTAACAATACCTCATGTCTTTTTCAGCCACGAGGGTATGAATGC
  CGGGATGCTGTGAACGAGTGTGATATTACTGAATATTGTACTGGAGACTCTGGTCAGTGCCCACCAAATC
  TTCATAAGCAAGACGGATATGCATGCAATCAAAATCAGGGCCGCTGCTACAATGGCGAGTGCAAGACCAG
  AGACAACCAGTGTCAGTACATCTGGGGAACAAAGGCTGCAGGGTCTGACAAGTTCTGCTATGAAAAGCTG
  AATACAGAAGGCACTGAGAAGGGAAACTGCGGGAAGGATGGAGACCGGTGGATTCAGTGCAGCAAACATG
  ATGTGTTCTGTGGATTCTTACTCTGTACCAATCTTACTCGAGCTCCACGTATTGGTCAACTTCAGGGTGA
  GATCATTCCAACTTCCTTCTACCATCAAGGCCGGGTGATTGACTGCAGTGGTGCCCATGTAGTTTTAGAT
  GATGATACGGATGTGGGCTATGTAGAAGATGGAACGCCATGTGGCCCGTCTATGATGTGTTTAGATCGGA
  AGTGCCTACAAATTCAAGCCCTAAATATGAGCAGCTGTCCACTCGATTCCAAGGGTAAAGTCTGTTCGGG
  CCATGGGGTGTGTAGTAATGAAGCCACCTGCATTTGTGATTTCACCTGGGCAGGGACAGATTGCAGTATC
  CGGGATCCAGTTAGGAACCTTCACCCCCCCAAGGATGAAGGACCCAAGGGTCCTAGTGCCACCAATCTCA
  TAATAGGCTCCATCGCTGGTGCCATCCTGGTAGCAGCTATTGTCCTTGGGGGCACAGGCTGGGGATTTAA
  AAATGTCAAGAAGAGAAGGTTCGATCCTACTCAGCAAGGCCCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222765 representing NM_003812
  Red=Cloning site Green=Tags(s)
  
  MKPPGSSSRQPPLAGCSLAGASCGPQRGPAGSVPASAPARTPPCRLLLVLLLLPPLAASSRPRAWGAAAP
  SAPHWNETAEKNLGVLADEDNTLQQNSSSNISYSNAMQKEITLPSRLIYYINQDSESPYHVLDTKARHQQ
  KHNKAVHLAQASFQIEAFGSKFILDLILNNGLLSSDYVEIHYENGKPQYSKGGEHCYYHGSIRGVKDSKV
  ALSTCNGLHGMFEDDTFVYMIEPLELVHDEKSTGRPHIIQKTLAGQYSKQMKNLTMERGDQWPFLSELQW
  LKRRKRAVNPSRGIFEEMKYLELMIVNDHKTYKKHRSSHAHTNNFAKSVVNLVDSIYKEQLNTRVVLVAV
  ETWTEKDQIDITTNPVQMLHEFSKYRQRIKQHADAVHLISRVTFHYKRSSLSYFGGVCSRTRGVGVNEYG
  LPMAVAQVLSQSLAQNLGIQWEPSSRKPKCDCTESWGGCIMEETGVSHSRKFSKCSILEYRDFLQRGGGA
  CLFNRPTKLFEPTECGNGYVEAGEECDCGFHVECYGLCCKKCSLSNGAHCSDGPCCNNTSCLFQPRGYEC
  RDAVNECDITEYCTGDSGQCPPNLHKQDGYACNQNQGRCYNGECKTRDNQCQYIWGTKAAGSDKFCYEKL
  NTEGTEKGNCGKDGDRWIQCSKHDVFCGFLLCTNLTRAPRIGQLQGEIIPTSFYHQGRVIDCSGAHVVLD
  DDTDVGYVEDGTPCGPSMMCLDRKCLQIQALNMSSCPLDSKGKVCSGHGVCSNEATCICDFTWAGTDCSI
  RDPVRNLHPPKDEGPKGPSATNLIIGSIAGAILVAAIVLGGTGWGFKNVKKRRFDPTQQGPI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003812
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003812.4
• RefSeq Size: 3059 bp
• RefSeq ORF: 2499 bp
• Locus ID: 8745
• UniProt ID: O75077
• Cytogenetics: 2q33.3
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. It is reported that inactivation of this gene is associated with tumorigenesis in human cancers. [provided by RefSeq, May 2013]